乳酸菌对酒精引起的胃粘膜和肝脏损伤的保护作用

段钟平; 刘青; 金学源; 韩大康; 赵秀英; 汪俊韬

乳酸菌对酒精引起的胃粘膜和肝脏损伤的保护作用

详细信息

中图分类号: R965
计量
- 文章访问数: 2669
- HTML全文浏览量: 21
- PDF下载量: 1274
- 被引次数: 0
出版历程
- 出版日期: 2002-05-20

The protective effects of lactic acid bacterial mixture on alcohol-induced stomach mucosa and liver injury

摘要

摘要: 探讨乳酸菌混合液对酒精引起的胃粘膜及肝脏的损伤保护作用。 2 5只Wistar大鼠 ,分为乳酸菌保护组、酒精攻击组和对照组 ,共服用 5天。生化检测指标为乳酸菌乙醇脱氢酶 (ADH)、血中乙醇含量 (30分钟和 3小时 )、内毒素水平 ,并行胃、肝脏病理检查。结果显示每毫克乳酸菌蛋白质中ADH活性单位为 3 85U。服用酒精后30分钟及 3小时乳酸菌保护组动物血中乙醇浓度低于酒精攻击组 (P <0 0 0 5 ) ;乳酸菌保护组血清内毒素水平明显低于酒精攻击组 (P <0 0 1)。病理检查结果 :酒精攻击组大多数显示胃粘膜糜烂 ,上皮细胞脱落 ,肝脏严重大泡性脂变 ,而乳酸菌保护组胃和肝脏组织学基本正常。乳酸菌液通过保护胃粘膜减少酒精从胃内的吸收 ,减少细菌 /内毒素移位 ,起到预防酒精性肝损伤的作用。
- 乳酸菌 /
- 细菌内毒素移位 /
- 酒精性肝损伤
Abstract: The purpose of the present study was to determine if the lactic acid bacterial mixture could prevent ethanol induced liver injury in vivo. Twenty-five Wistar rats were divided into three groups: lactic acid bacteria feeding group; alcohol feeding group and control group. Both of lactic acid bacteria and alcohol feeding groups were exposed with alcohol (10g·kg -1 ·day -1 ) continuously for up to five days. The lactic acid bacteria feeding group firstly was treated with lactic acid bacterial mixture 1 5ml/100g before exposing alcohol for 30 min. The control group was treated with normal saline only. Following indications: alcohol dehydrogenase of the bacteria (ADH) , blood level of ethanol (30min and 3 hr) , blood level of endotoxin, serum aminotransferase and pathological evaluation of liver and stomach were observed. The ADH active units was 3 85 per milligram protein. The blood ethanol after being exposed to alcohol for 30 min and 3h was much lower than alcohol feeding group ( P <0 005) . The blood level of endotoxin was 10 5±0 3 pg/ml in lactic acid bacteria feeding group and 190±140 7 pg/ml in alcohol feeding group ( P <0 01) . The pathologic results showed most rats in lactic acid bacteria mixture feeding group had milder gastric mucosal membrane erosion and liver injury than the rats in alcohol feeding group. The lactic acid bacteria mixture may decrease alcohol absorption from stomach and reduce the translocation of bacteria and endotoxin in gut to protect alcohol-induced liver injury.
- lactic acid bacteria /
- endotoxin translocation /
- alcoholic liver injury

HTML全文

参考文献(8)

[1]Willis A. Alcohol dehydrogenate from acetic acid bacteria, membrane-bound, methods in emyology[J]. Academic Press INC, 1993, 89 (D) :450.

[2]Lowry OH, Posebrough NJ. Protein measurement with the Folin phenol reagent[J]. J Biol Chem, 1951, 193∶265-275.

[3]Goldin BR, Gorbach SL, Saxelin M, et al. Survival of lactobacillus species (strain GG) in human gastrointestinal tract[J]. Dig Dis Sci, 1992, 37∶121-128.

[4]Robins-Bronne RM, Levine MM. The fate of ingested lactobacilli in the proximal small intestine[J]. Am J Clin Nutri, 1981, 34∶514-519.

[5]Huang MT, Huang CC, Chen MY. In vivo up-take of ethanol and release of acetate in rat liver and GI [J]. Life Sci, 1993, 53 (10) ∶165-170.

[6]Hber PS, Thomas R. Metabolism of alcohol by human gastric cells: Relation to first-pass metabolism[J]. Gastraenterology, 1996, 11 (4) ∶863-870.

[7] Whitehe R. Gastrointestinal and oesophageal pathology[M]. Chur Chill Livingstone, Second edition, 1995, p249-260.

[8]Nanji AA, Urmila Khettry, Hossein SM, et al. Lactobacillus feeding reduce endotoxemia and severity of experimental alcoholic liver disease[J]. proceedings of society of experimental biology and medicine, 1994, 205 (3) ∶243-247.

施引文献

资源附件(0)

访问统计