原发性肝癌患者外周血中AFPmRNA检测的临床意义

张淑文; 张建东; 石欣荣; 卢晋英

原发性肝癌患者外周血中AFPmRNA检测的临床意义

详细信息

中图分类号: R735.7
计量
- 文章访问数: 2191
- HTML全文浏览量: 11
- PDF下载量: 978
- 被引次数: 0
出版历程
- 出版日期: 2007-04-20

Detection of alpha-fetoprotein messenger RNA in peripheral blood of patients with primary hepatic cancer

摘要

摘要: 通过定量检测AFPmRNA在原发性肝癌（PHC）患者外周血中的表达,探讨AFPmRNA作为PHC患者早期诊断和微转移指标的可能性。建立荧光定量聚合酶链式反应（FQ-PCR）,检测58例外周血AFPmRNA的表达量。其中,30例PHC患者中19例AFP≥20ng/m l,23例外周血中AFPmRNA有表达;肝癌患者外周血中AFPmRNA的基因拷贝数与肝癌患者有肝内外转移有明显相关,而肝炎及健康对照组外周血中AFPmRNA均未表达,肝硬化组有一例表达。应用荧光定量PCR检测PHC外周血中AFPmRNA表达情况,可以作为早期反映PHC患者外周血中是否存在肝癌细胞和发生微转移的早期灵敏指标。
- 原发性肝癌 /
- 荧光定量聚合酶链式反应 /
- 甲胎蛋白mRNA /
- 外周血
Abstract: To investigate the expression of α-fetoprotein (AFP) mRNA in peripheral blood of primary hepatic cancer (PHC) and probe into the possibility of AFP as a sign of diagnosing PHC and a marker of micrometasis in PHC.A sensitive FQ-PCR system was set up to detect the AFPmRNA in peripheral blood of 58 specimen, As the shown in the datas, detection of AFPmRNA expression was positive in 23 of 30 PHC patients, and AFP20ng/ml were found in 19 patients.The presence of AFPmRNA correlated with the extrahepatic or intrahepatic metasasis, whereas the expression of AFPmRNA in peripheral blood may be used as an indicater of citculating PHC cells, which might predicate hematogenous spreading metasis of tumor cells in PHC patients.
- primary hepatic cancer /
- polymerase chain reaction /
- alpha-fetoproteins mRNA /
- peripheral blood

HTML全文

参考文献(7)

[1]Cherqui D, Piedbois P, Pierga JY, et al.Multimodal adjuvant treat-ment and liver transplantation for advanced hepatocellular carcinoma[J].Cancer, 1994, 73 (11) ∶2721-2726.

[2]仉红刚, 史国刚, 王景明, 等.逆转录多聚酶链反应检测白血病多药耐药性基因表达[J].中华内科杂志, 1995, 34 (7) ∶463-466.

[3]Matsumura M, Nlwa Y, Kato N, et al.Detection of Alpha-fetopro-tein mRNA an Indication of Hematogenous Speading HepatocellularCarcinoma in Circulation Possible Predictor of Metastic HepatocellularCarcinoma[J].Hepatology, 1994, 20 (6) ∶1418-1420.

[4]Komeda T, Fukuda Y.Sensitive detection of circulation hepatocellu-lars in peripheral venous blood[J].Cancer, 1995, 75 (9) ∶2214-2219.

[5]杨业发.寻找肝癌转移标记[J].国外医学肿瘤分册, 1997, 24 (4) ∶231-234.

[6]Luzzi KJ, MacDonald I, Schmidt E, et al.Multistep nature of meta-static inefficiency.Dormaney of solitary cells after successful extrava-sation and limited survival of early micrometastases[J].AmJ Pathol, 1998, 153 (3) ∶865-873.

[7]薛开先.PCR技术与实体瘤转移的研究[J].国外医学遗传学分册, 1997, 20 (5) ∶227-230.

施引文献

资源附件(0)

访问统计