转化生长因子β1及其Ⅰ、Ⅱ型受体在肝细胞癌中的表达

凌云峰; 冯震博; 何如昆; 吕自力

转化生长因子β1及其Ⅰ、Ⅱ型受体在肝细胞癌中的表达

基金项目:

广西壮族自治区科技厅资助 (编号 0 1 350 4 6)；

详细信息

中图分类号: R735.7
计量
- 文章访问数: 2220
- HTML全文浏览量: 27
- PDF下载量: 731
- 被引次数: 0
出版历程
- 出版日期: 2005-01-20

Expression of TGFβ1、TGFβRⅠ and TGFβRⅡ in hepatocellular carcinoma

Research funding:

摘要

摘要: 运用免疫组化方法对 36例HCC和 4 2例癌旁肝组织的TGFβ1及其Ⅰ、Ⅱ型受体表达情况进行研究。TGFβ1在 36例肝癌组织和 4 2例癌旁肝组织的阳性率分别为 6 9 4 %、81% ,两者差异无显著性 (P >0 0 5 ) ,半定量分析TGFβ1在肝癌组织表达阳性面积百分比为 (17 2 3± 11 78) % ,癌旁肝组织为 (38 4 7± 15 34) % ,差异有显著性意义 (P <0 0 5 )。TGFβRⅠ在HCC、癌旁肝组织的阳性率分别为 4 1 7%、73 8% ,TGFβRⅡ在HCC、癌旁肝组织阳性率分别为 4 7 2 %、85 7% ,差异均有非常显著性意义 (P <0 0 1)。肝癌细胞和癌旁肝细胞中均高度表达TGFβ1,两者阳性率无差异 ,但阳性面积百分比后者显著高于前者 ,提示TGFβ1表达降低可能与肝细胞的恶性转化和生长失控有关。TGFβ1过度表达而不能抑制癌细胞的恶性增殖 ,可能与肝癌细胞中TGFβRⅠ和TGFβRⅡ表达降低有关
- 转化生长因子β1 /
- 转化生长因子β受体Ⅰ /
- 转化生长因子β受体Ⅱ /
- 肝细胞癌 /
- 癌旁肝组织
Abstract: Immunohistochemical technique was used to detect the expression of TGFβ1、TGFβRⅠ and TGFβRⅡ in 36 cases HCC and 42 cases paratumorous liver tissue. There was no difference in the positive rates of TGFβ1 expressioin between HCC tissue (69.4%) and paratumorous liver tissue (81.0%) (P>0.05) . However, by the semi-quantiative analysis, the positive area percentage of TGFβ1 expression decreased in HCC tissue (17.23±11.78%) compared with that in paratumorous liver tissue (38.47±15.34%) (P<0.05) . The positive rate of TGFβRⅠ expression decreased significantly in HCC tissue (41.7%) compared with that in paratumorous liver tissue (73.8%) (P<0.01) . The positive rates of TGFβRⅡ expression decreased significantly in HCC tissue (42.7%) compared with that in paratumorous liver tissue (85.7%) (P<0.01) . Both HCC and paratumorous liver tissue can express high level of TGFβ1, but the positive area percentage of TGFβ1 expression in the former decrease significantly compared with that in the later, which implies the decrease of TGFβ1 expression may be involved in liver cells malignant transformation and uncontrolled growth. Decreasing expression of TGFβRⅠ and TGFβRⅡ in HCC may be the reasons for escaping the negative regulation of TGFβⅠ.
- transforming growth factor β1 (TGFβ1) /
- TGFβRⅠ /
- TGFβRⅡ /
- hepatocellular carcinoma /
- paratumorous liver tissue

HTML全文

参考文献(12)

[1] LutgensE , gijbelsM , SmookM , etal.Transforminggrowthfactor- (beta) mediatesbalancebetweeninflammationandfibrosisduringplaqueprogression.Arteriosclerosis, ThrombosisandVascularBiology[J].Dallas, 2002, 22 (6) ∶975-78.

[2]钱建民, 王学浩.肝癌患者转化生长因子β1及其受体的表达意义[J].中华实验外科杂志, 2000, 17 (6) ∶535-536.

[3]刘超, 区庆嘉.TGF -β1抑制肝癌细胞株BEL -7402的增殖[J].肝胆外科杂志, 1999, 7 (3) ∶219-221.

[4] RomieeuR , LacabanneV , KayibandaM , etal.Criticalstagesoftu morgrowthregulationtransgenicmiceharboringahepatocellularcar cinomarevealedbydistinctpatternsoftumornecrosisfactor-alphaandtransforminggrowthfactor-betamRNAproduction[J].InIm munol, 1997, 9∶1409-1412.

[5]刘超, 陈双, 区庆嘉.转化生长因子β1及其Ⅱ型受体在肝细胞癌中的基因表达[J].中华外科杂志, 1999, 37 (12) ∶740-742.

[6] BedossaP , PeltierE , TerrisB , etal.Transforminggrowthfactor-betal (TGF -β1) andTGF -β1receptorsinnormal, cirrhotic, andneoplastichumanlivers[J].Hepatology, 1995, 21∶760.

[7] KehrlJH , WakefieldLM , KobertsAB , etal.TransforminggrowthfacotrβisanimportantimmunomodulatoryproteinforhumanB -lymphocytes[J].JImmunol, 1986, 137∶3855.

[8] RobertsAB , SpornMB , AssoianPK , etal.Transforminggrowthfactortype-β:Rapidinductionoffibrosisandangiogenesisinvivoandstimulationcollagenformatoninvitro[J].ProNatlAcadSciUSA , 1986, 83∶4167.

[9] WangJ, HanW , ZborowskaJL , etal.Reducedexpressionoftrans forminggrowthfactorβtypeⅠreceptorcontributestothemalignancyofhumancoloncarcinomacells[J].JBiolChem, 1996, 271∶17366-17371.

[10] KimIY , AhnHJ, ZelherDJ, etal.Geneticchangintransforminggrowthfactorbeta (TGF -beta) receptortypeⅠgenecorrelateswithinsensitivitytoTGF -beta1inhumanprostatecancercells[J].CanRes, 1996, 56∶44.

[11] InagakiM , MoustakasA , LinHY , etal.Growthinhibitionbytransforminggrowthfactorbeta (TGF -beta) typeIisrestoredinTGF -beta-resistanthepatomacellsafterexpressionofTGF -betareceptortypeⅡcDNA[J].ProcNatlAcadSci, 1993, 90 (11) ∶5359-5363.

[12] KnausPI , LindemanD , DeCoteauJF , etal.AdominantinhibitorymutantofthetypeⅡtransforminggrowthfactorbetareceptorinthemalignantprogressionofacutaeousT -celllymphoma[J].MolCellBiol, 1996, 16 (7) ∶3480-3489.

施引文献

资源附件(0)

访问统计