有限稀释定量PCR检测血清HBV-DNA的临床应用及意义

范公忍; 汪毅; 胡大荣; 邬光惠; 陈乃玲

有限稀释定量PCR检测血清HBV-DNA的临床应用及意义

计量
- 文章访问数: 2199
- HTML全文浏览量: 11
- PDF下载量: 26
- 被引次数: 0
出版历程
- 出版日期: 2000-04-20

Clinical application of limited dilution PCR to quantitative determination of serum HBV-DNA in patients with chronic hepatitis B

摘要

摘要: 探讨有限稀释定量PCR用于临床检测血清HBV -DNA ,及其对干扰素抗病毒治疗的指导意义。利用有限稀释定量PCR技术分别检测 38例HBsAg、HBeAg双阳性患者α - 2b干扰素治疗前后血清HBV -DNA含量 ,观察检测结果对治疗的指导意义。 38例患者干扰素治疗 12周HBV -DNA阴转 7例（ 18 4% ） ;治疗 2 4周阴转 11例（ 2 8 9% ）。其中病毒滴度 >2 5 0pg/ml者阴转 11 1% （ 1/9） ;病毒滴度 2 5fg/2 5 0pg/ml者阴转 2 5 0 % （ 4/16 ） ;病毒滴度<2 5fgml者阴转 46 2 % （ 6 /13） ,三组比较P <0 0 1。有限稀释定量PCR检测HBV -DNA对慢性乙型肝炎患者干扰素治疗具有一定的指导意义 ,以病毒滴度≤ 2 5 0pg/ml,干扰素治疗 2 4周疗效较佳。
- 慢性乙型肝炎 /
- 定量PCR /
- HBV-DNA /
- 干扰素
Abstract: To observe the clinical application of limited dilution PCR to quantitative determination of serum HBV-DNA, and its directive effect on interferon antivirus in patients with chronic hepatitis B HBV-DNA concentrations were quantitatively determined before and after interferon treatment in 38 patients with HBsAg (+) and HBeAg (+) by the limited dilution PCR Among 38 patients with interferon treatment 12 and 24 weeds, HBV-DNA was negative in 7 cases (18 4%) and 11 cases (28 9%) respectively There was only one case (11 5%) negative in the group of virus titer>250pg/ml (9cases) , but 4 and 6 cases (25 0% and 46 2%) negative in the groups of virus titer 25fg～250pg/ml and <25fg/ml (16 and 13 cases) (P<0 01) HBV-DNA concentration could be determined quantitatively by limited dilution PCR, and which showed some directive effect on interferon anti-virus in patients with chronic hepatitis B The patients whose virus titer≤250pg/ml and treatment 24 weeks with interferon would receive good effect
- chronic hepatitis B /
- quantitative PCR /
- HBV-DNA /
- interferon

HTML全文

参考文献(7)

[]〔1〕CrossNC QuantitativePCRtechniquesandapplication〔J〕 BrJHaema tol, 1995 , 89 (4 ) ∶5 93

[]〔2〕MacterJS , GerberMA ThepolymerasechainreactionforhepatitisBvirusDNA〔J〕 Hepatology, 1991, 13 (1) ∶189

[]〔3〕张智 , 骆抗先 , 梁炽森 , 等乙肝病毒复制是慢性肝炎活的直接原因〔J〕第一军医大学学报 , 1994, 14 (1) ∶2 0

[]〔4〕MarcellinP , PouteallM , LoriotAM , etal Adeninearabinosed 5’MonophosphateinpatientswithchronichepatitisB :comparisonoftheeffi ciencyinpatientswithhighandlowviralreplication〔J〕 Gut, 1995 , 36 (3) ∶42 2

〔5〕PerriloRP , SchiffER , GarisGL , etal Arandomizedcontrolledtrialofin terferonalfa -2baloneandafterprednisonewithdrawalforthetreatmentofchronichepatitisB〔J〕 NEng1JMed , 1990 , 32 3 (3) ∶2 95

[]〔6〕BroodMG , KaraylannisP , ThomasHC Whichpatientswithchronichep atitisBvirusinfectionwillrespondtoalfa -interferontherapy ?〔J〕 Hepatology , 1989, 10 (4 ) ∶76 1

[]〔7〕PerriloRP , RegensteninFG , PetersMG , etal Prednisonewithdrawalfol lowedbyrecombinantalpha -interferoninthetreatmentofchronictypeBhepatitis〔J〕 AnnInternMed , 1988, 10 9 (1) ∶95

施引文献

资源附件(0)

访问统计