角蛋白K18及其33、52位丝氨酸磷酸化在HBV感染肝脏疾病中的表达及意义

李娟; 石英; 武聚山; 绳波; 于红卫; 吴昊; 陈新月; 陈德喜

角蛋白K18及其33、52位丝氨酸磷酸化在HBV感染肝脏疾病中的表达及意义

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中图分类号: R512.62
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出版历程
- 出版日期: 2008-04-20

The expression of Keratin 18 and its Ser33、Ser52 phosphorylation level changes in HBV infected liver disease and significance

摘要

摘要: 目的探索K18磷酸化ser33和ser52水平与HBV感染肝脏疾病的关系。方法应用组织化学免疫荧光法检测HBV感染后不同进程肝组织中K18及其磷酸化ser33和ser52的表达及其相对亚细胞定位;Western blot方法检测HBV感染后不同进程肝组织中K18及其磷酸化ser33和ser52水平。结果组织化学免疫荧光结果显示K18及其磷酸化Ser33和Ser52在所有肝组织都有表达,K18的表达没有明显差异,K18磷酸化Ser33和Ser52在正常细胞中表达较弱,位于细胞膜周围,在慢性HBV肝病时弥漫分散于细胞浆中且表达增强,重型肝炎表达最高;Western blot结果显示与对照相比,K18的Ser52水平被显著诱导但在不同程度肝脏疾病的表达无显著差异,重型肝炎时,K18的Ser33水平被显著诱导。结论K18高度磷酸化可能是HBV感染后肝脏疾病病情进展的标志。
- 细胞角蛋白18(K18) /
- K18磷酸化Ser33和Ser52 /
- HBV感染后肝脏疾病
Abstract: Objective To investigate the relationship of Ser-33 and Ser-52 phosphorylated K18 and the progression of HBV infected human liver disease.Methods The expression and localization of K18 and Ser-33, 52 phosphorylated K18 of different progression of HBV infected human severe hepatitis were detected by histochemistry;The expression of K18 and Ser-33, 52phosphorylated K18 of different progression of HBV infected human liver disease were detected by Western Blot.Results The expression level of K18 in the liver cells have no association with progression of HBV hepatitis.Ser-33 and Ser-52 expressed very low in normal cells and locatedaround cytoplasmic membrane;Ser-33 and Ser-52 were induced overexpression in whole cellcytoplasm in chronicity HBV hepatitis.The expression of Ser-33 and Ser-52 reached a highest levels in severe hepatitis.The levels of Ser-33 and Ser-52 have significative differences among different states of liver diseases.Ser-52 phosphorylated K18 different states of liver diseases was distinctly induced in contrast to the healthy liver tissue, bu the levels had no difference.The levels of Ser-33 phosphorylated K18 were distinctly induced in severe hepatitis.Conclusion The phosphroylation of K18 could be a marker of progression of HBV infected human liver disease.
- K18 /
- K18 Ser-33 phosphorylated /
- K18 Ser-52 phosphorylated

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参考文献(14)

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