肝纤维化大鼠肝组织中NF-κB及ERK-1mRNA的表达

胡泰洪; 蒋祥虎; 蒋跃明; 周敏; 徐敏; 朱锐; 杨玲

肝纤维化大鼠肝组织中NF-κB及ERK-1mRNA的表达

1. 无锡市传染病医院
2. 华中科技大学同济医学院附属协和医院

基金项目:

江苏省中医药管理局资助(No:H05096)；

详细信息

中图分类号: R575.2
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出版历程
- 出版日期: 2009-01-20

Expression of NF-κB and ERK-1mRNA in rat with liver fibrosis

Research funding:

摘要

摘要: 目的探讨核转录因子-κB（NF-κB）及细胞外信号调节激酶（ERK）mRNA在CCl4致实验性肝纤维化大鼠肝组织中的表达及其与肝纤维化的关系。方法建立CCl4致大鼠实验性肝纤维化的模型,用免疫组化法比较正常组,模型组的α平滑肌肌动蛋白（α-SMA）的表达,用RT-PCR法比较各组肝组织NF-κB以及ERK-1mRNA的表达。结果模型组中,ERK-1及NF-κBmRNA的表达均增高。结论ERK通路介导的促增殖作用与NF-κB通路介导的促炎症反应,促进HSC的激活、增殖,在肝纤维化的发生发展中均是不可或缺的。
- 核转录因子κB /
- 细胞外信号调节激酶-1 /
- 肝纤维化
Abstract: Objective To evaluate Expression of NF-κB and ERK-1mRNA in rat with liver fibrosis.Methods Liver fibrosis in rats were induced by carbon tetrachloride (CCl4) .Rats were divided into two groups: normal control group, CCl4 model group.The expression of alpha-Smooth Muscle Action were detected by immunohistochemistry.The mRNA expression of nuclear factor kappaB (NF-κB) and extracellular signal-regulated kinase (ERK-1) were determined by RT-PCR.Results The mRNA expression of NF-κB and ERK-1 were significantly higher in model group (P<0.01) .Conclusion The activation of ERK signal transduction pathway enhances HSC proliferation of rats;on the other hand, the enhanced signal of nuclear factor kappaB may accelerate the procedure of liver fibrosis.Both of them may play an important role in liver fibrogenesis of rat.
- NF-κB /
- ERK-1 /
- liver fibrosis

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参考文献(8)

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