慢性乙肝病毒感染者外周血树突状细胞亚群与血清HBVDNA的关系研究

董加秀; 赵守松; 高继君; 焦贤春; 李柏青; 金齐力; 崔琢

慢性乙肝病毒感染者外周血树突状细胞亚群与血清HBVDNA的关系研究

1. 蚌埠医学院第一附属医院
2. 蚌埠医学院第二附属医院
3. 蚌埠医学院

基金项目:

安徽省教育厅自然科学研究项目(2006KJ105C)；

详细信息

中图分类号: R512.62;R450
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出版历程
- 出版日期: 2009-03-20

Sdudy the relationship between serum hepatitis B virus deoxyribonucleic acid and circulating dendritic cell subsets in patients with hepatitis B infection

Research funding:

摘要

摘要: 目的探讨慢性乙肝病毒感染者外周血树突状细胞（DCs）亚群【髓系DC（MDC）和浆细胞DC（PDC）】的变化与血清HBVDNA的关系。方法采集健康人和慢性乙肝病毒感染者外周静脉抗凝全血,利用荧光抗体标记和流式细胞仪检测外周血树突状细胞亚群（MDC的特异性标记为L ineage-HLA-DR+CD11 c+,PDC的特异性标记为L ineage-HLA-DR+CD123+）;固相放免法定量检测血清乙型肝炎病毒标志。结果慢性乙肝病毒感染者外周血PDC相对平均百分率低于健康对照者,但缺少统计学意义,MDC相对数量在两者间没有差别;血清HBeAg阳性慢性乙型肝炎患者外周血PDC相对数量显著高（P<0.01）于HBeAg阴性患者和健康人（P<0.05）,而在上述3组中MDC相对数量无差异（P=0.194）。结论HBV感染可导致患者的免疫功能改变,使机体产生免疫耐受或DC的凋亡;慢性乙型肝炎患者外周血PDC增加与HBeAg血清转换有关,提示PDC可能有抑制病毒的作用。
- 慢性肝炎,乙型 /
- 树突状细胞 /
- 亚群 /
- HBVDNA
Abstract: Objective To investigate the influence of serum HBVDNA levels on the characterizathion of circulating dendritic cell subsets in patients with chronic hepatitis B infection.Methods The plasmacytoid DC (DC2, Lineage-HLA-DR+CD123+) and non-IFN-alpha producingmyeloid DC (DC1, Lineage-HLA-DR+CD11c+) in fresh peripheral blood from chronic hepatitis B infection without any anti-viral treatment and healthy donors were enumerated by using four-color flow cytometry.The serological markers of hepatitis B virus were evaluated by solid phase radioimmunoassay (RIA) .Results Percentage of PDC in patientswith chronic hepatitis B was numerically decreased and subsequently Produce less than that of nomal controls, but without statistical difference.Further, serum HBeAg positive patients with chronic hepatitis B exhibited a significant increase in percentage of circulating PDC, compared with HBeAg negative patients and health donors (P<0.01;P<0.05, respectively) .And there is no significant difference of MDC in peripheral blood between chronic hepatitis B patients and nomal controls or among three groups above (P=0.194) .Conclusion The results suggested that the HBV infection may cause patient's immunity function change and lead to the immune tolerance or apoptosis;The percentage of peripheral PDC is associated with the HBeAg seroconversion and may plays a role on inhibition of HBV replication in patients with chronic hepatitis B.
- Hepatitis B /
- chronic /
- dendritic cells /
- Subsets

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