PDF下载 ( 160 KB)
The effects of cisplatin and matrine on telomerase activity in human hepatoma cell line HepG2
-
摘要: 观察顺铂、苦参素对人肝癌细胞株HepG2端粒酶活性的影响,进一步探讨两种药物的可能作用机制。采用人肝癌细胞株HepG2作为研究对象,MTT法检测不同浓度和作用时间的药物对细胞增殖的抑制作用;流式细胞仪分析细胞周期的分布及凋亡;PCR-ELISA法检测端粒酶活性的变化。两种药物均具有抗HepG2细胞增殖、阻滞细胞周期、诱导凋亡及抑制端粒酶活性的作用。对端粒酶活性的抑制可能是两种药物发挥抗肿瘤作用的机制之一。Abstract: To investigate the anti-cancer mechanism of cisplatin and matrine by observing their effects on telomerase activity.The inhibition of proliferation in HepG2 cells were estimated by MTT-test.Distribution of cell cycle and apoptosis were analysed using flow cytometry.The changes of telomerase activity were detected by PCR-ELISA method.The two drugs could inhibit the proliferation of HepG2, block cell cycle, induce apoptosis and inhibit the activity of telomerase.The inhibition of telomerase activity may be one of the mechanisms of the anti-cancer effect of the two drugs.
-
Key words:
- hepatocellular carcinoma /
- telomerase /
- cisplatin /
- matrine
-
[1]于勇, 卢佃华, 杜鹏程, 等.顺铂治疗胃癌的临床应用进展[J].中国药师, 2004, 7 (3) ∶199-201. [2]李正蓉.苦参素的药理与临床研究进展[J].华西药学杂志, 2003, 18 (6) ∶435-437. [3]汤为学, 骆云鹏, 王瑞雪.人实体瘤抗癌药物敏感试验MTT法的建立[J].重庆医科大学学报, 1992, 17 (21) ∶103-104. [4]Chou TC, Talalay P.Quantitative analysis of dose-effects relation-sh ips∶the comb ined effects of mu ltiple drugs on enzym e inh ib itors[J].Adv Enzym e Regu l, 1984, 22 (1) ∶27-29. [5]H isieh HF, Harn HJ, Ch iu SC, et al.Telom elase activity correlatesw ith cell cycle regu lators in human hepatocellu lar carcinoma[J].L iv-er, 2000, 20 (1) ∶143-145. [6]Kyo S, Takakura M, Taira T, et al.Sp1 cooperates w ith c-Myc toactivate transcription of the human telom erase reverse transcriptasegene (hTERT) [J].Nucleic Acids Res, 2000, 28 (3) ∶669-677. [7] H li L iu JP S inaling on telom erase∶a master sw itch in cell aging andimmortalization[J].B iogerontology, 2002, 3 (1) ∶107-116. -
本文二维码
计量
- 文章访问数: 1998
- HTML全文浏览量: 22
- PDF下载量: 768
- 被引次数: 0
下载:
