CA/PEI亲和膜去除肝硬化腹水中内毒素及胆红素的研究

白淑艳; 段志军; 潘明臣; 高志红

CA/PEI亲和膜去除肝硬化腹水中内毒素及胆红素的研究

基金项目:

国家自然科学基金资助项目(2005-30570515)；大连市科技资助项目(2002-B3NS137)；

详细信息

中图分类号: R575.2
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出版历程
- 出版日期: 2009-03-20

Endotoxin and bilirubin removal in ascites of patients with cirrhosis by CA/PEI affinity membrane in vitro.

Research funding:

摘要

摘要: 目的研究CA/PEI亲和膜对肝硬化腹水中内毒素及胆红素的去除效果。方法以醋酸纤维素（CA）为亲和基质,交联聚乙烯基亚胺（PEI）,制备CA/PEI亲和膜;采用动态吸附法,比较吸附前后肝硬化腹水中内毒素、胆红素、白蛋白、免疫球蛋白（IgG、IgA、IgM）及离子（K+、Na+、Cl-）浓度的变化。结果CA/PEI亲和膜吸附前后腹水中的内毒素及胆红素浓度显著降低(P（0.01）;腹水中的白蛋白、免疫球蛋白、电解质浓度过膜前后无明显变化。结论CA/PEI亲和膜能有效吸附肝硬化腹水中的内毒素及胆红素,对腹水中的白蛋白、免疫球蛋白、电解质无明显截留,可应用于肝硬化自体腹水回输治疗。
- 肝硬化腹水 /
- CA/PEI亲和膜 /
- 内毒素 /
- 胆红素
Abstract: Objective To investigate the removal of endotoxin and bilirubin from ascites of patients with cirrhosis by cellulose diacetate /polyethyleneimine affinity membrane in vitro.Methods The CA/PEI affinity membrane was prepared by employing cellulos diacetate as matrix immobilized polyethyleneimine as affinity ligand, and the dynamic absorption of ascites through CA/PEI affinity membrane was conducted.The concentration of endotoxin, bilirubin, albumin, immunoglobulin (IgG, IgA and IgM) and electrolytes (K+, Na+ and Cl-) in the ascites before and after absorption were determined.Results The endotoxin and bilirubin level were greatly reduced (P﹤0.01) after absorption.There were no markedly changes for the concentration of albumine, immunoglobulin and electrolytes in the ascites.Conclusion CA/PEI affinity membrane can effectivly absorb the endotoxin and bilirubin from the ascites, and the absorption has no obviously influence on the albumine, immunoglobulin and electrolytes at the same time.
- ascites of cirrhosis /
- cellulose diacetate /polyethyleneimine affinity membrane /
- endotoxin /
- bilirubin

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参考文献(10)

[1]张金龙, 黄志刚, 莫国生, 等.腹水浓缩超滤回输治疗肝硬化顽固性腹水42例[J].中华传染病杂志, 2003, 21 (4) ∶294-295.

[2]吕梅, 刘春英, 张伯伦.53例肝硬化难治性腹水两种疗法的临床比较[J].实用全科医学, 2007, 5 (8) ∶8-11.

[3]苏英, 杨宣, 李新月, 等.肝硬化腹水患者内毒素、细胞因子水平的检测及临床意义[J].临床肝胆病杂志, 2006, 122 (12) ∶202-203.

[4]CHEN Zhao-an, DENG Mai-cun, CHEN Yong, et al.Preparationand performance of cellulose acetate/polyethyleneimine blend micro-filtration membranes and their applications[J].Journal of MembraneScience, 2004, 235 (12) ∶73-86.

[5]贾晋斌, 谷峰, 陈尉华.内毒素受体、内毒素血症与肝硬化[J].胃肠病学, 2007, 12 (7) ∶42-43.

[6]王雷, 任成山.内毒素与慢性肝病关系的研究进展[J].中华肝脏病杂志, 2003, 11 (4) ∶251-253.

[7]张影, 张福奎, 王宝恩.细菌感染和/或内毒素血症对肝硬化患者预后的影响[J].肝脏, 2007, 12 (4) ∶310-311.

[8]WEI Gui-lin, GAO Zhi-hong, SHANG Zhen-hua, et al.Removalof Endotoxin from Human Serum Albumin Solutions by Hydrophobicand Cationic Membrane[J].Chinese Chemical Letters, 2001, 11 (4) ∶357-360.

[9] 陈兆安, 邓麦村, 叶震.膜吸附剂去除内毒素的传质研究[J].化工进展, 2003, 22∶199-201.

[10]魏桂林, 商振华, 于亿年, 等.新型亲和膜色谱用于去除胆红素的研究[J].色谱, 2001, 19 (1) :74-77.

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