肝细胞癌组织中PTEN蛋白表达

徐华; 熊建萍

肝细胞癌组织中PTEN蛋白表达

徐华,
熊建萍

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中图分类号: R735.7
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出版历程
- 出版日期: 2004-03-20

The expression of tumor suppressor gene PTEN in hepatocellular carcinoma

摘要

摘要: 探讨抑癌基因PTEN在肝细胞癌 (HCC)组织及癌旁组织的表达、临床意义。采用免疫组织化学SP法检测PTEN。 4例正常肝组织均呈PTEN蛋白阳性 ;HCC及其癌旁肝组织中的阳性率分别为 5 8 8%(2 0 / 34)和 10 0 %(34/ 34) ,两者比较差异有显著性 (P <0 0 5 )。中分化癌阳性率为 77 8%(14 / 18) ,低分化阳性率为 2 5 %(3/ 12 ) ,两者比较差异有显著性 (P <0 0 0 1)。PTEN蛋白表达与年龄、性别、肿瘤大小、有无包膜及门脉癌栓均无明显关系 (P >0 0 5 ) ,但与HCC分化程度明显相关 ,HCC分化愈差 ,PTEN蛋白表达愈弱。PTEN蛋白表达与HCC分化程度明显相关。
- 肝肿瘤 /
- PTEN基因 /
- 蛋白表达 /
- 免疫组化
Abstract: To investigate the expression of PTEN protein in hepatocellular carcinoma (HCC) and their surrounding liver tissues as well as to elucidate its clinical significance. 34 HCC cases with their surrounding liver tissues and 4 normal liver tissues which were collected from the second affiliated hospital of jiangxi medical college were stained with PTEN mouse monoclonal antibody by immunohistochemisty (SP method) . The PTEN positive staining was found in the cytoplasm, 4 normal liver tissues and adjoining tissues of 34 carcinoma were all positive for PTEN protein. In 34 HCC, 58.8% (20/34) were positive for PTEN protein. There was a significant difference between the pattern of expression of PTEN protein in moderate differentiated carcinoma (77.8% PTEN protein) and poorly differentiated carcinoma (25% PTEN protein) . The expression of PTEN protein in HCC was not related to patient's age, sex, size, integral capsule and tumor thrombi, but was significantly associated with differentiation degrees of cancer cells (P<0.05) . The poorer was differentiation of HCC, the weaker would be the experssion of PTEN protein. The expression of PTEN protein in HCC was significantly associated with differentiation degrees of cancer cells.
- liver neoplasms /
- PTEN gene /
- protein expression /
- immunohistochemisty

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参考文献(3)

[1] Li J, Yen C, Liow D, et al. PTEN, a putative protein tyrosine phosphatase gene mutated in human brain, breast, and prostate cancer[J]. Science, 1997, 275 (5308) ∶1943-1947.

[2] Tetsuro S, Lin H, Chen XS. Differential expression of MMAC/PTEN in glioblastom a multiforme: Relationship to lacalization and prognosis[J].Cancer Res, 1999, 59 (15) ∶1820-1824.

[3] Frank BF, Huan SH J, Webster K. The bhosbhoinositol bhosphatase activity of PTEN mediates a serum sensitive G1 growth arrest in glioma cells[J].Cancer Res, 1998, 58 (18) ∶5002-5006.

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