骨髓干细胞在大鼠肝再生环境中的分化

展玉涛; 陈红松; 刘宾; 魏来; 王宇

骨髓干细胞在大鼠肝再生环境中的分化

基金项目:

国家863计划(2001AA216031)；

详细信息

中图分类号: R329
计量
- 文章访问数: 2040
- HTML全文浏览量: 15
- PDF下载量: 945
- 被引次数: 0
出版历程
- 出版日期: 2006-02-20

Differentiation of rat bone marrow stem cells in liver regeneration.

Research funding:

摘要

摘要: 研究大鼠骨髓干细胞在部分肝切除后肝再生环境中的分化。从部分肝切除模型大鼠的胫骨中提取骨髓细胞,应用流式细胞仪富集骨髓干细胞,以PKH26-GL体外标记后通过门静脉进行自体移植,2周后行白蛋白和角蛋白8免疫组化检查。结果肝板肝细胞间PKH26-GL标记骨髓干细胞表达白蛋白、角蛋白8。提示骨髓干细胞在部分肝切除后肝再生环境中能分化为肝细胞,骨髓干细胞可能参与部分肝切除后的肝再生过程。
- 骨髓干细胞 /
- 肝再生
Abstract: To investigate the differentiation of rat bone marrow stem cells in liver regeneration.Bone marrow cells were obtained from the tibia of rat with partial hepatectomy.The bone marrow stem cells were enriched from the bone marrow cells by fluorescence-activated cell sorting.The bone marrow stem cells were labeled with PKH26-GL in vitro and then autotransplanted by portal vein injection.After 2 weeks, the livers were examined by albumin and ck 8 immunohistochemistry.The results show that there were PLH26-GL-labeling cells among hepatocytes in the liver sections, and some of the cells expressed albumin and ck 8.The results suggest that rat bone marrow stem cells can differentiate into hepatocyte in regenerative environment after partial hepatectomy, bone marrow stem cells may participate in liver regeneration after partial hepatectomy.
- bone marrow stem cells /
- liver regeneration

HTML全文

参考文献(8)

[1]Theise ND, Badve S, Saxena R, et al.D erivation of hepatocytesfrom bone marrow cells in m ice after rad iation-induced myeloab la-tion[J].Hepatology, 2000, 31 (1) ∶235-240.

[2]A lisonMR, Pou lsom R, Jeffery R, et al.Hepatocytes from non-he-patic adu lt stem cells[J].Nature, 2000, 406 (6793) ∶257.

[3]Theise ND, N immakayalu M, Gardner R, et al.L iver from bonemarrow in humans[J].Hepatology, 2000, 32 (1) ∶11-16.

[4]Petersen BE, Bowen WC, Patrene KD, et al.Bone marrow as a po-tential source of hepatic oval cells[J].Science, 1999, 284 (5417) ∶1168-1170.

[5]展玉涛, 魏来, 陈红松, 等.用流式细胞仪富集大鼠骨髓干细胞[J].免疫学杂志, 2003, 19 (6) ∶471-473.

[6]Sell S.Heterogeneity and plasticity of hepatocyte lineage cells[J].Hepatology, 2001, 33 (3) ∶738-50.

[7]Hendrikx PJ, Martens CM, Hagenbeek A, et al.Hom ing of fluores-cently labeled murine hematopoietic stem cells[J].Exp Hematol, 1996, 24 (2) ∶129-40.

[8]Oh D J, Lee GM, Francis K, et al.Phototoxicity of the fluoresentm embrane dyes PKH2 and PKH26 on the human hematopoietic kG1 a progen itor cell line[J].Cytom etry, 1999, 36 (4) ∶312-18.

施引文献

资源附件(0)

访问统计