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Association of the genetic polymorphisms of interleukin (IL) -10 with susceptibility to HBV-induced liver cirrhosis
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摘要: 探讨白细胞介素10（IL-10）基因多态性与乙型肝炎肝硬化易感性的关系。采用聚合酶链反应-限制性片段长度多态性（PCR-PFLP）分析方法,检测100例乙肝肝硬化患者及124例健康对照组IL-10基因启动子-1082G/A、-592A/C位点的基因多态性,并确定了其基因型和等位基因频率的分布。肝硬化组与对照组IL-10基因启动子-592A/C位点基因型分布频率和等位基因频率差异无显著性（P>0.1）,肝硬化组-1082G/A位点AA基因型频率及A等位基因频率高于对照组（P<0.05）,G等位基因相对于A等位基因患肝硬化的机会比为0.373（95%C I:0.1660.838）。IL-10基因多态性与肝硬化易感性相关,基因启动子-1082G/A位点AA基因型携带者肝硬化易感性高。Abstract: To study the possible association of the genetic polymorphism of interleukin (IL) -10 with the susceptibility to HBV-induced liver cirrhosis.Peripheral blood samples were collected from 100 patients with HBV-induced liver cirrhosis and 124 healthy controls.The polymorphisms at position-1082G/A, -592A/C of the promoter of IL-10 gene were determined by polymerase chain reaction-restriction fragment length polymorphisms analysis (PCR-RFLP) .For position-592A/C, the genotype frequency and allele frequencies were not significantly different between the cirrhosis group and healthy group (P>0.1) .For position-1081G/A, relative higher AA genotype frequency and an A allele frequency were found in cirrhosis group (P<0.05) .And the rate to get liver cirrhosis was 0.373 among people with G allele compared with A allele (95%confidence interval was 0.116-0.838) .The results suggested that the genotype of IL-10-1082G/A positiion was associated with the susceptibility to HBV-induced liver cirrhosis.
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Key words:
- liver cirrhosis /
- interleukin-10 /
- gene polymorphisms /
- hepatitis B virus
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