酒精性肝病循环抗体特异性的分析

杨永红; 骆岚; 夏培君; 张梗林

酒精性肝病循环抗体特异性的分析

山东中医药大学第二附属医院消化内科

基金项目:

铁道部济南铁路局科委基金项目(02Y41-6)；

详细信息

中图分类号: R575
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- 被引次数: 0
出版历程
- 出版日期: 2009-05-20

Research funding:

摘要

摘要: 目的探讨循环抗体在酒精性肝病患者免疫反应中的特异性。方法采用酶联免疫吸附试验（ELISA）检测40例酒精性肝病患者、40例非酒精性肝病患者、30例无肝损害的重度嗜酒者、40例健康对照的血清中循环抗体滴度。结果酒精性肝病患者抗MAAIgG滴度比健康对照组明显增加（平均OD值0.42±0.23、0.10±0.04、P<0.001）,非酒精性肝病及重度嗜酒者中抗HSA-MAAIgG抗体（平均OD值0.14±0.08、0.13±0.08）与对照组相比没有显著性差异。酒精性肝病患者抗HSA-MAAIgG值与抗HSA-乙醛（γ=0.643;P<0.0002）和抗HSA-丙醛（γ=0.773;P<0.0001）IgG值呈正相关。结论MAA加合物的循环抗体具有特异性,可引起肝脏自身免疫反应。
- MAA加合物 /
- 酒精性肝病 /
- 酶联免疫吸附试验
Abstract: Objective To explore the specificity of circulating antibodies againstmalondialdehyde-acetaldehyde adducts in the immune response associated with human alcohol-induced liver disease.Methods A MAA adduct detection assay has been developed and used to evaluate MAA antibody specific response.circulating antibodies against MAA adducts were evaluated in 40 patients with alcohol-induced liver disease (ALD) , in 40 patients with non alcohol-induced liver disease (NALD) , in 30 heavy drinkers (HD) without liver damage and in 40 healthy controls by enzyme-linked immunosorbent assays (ELISA) .Results Anti-MAA IgG titers were significantly increased in patients with ALD compared with healthy controls (mean[OD]0.42±0.23、0.10±0.04、P<0.001) , Anti-MAA IgG titers in patients with NALD (mean[OD]0.14±0.08) and HD (mean[OD]0.13±0.08) were not significantly different from those of healthy controls.The values of IgG against HAS-MAA in the patients with ALD were positively correlated with those of both anti-HAS-AcA (γ=0.643;P<0.0002) and anti-HAS-MDA (γ=0.773;P<0.0001) .Conclusions The formation of MAA antigens is specifical during alcohol-induced liver disease and suggest their possible contribution to the development of immunologic reactions.
- alcohol-induced liver disease (ALD) /
- malondialdehyde-acetaldehyde adducts (MAA) /
- enzyme-linked immunosorbent assays (ELISA)

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参考文献(14)

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