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摘要: 目的探讨HBeAg阴性慢乙肝患者血清IL-27的变化规律及其临床意义。方法采用酶联免疫吸附法（ELISA）检测血清IL-27的浓度;荧光定量聚合酶链反应法（FQ-PCR）检测HBVDNA;全自动生化仪检测肝功能。结果HBeAg阳性慢乙肝组（n=38）、HBeAg阴性慢乙肝组（n=56）,血清IL-27浓度分别为（22.697±20.767）pg/ml、（34.631±22.154）pg/ml。HBeAg阴性慢乙肝组血清IL-27浓度显著高于HBeAg阳性慢乙肝组（P<0.05）。结论IL-27在慢性乙型肝炎感染机制中,可能存在一定的抗病毒作用,可能在促进HBeAg转阴以及抗-HBe的产生过程中发挥了积极作用。Abstract: Objective To investigate the change and clinical significance of serous chemokine IL-27 in patients of HBeAg negative chronic B-type hepatitis.Methods The serous concentration of IL-27 was detected by enzyme linked immunosorbent assay (ELISA) ;HBV-DNA was detected by fluorescent quantitive polymerase chain reaction (FQ-PCR) ;Liver function was assayed by automatic biochemistry analyzer.Results Concentration of serous IL-27 in HBeAg positive chronic B-type hepatitis group (n=38) , HBeAg negative chronic B-type hepatitis group (n=56) 、was (22.697±20.767) pg/ml、 (34.631±22.154) pg/ml respectively.The concentration of IL-27 in HBeAg negative chronic B-type hepatitis group was significantly higher than that of HBeAg positive chronic B-type hepatitis group, (P<0.05) .Conclusion It is possible that IL-27 promote immune clearance in the pathogenesy of chronic hepatitis B.It may affect actively on the processes of the disappearance of HBeAg and appearance of HBeAb.However, in chronic hepatitis B it may have some cytopathic effect (CPE) .
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