乙型肝炎患者血清趋化因子CXCL16的检测及其意义

杨勇; 刘佩芝; 詹爱琴

乙型肝炎患者血清趋化因子CXCL16的检测及其意义

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中图分类号: R512.6
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出版历程
- 出版日期: 2008-02-20

The assay and significance of CXCL16 in serum of hepatitis B patients.

摘要

摘要: 目的探讨乙肝患者血清趋化因子CXCL16的变化规律及其临床意义。方法用酶联免疫吸附法（ELISA）检测血清CXCL16浓度;荧光定量聚合酶链反应法（FQ-PCR）检测HBV-DNA;全自动生化仪检测肝功能。结果对照组（n=25）、急性乙肝组（n=24）、慢性乙肝组（n=32）CXCL16浓度分别为1.676±0.766（ng/ml）、2.150±0.714（ng/ml）、2.417±0.537（ng/ml）。急、慢性乙肝组CXCL16浓度显著高于对照组（P值分别为0.015、0.000）。急、慢性乙肝组之间比较,差异无统计学意义（P=0.142）。HBeAg阳性乙肝组（n=26）与HBeAg阴性乙肝组（n=30）比较,差异无统计学意义（P=0.741）。CXCL16浓度与ALT水平无相关性（r=-0.46,P=0.736）,与HBVDNA拷贝数也无相关性（r=-0.191,P=0.158）,但CXCL16有随病情加重而增高的趋势。结论CXCL16可能参与了乙肝的炎症损伤机制;ALT、HBVDNA及HBeAg与CXCL16浓度变化无明显相关。
- 趋化因子 /
- CXCL16 /
- 乙型肝炎
Abstract: Objective To investigate the change and clinical significance of serous chemokine CXCL16 in patients with hepatitis B.Methods The serous concentration of CXCL16 was detected by enzyme linked immunosorbent assay (ELISA) ;HBV-DNA was detected by fluorescent quantitive polymerase chain reaction (FQ-PCR) ;Liver function was assayed by automatic biochemistry analyzer.Results Concentration of serous CXCL16 in normal group (n=25) 、AHB (acute hepatitis B) group (n=24) and CHB (chronic hepatitis B) group (n=32) was 1.676±0.766 (ng/ml) 、2.150±0.714 (ng/ml) and 2.417±0.537 (ng/ml) respectively.The concentration of CXCL16 in AHB and CHB groups were both significantly higher than that of normal group, (P=0.015, 0.000, respectively) .However, the disparity of CXCL16 concentration between AHB and CHB group isn't statistically significant (P=0.142) .Likewise, no statistical significance of the difference of CXCL16 was found between HBeAg positive (n=26) and negative (n=30) patients group (P=0.741) .There is no correlation between the concentration of CXCL16 and the level of ALT (r=-0.46, P=0.7365) , so as the association of CXCL16 and HBVDNA (r=-0.191, P=0.158) .Nevertheless, the concentration of CXCL16 is raised as the pathogenetic condition aggravated.Conclusion It is possible that CXCL16 play a role in liver inflammatory in hepatitis B.The change of the serous concentration of CXCL16 isn't evidently related with that of ALT、HBVDNA and HBeAg.
- chemokine /
- CXCL16 /
- hepatitis B

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参考文献(8)

[1]Kunkel E J, Boisvert J, Murphy K, et al.Expression of the Chemo-kine Receptors CCR4, CCR5, and CXCR3 by Human Tissue-Infil-trating Lymphocytes[J].Am J Phathol, 2002, 160 (1) ∶347-355.

[2]Matloubian M, David A, Engel S, et al.Atransmembane CXC Che-mokine is a ligand for HIV-coreceptor BONZO[J].Nat Immunol, 2000, 1 (4) ∶298-304.

[3]Heydtmann M, Lalor P, Ekstan J, et al.CXC Chemokine Ligand 16Promotes Integrin-Mediated Adhesion of Liver-Infiltrating Lym-phocytes to Chlangioicytes and Hepatocytes within the Inflamed Hu-man Liver[J].J Immunol, 2005, 174∶1055-1062.

[4]中华医学会.病毒性肝炎防治方案[J].中华传染病杂志, 2001, 19 (1) ∶56.

[5]吴玉章.基础免疫学[M].北京:科学出版社, 2003∶871-872.

[6]Loetscher M, Gerber B.Chemokine receptor specific for IP-10 andMig:structure, function, and expression in activatedT-lymphocytes[J].J Exp Med, 1996, 184∶963-969.

[7]Peter J, Kyle J, Paul T, et al.A Disintegin and Metalloproteinase10-Mediated Cleavage and Shedding Regulates the Cell Surface Ex-pression of CXC Chemokine Ligand 16[J].J Immunol, 2004, 172∶3678-3685.

[8]彭文伟.传染病学[M].第六版, 北京:人民卫生出版社, 2003∶31.

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