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The effect of low iron loads on Nonalcoholic Steatohepatitis in rats
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摘要: 目的研究非酒精性脂肪性肝炎(NASH)大鼠肝铁负荷的变化及低铁负荷对大鼠NASH的影响。方法SD雄性大鼠随机分为4组:正常对照组1、低铁对照组2、模型组3、低铁模型组4。实验的12周末观察血清胆固醇(TG)、甘油三酯(TC)、转氨酶(ALTAST)、血清铁(SI)、过氧化物歧化酶(SOD)、丙二醛(MDA)的含量以及肝匀浆铁、TG、TC、SOD、MDA的含量,观察肝组织病理学改变的特点。结果与正常对照组相比,模型组血清TG、TC、ALT、AST、MDA的含量明显升高,SOD的含量明显降低,血清铁含量升高不明显;肝匀浆TG、TC、ALT、AST、MDA及铁的含量明显升高,肝匀浆SOD的含量明显降低。与模型组相比,低铁模型组血清TG、TC、ALT、AST、SI、MDA含量明显降低,SOD的含量明显升高;肝匀浆TG、TC、SI、MDA含量明显降低,SOD含量明显升高。肝脏病理学切片示:模型组大鼠肝小叶内脂滴比率大于三分之一,造模成功,低铁模型组大鼠肝脏的炎症活动度计分及铁染色计分明显低于模型组。结论NASH大鼠肝脏铁负荷增加,低铁负荷可以减轻大鼠NASH的损伤程度。Abstract: Objective To study the significance of low liver iron load in the pathogenesis of Nonalcoholic steatohepatitis (NASH) in rats.Methods SD rats were randomly divided in to 4 groups:group 1 (control group) , group 2 (low liver iron load) , group 3 (model group) and group 4 (low liver iron load) .The levels of TG, TC, ALT, AST, SI, SOD and MDA in the serum and liver were measured after 12 weeks.Liver histopathologic change was observed.Results The serum levels of TG, TC, ALT, AST and MDA in group 3 were significantly higher than the control group.The serum SOD in group 3 was significantly lower than the control.There was no significant difference in SI between group 3 and the control group.The liver TG, TC, MDA and iron content in group 3 were significantly higher, and SOD was significantly lower than the control group.The serum TG, TC, ALT, AST, MDA and SI in group 4 were significantly lower and serum SOD was significantly higher than those in group 3.The TG, TC, MDA and iron content in the liver were significantly lower than those in group 3.The SOD in the liver of group 4 was significantly higher than those in group 3.The rate of lipid droplet in group 3 was higher.The liver inflammation scores and the iron load in group 4 were significantly lower than those in group 3.Conclusion Iron content highly increases in the liver of NASH rats.Low iron level can lessen the liver damage.
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Key words:
- liver ferrum /
- NASH
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