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Research on relationship of HBV YMDD mutation and lamivudine resistance.
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摘要: 探讨拉米夫定耐药与HBVYMDD变异之间关系。对拉米夫定治疗的慢性乙型肝炎进行肝功能、乙肝病毒血清学指标、YMDD耐药基因变异检测。 10 2例慢性乙型肝炎血清中 ,HBVDNA阴性 6 3例 （6 1 8% ） ,39例阳性 ,其中HBVYMDD变异 2 2例 ,变异率为 5 6 4 % ,并与用药时间长短有关 ,9- 17个月和 18- 30个月变异检出率分别为5 1 7%和 70 % ,HBVYMDD变异组ALT为 （5 9 94± 8 6 0 ）U/L ,显著高于无HBVYMDD变异组的 （5 0 6 0± 6 12 ）U/L。基因芯片技术检测HBVYMDD变异有一定的临床意义 ,YMDD变异是HBV对拉米夫定耐药的原因之一。Abstract: To detect YMDD mutations of HBV by gene chips in patient with chronic hepatitis B treated with lamivudine and to evaluate its clinic importance. Sera of 102 patients with chronic hepatitis B treated with lamivudine 9-30 months were detected for ALT、HBV makers、YMDD mutation. In 102 patients, HBV DNA turned negative in 63 cases (61.8%) , 22 cases of YMDD mutations among 39 patients (56.4%) , its rate increasing with length of lamivudine treatment as rate was 51.7% between 9-17 months and rate 70% between 18-30 months. ALT level in patients with YMDD mutation was significant higher than the groups without YMDD mutation (59.94±8.60) U/L vs (50.60±6.12) U/L, P<0.05. Gene chips can be applied to identifying HBV YMDD mutant clinically; YMDD mutation is one of the reason that HBV resistant to lamivudine and may lead to elevation of ALT.
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Key words:
- hepatitis B virus /
- mutation /
- Lamivudine /
- gene chips
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[1] YuenMF , SablonE , HuiCK , etal.FactorsassociatedwithhepatitisBvirusDNAbreakthroughinpatientsreceivingprolongedlamivudinethera py[J].Hepatology, 2001, 34 (4) ∶785-91. [2]李宜, 高人寿, 周陶友, 等.拉米夫定治疗慢性乙型肝炎的病原学研究[J].安徽医科大学学报, 2002, 37 (3) ∶206-208. [3]杨光, 王越建, 谭家驹.一种能够平行研究大量基因的新技术———基因芯片技术的原理和应用[J].胃肠病学和肝病学杂志, 2001, 10 (2) ∶176-181. [4] BozdayiAM , UzunalimogluO , TurkyilmazAR , etal.YSDD :anovelmutationinHBVDNApolymeraseconfersclinicalresistancetolamivu dine[J].JViralHepat, 2003, 10 (4) ∶256-265. -
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