复方中药861与961抗肝纤维化疗效比较的实验研究

赵新颜; 王宝恩; 王泰龄; 贾继东; 马雪梅

复方中药861与961抗肝纤维化疗效比较的实验研究

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中图分类号: R285
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出版历程
- 出版日期: 2005-01-20

Comparative study of the effect of two different herbal compounds on CCl4 induced liver fibrosis in rats.

摘要

摘要: 比较复方 86 1与复方 96 1对四氯化碳实验性肝纤维化的防治效果。Wistar大鼠随机分为 4组 :对照组（10只）、四氯化碳模型组（2 0只）、复方 86 1组（15只）、复方 96 1组（15只）。实验 8周 ,取肝组织 ,应用半定量逆转录多聚酶链反应方法（RT -PCR）检测肝组织Ⅰ型前胶原、Ⅲ型前胶原、TGF - β1、TIMP - 1的mRNA水平。病理组织染色 ,记分。复方 86 1、复方 96 1显著降低Ⅰ、Ⅲ型前胶原、TGFβ1和TIMP - 1mRNA水平（P <0 0 1）。肝组织病理纤维化分级表明 :两复方抑制纤维结缔组织增生程度相似。复方 96 1与复方 86 1抗纤维化效果相近
- 四氯化碳 /
- 肝纤维化 /
- 复方中药 /
- 转化生长因子β1 /
- 金属蛋白酶组织抑制因子-1
Abstract: To compare the anti-fibrotic effects of two Recipes of commonly used Chinese traditional herbal compounds.Sixty male Wistar rats were divided into four groups:control group (10 rats) , model group (20 rats) , group treated with compound 861 (15 rats) and group treated with compound 961 (15 rats) .Hepatic fibrosis was induced by carbon tetrachloride (CCl4) subcutaneous injection for 8 weeks.Herbal compounds 861 and 961 were administered by gastric gavage respectively.At the end of 8 weeks, the gene mRNA level of procollagen Ⅰ, procollagen Ⅲ and transforming growth factor beta one (TGF-β1) and tissue inhibitor of metalloproteinase one (TIMP-1) was examined by means of reverse transcription polymerase chain reaction (RT-PCR) .Histopathology of each group was assessed with HE, Masson and Sirus red staining.Compound 861 and 961 could decrease all mRNA level: procollagen Ⅰ, procollagen Ⅲ mRNA level, TGF-β1 and TIMP-mRNA level (P<0.01) .Compound 861 and Compound 961 could significantly suppress the fibrotic proliferation by pathological assays.It concluded that Compound 861 has the similar antifibotic effect with Compocmd 961.
- carbon tetrachloride /
- liver fibrosis /
- herbal compounds /
- transforming growth factor beta one (TGF-β1) /
- tissue inhibitor of metalloproteinase one (TIMP-1)

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参考文献(14)

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