复方中药861与961抗肝纤维化疗效比较的实验研究

赵新颜; 王宝恩; 王泰龄; 贾继东; 马雪梅

复方中药861与961抗肝纤维化疗效比较的实验研究

详细信息

中图分类号: R285
计量
- 文章访问数: 2324
- HTML全文浏览量: 10
- PDF下载量: 928
- 被引次数: 0
出版历程
- 出版日期: 2005-01-20

Comparative study of the effect of two different herbal compounds on CCl4 induced liver fibrosis in rats.

摘要

摘要: 比较复方 86 1与复方 96 1对四氯化碳实验性肝纤维化的防治效果。Wistar大鼠随机分为 4组 :对照组（10只）、四氯化碳模型组（2 0只）、复方 86 1组（15只）、复方 96 1组（15只）。实验 8周 ,取肝组织 ,应用半定量逆转录多聚酶链反应方法（RT -PCR）检测肝组织Ⅰ型前胶原、Ⅲ型前胶原、TGF - β1、TIMP - 1的mRNA水平。病理组织染色 ,记分。复方 86 1、复方 96 1显著降低Ⅰ、Ⅲ型前胶原、TGFβ1和TIMP - 1mRNA水平（P <0 0 1）。肝组织病理纤维化分级表明 :两复方抑制纤维结缔组织增生程度相似。复方 96 1与复方 86 1抗纤维化效果相近
- 四氯化碳 /
- 肝纤维化 /
- 复方中药 /
- 转化生长因子β1 /
- 金属蛋白酶组织抑制因子-1
Abstract: To compare the anti-fibrotic effects of two Recipes of commonly used Chinese traditional herbal compounds.Sixty male Wistar rats were divided into four groups:control group (10 rats) , model group (20 rats) , group treated with compound 861 (15 rats) and group treated with compound 961 (15 rats) .Hepatic fibrosis was induced by carbon tetrachloride (CCl4) subcutaneous injection for 8 weeks.Herbal compounds 861 and 961 were administered by gastric gavage respectively.At the end of 8 weeks, the gene mRNA level of procollagen Ⅰ, procollagen Ⅲ and transforming growth factor beta one (TGF-β1) and tissue inhibitor of metalloproteinase one (TIMP-1) was examined by means of reverse transcription polymerase chain reaction (RT-PCR) .Histopathology of each group was assessed with HE, Masson and Sirus red staining.Compound 861 and 961 could decrease all mRNA level: procollagen Ⅰ, procollagen Ⅲ mRNA level, TGF-β1 and TIMP-mRNA level (P<0.01) .Compound 861 and Compound 961 could significantly suppress the fibrotic proliferation by pathological assays.It concluded that Compound 861 has the similar antifibotic effect with Compocmd 961.
- carbon tetrachloride /
- liver fibrosis /
- herbal compounds /
- transforming growth factor beta one (TGF-β1) /
- tissue inhibitor of metalloproteinase one (TIMP-1)

HTML全文

参考文献(14)

[1] BrandstenC , LundmarkC , ChristerssonC , etal.Expressionofcollagenalphal (I) mRNAvariantsduringtoothandboneformationintherat.JDentRes.1999Jan;78 (1) ∶11-9.

[2] GlumoffV , MakelaJK , VuorioE .CloningofcDNAfouratproalphal (Ⅲ) collagenmRNA .DifferentexpressionpatternsoftypeIandtypeⅢcollagenandfibronectin genesinexperimentalgranulationtissue[J].BiochimBiophysActa, 1994, 1217 (1) ∶41-8.

[3] QianSW , KondaiahP , RobertsAB , etal.cDNAcloningbyPCRofrattransforminggrowthfactorbeta-1[J].NucleicAcidsRes, 1990, 18 (10) ∶3059.

[4] OkadaA , GarnierJM , VicaireS , etal.CloningofthecDNAencodingrattissueinhibitorofmetalloproteinasel (TIMP -1) , aminoacidcom parisonwithotherTIMPs, andgeneexpressioninrattissues[J].Gene, 1994, 147 (2) ∶301-2.

[5] TsoJY , SunXH , KaoTH , etal.Isolationandcharacterizationofratandhumanglyceraldehyde-3- phosphatedehydrogenasecDNAs:ge nomiccomplexityandmolecularevolutionofthegene[J].NucleicAcidsRes.1985, 13 (7) ∶2485-502.

[6] IimuroY , NishioT , MorimotoT , etal.Deliveryofmatrixmetallopro teinase-1attenuatesestablishedliverfibrosisintherat[J].Gastroen terology, 2003, 124 (2) ∶445-58.

[7] LeeHS , HuangGT , MiauLH , etal.Expressionofmatrixmetallopro teinasesinspontaneousregressionofliverfibrosis[J].Hepatogas troenterology, 2001, 48 (40) ∶1114-7.

[8] BonisPA , FriedmanSL , KaplanMM .Isliverfibrosisreversible[J].NEnglJMed, 2001, 344 (6) ∶452-4.

[9] KondouH , MushiakeS , EtaniY , etalAblockingpeptidefortrans forminggrowthfactor-betalactivationpreventshepaticfibrosisinvi vo[J].JHepatol, 2003, 39 (5) ∶742-8.

[10] IredaleJP , BenyonRC , ArthurMJ, etal.Tissueinhibitorofmetallo proteinase-1messengerRNAexpressionisenhancedrelativetointer stitialcollagenasemessengerRNAinexperimentalliverinjuryandfi brosis[J].Hepatology, 1996, 24 (1) ∶176-84.

[11] MurphyFR , IssaR , ZhouX , etal.Inhibitionofapoptosisofactivatedhepaticstellatecellsbytissueinhibitorofmetalloproteinase-1isme diatedviaeffectsonmatrixmetalloproteinaseinhibition:implicationsforreversibilityofliverfibrosis[J].JBiolChem, 2002, 277 (13) ∶11069-76.

[12]王宝恩, 赵洪涛, 王泰龄, 等.复方861对肝炎肝纤维化疗效的病理组织学分析[J].中华肝脏病杂志, 1997, 5 (2) ∶77.

[13]贾继东, 王宝恩, 董忠, 等.中药861合剂对免疫损伤性肝纤维化大鼠肝脏Ⅰ、Ⅲ、Ⅳ型胶原及TGF -βmRNA的影响[J].中华肝脏病杂志, 1996, 4 (4) ∶214-216.

[14]尤红, 王宝恩, 王泰龄, 等.复方861对肝星状细胞的增殖和凋亡的干预作用[J].中华肝脏病杂志, 2000, 8 (2) ∶78-80.

施引文献

资源附件(0)

访问统计