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The change of serum IL-12 and IL-18 level in patients with viral hepatitis and hepatic cirrhosis
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摘要: 为探讨病毒性肝炎和肝炎后肝硬化患者血清中IL - 12、IL - 18的变化规律及其临床意义 ,应用ELISA法测定血清中IL - 12及IL - 18含量。各组肝炎及肝硬化患者IL - 12及IL - 18水平明显高于对照组 （P <0 0 0 1） ,以重型肝炎组水平最高。重型肝炎死亡病例IL - 12及IL - 18明显高于生存组 （P <0 0 1）。IL - 12和IL- 18与AST、ALT及TBil正相关 ,且IL - 12与CⅣ及HA水平正相关。IL - 12、IL - 18可能参与了病毒性肝炎和肝炎后肝硬化患者的肝脏炎性损伤过程 ,重型肝炎患者联合检测血清IL - 12及IL - 18有助于判断其预后。Abstract: To investigate the changes and clinical significances of serum IL-12 and IL-18 levels in patients with viral hepatitis and hepatic cirrhosis. Serum IL-12 and IL-18 levels were determined by ELISA method in hepatitis patients, cirrhotic patients and normal controls. Concentration of serum IL-12 and IL-18 in patients with different type of viral hepatitis and cirrhosis were significantly higher than those in healthy volunteers ( P <0 01) . The levels of IL-12 and IL-18 in patients with severe hepatitis were the highest among those groups. Moreover, concentration of IL-12 and IL-18 in the dead patients with fulminant hepatitis were also significantly higher than those in the survivals. There was a close positive correlations between serum interleukins and concentration of serum AST, ALT and TBil; IL-12 also high correlate with CⅣ and HA. Our results suggested that IL-12 and IL-18 maybe participate in liver inflammatory injury process, their levels can reflect the degree of liver damage in patients with viral hepatitis and cirrhosis, and detecting the two interleukins could predict the prognosis in patient with severe hepatitis.
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Key words:
- viral hepatitis /
- interleukin-12 /
- interleukin-18
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[1]TsujiH , MukaidaN , HaradaA , etal.Alleviationoflipopoly-sac charide-inducedacuteliverinjuryinPropionibacteriumacnes-primedIFN -gamma-deficientmicebyaconcomitantreductionofTNF -alpha, IL -2, andIL -18production[J].JImmunol, 1999, 162 (2) ∶1049-55. [2]TsutsuiH , MatsuiK , KawadaN , etal.IL -18accountsforbothTNF -alpha-andFasligand-neduatedgeoatitixucoatgwatsinen dotoxin-inducedliverinjuryinmice[J].JImmunol, 1997, 159 (8) ∶3961-7 [3]TsutsuiH , KayagakiN , KuidaK , etal.Caspase-1-indepen dent, FasFasligand-mediatedIL -18secretionfrommacrophagescausesacuteliverinjuryinmice[J].Immunity, 1999, 11 (3) ∶359-67. [4]RestifoNP .Buildingbettervaccines:howapoptoticcelldeathcaninduceinflammationandactivateinnateandadaptiveimmunity[J].CurrOpinImmunol, 2000, 12 (5) ∶597-603. [5]TsutsuiH , MatsuiK , OkamuraH , etal, Pathophysiologicalrolesofinterleukin-18ininflammatoryliverdiseases[J].ImmunolRev, 2000, 174∶192-209. [6] ShibataM , HirotaM , NozawaF , etal.IncreasedconcentrationsofplasmaIL -18inatientswithhepaticdysfunctionafterhepatectomy[J].Cytokine, 2000, 12 (10) ∶1256-30. [7]AraiN , AkamatsuS , AraiS , etal.Interleukin-18incombinationwithIL -2enhancesnaturalkillercellactivitywithoutinducinglargeamountsofIFN -gammainvivo[J].JInterferonCytokineRes, 2000, 20 (2) ∶217-24. [8]NeteaMG , FantuzziG , KullbergBJ, etal.NeutralizationofIL -18reducesneutrophiltissueaccumulationandprotectsmiceagainstlethalEscherichiacoliandSalmonellatyphimuriumendotoxemia[J].JImmunol, 2000, 164 (5) ∶2644-9. -
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