Study on Relationship between Detection of Hepatitis B Virus Replicative Intermediates RNA and Hepatitis B Virus DNA in Peripheral Blood Mononuclear Cells in Patients with Hepatitis B Virus Infection
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摘要: 采用逆转录 -多聚酶链反应 (RT -PCR)技术检测乙肝患者外周血单个核细胞 (PBMCs)乙肝病毒复制中间体RNA(HBVRNA) ,6 0例乙肝患者 5例PBMCs中HBVRNA阳性 ,2 6例PBMCs中HBVDNA阳性 ,2 7例血清HBVDNA阳性 ;PBMCsHBVDNA阳性患者中有 4例PBMCsHBVRNA阳性 ,血清HBVDNA阳性患者中有3例PBMCsHBVRNA阳性 ;17例血清HBeAg阳性患者中有 4例PBMCsHBVRNA阳性。血清HBeAg、HBVDNA及PBMCs中HBVDNA阴性患者 ,其PBMCsHBVRNA仍可能阳性 ,其PBMCs可成为乙肝患者肝病复发和传播的来源
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关键词:
- 外周血单个核细胞 /
- 乙肝病毒 /
- 乙肝病毒复制中间体RNA /
- 多聚酶链反应
Abstract: We detected HBV replicative intermediates RNA in PBMCs in patients with HBV infection by RT PCR technology, meanwhile detected HBV DNA in PBMCs and serum by PCR technology.Five cases were detected for HBV RNA in PBMCs in 60cases of patients with HBV infection.HBV DNA were positive in both PBMCs in 26 cases and serum in 27 cases.In 26 cases whose HBV DNA in PBMCs were positive , 4 cases of HBV RNA in PBMCs were positive, In 27 cases whose HBV DNA in serum were positive, 3 cases of HBV RNA in PBMCs were positive.HBeAg was also detected in serum, in 17 cases whose HBeAg were positive, 4 cases of HBV RNA in PBMCs were positive.Though HBeAg and HBV DNA in serum, HBV DNA in PBMCs were negative in patients with HBV infection, HBV RNA in PBMCs may be positive, HBV in PBMCs could become the resource of recidimation and transmission. -
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