HBx、Twist、E-cadherin及N-cadherin在肝细胞肝癌中的表达及意义

滕菁; 唐南洪; 徐振兴; 李秀金; 金福顺; 王晓茜; 吴健朗

HBx、Twist、E-cadherin及N-cadherin在肝细胞肝癌中的表达及意义

1. 厦门市中医院检验科
2. 福建医科大学附属协和医院省肝胆外科研究所

基金项目:

厦门市科学技术局科技计划资助项目(3502Z20084021)；

详细信息

中图分类号: R735.7
计量
- 文章访问数: 335
- HTML全文浏览量: 24
- PDF下载量: 451
- 被引次数: 0
出版历程
- 出版日期: 2009-06-20

Expression and significance of HBx, Twist, E-cadherin and N-cadherin in hepatocellular carcinoma

Research funding:

摘要

摘要: 目的探讨HBx、Twist、E-cadherin及N-cadherin在肝细胞肝癌中的表达及意义。方法采用免疫组织化学法检测72例HBsAg阳性的肝细胞肝癌组织及癌旁组织中HBx、Twist、E-cadherin及N-cadherin的表达,并与临床病理资料做对照分析。结果HCC组织中HBx、Twist、E-cadherin及N-cadherin的阳性表达率分别为84.7%、80.6%、52.8%和45.8%,而癌旁组织的阳性表达率分别为81.9%、51.4%、70.8%和27.8%。除HBx外,后三种蛋白在肝癌组织及癌旁组织的表达差异均有显著性（P<0.05）。HBx、Twist和N-cadherin的表达升高及E-cadherin表达下降分别与有无门脉癌栓和肝外是否转移相关（P<0.05）;HBx的表达分别与Twist和N-cadherin的表达呈正相关（P<0.05）;HBx、Twist和N-cadherin的表达则分别与E-cadherin的表达呈负相关（P<0.05）。结论HBx、Twist和N-cadherin的高表达以及E-cadherin的低表达...
- 肝细胞肝癌 /
- 乙肝病毒X蛋白 /
- 转录因子Twist /
- 上皮性钙粘蛋白 /
- 神经性钙粘蛋白
Abstract: Objective To investigate the expression and clinical significance of HBx, Twist, E-cadherin and N-cadherin in hepatocellular carcinoma (HCC) .Methods The expression of HBx, Twist, E-cadherin and N-cadherin in HCC and adjacent tissues were detected by immunohistochemistry technique and the relationship with clinicopathological features was analyzed.Results The positivity rate of HBx, Twist, E-cadherin and N-cadherin were 84.7%, 80.6%, 52.8% and 45.8% in HCC tissues and 81.9%, 51.4%, 70.8% and 27.8% in adjacent tissues, respectively.There was a statistically significant difference in Twist, E-cadherin and N-cadherin between the HCC and adjacent tissues (p<0.05) .The expression of HBx was positively correlated with Twist and N-cadherin in HCC and adjacent tissues, respectively.E-cadherin was negatively correlated with HBx, Twist and N-cadherin.Conclusion The higher expression of HBx, Twist, N-cadherin and E-cadherin are correlated with the invasion and metastasis of HCC.HBx might promote the change of epithelial-mesenchymal transition of HCC through Twist upregulation.
- hepatocellular carcinoma /
- HBx /
- Twist /
- E-cadherin /
- N-cadherin

HTML全文

参考文献(15)

[1]Xu Z, Shen MX, Ma DZ, et al.TGF-beta1-promoted epithelial-to-mesenchymal transformation and cell adhesion contribute to TGF-beta1-enhanced cell migration in SMMC-7721cells[J].Cell Res, 2003, 13 (5) ∶343-350.

[2] 王妍, 薛同春, 谢晓莺, 等.上皮间质转化与肝细胞癌肺转移的相关性研究[J].中华外科杂志, 2008, 46 (21) ∶1624-7.

[3]Shijubo N, Uede T, Kon S, et al.Vascular endothelial growth factor and osteopontin in stage1lung adenocarcinoma[J].Am J Respir Crit Care Med, 1999, 160 (4) ∶1269-1273

[4]Greenburg G, Hay ED.Epithelia suspended in collagen gels can lose polarity and express characteristics of migrating mesenchymal cells[J].J Cell Biol, 1982, 95 (1) ∶333-339.

[5]Cavallaro U, Christofori G.Cell adhesion and signalling by cadherins and Ig-CAMs in cancer[J].Nat Rev Cancer, 2004, 4∶118-132.

[6]Lopez D, Niu G, Huber P, et al.Tumor-induced upregulation of Twist, Snail, and Slug represses the activity of the human VE-cad-herin promoter[J].Arch Biochem Biophys, 2009, 482 (1-2) ∶77-82.

[7]Yang J, Mani SA, Donaher JL, et al.Twist, a master regulator of morphogenesis, plays an essential role in tumor metastasis[J].Cell, 2004, 117 (7) ∶927-939.

[8]Lee TK, Poon RT, Yuen AP, et al.Twist expression correlates with hepatocellular carcinoma metastasis through induction of epithelial-mesenchymal transition[J].Clin Cancer Res, 2006, 12 (18) ∶5369-5376.

[9]李常海, 陈孝平, 徐宗全, 等.Twist基因在肝细胞肝癌中的表达及意义[J].中华外科杂志, 2006, 44 (19) ∶1353-1356.

[10]Nagi C, Guttman M, Jaffer S, et al.N-cadherin expression in breast cancer∶correlation with an aggressive histologic variant--in-vasive micropapillary carcinoma[J].Breast Cancer Res Treat, 2005, 94 (3) ∶225-235.

[11]Hazan RB, Qiao R, Keren R, et al.Cadherin switch in tumor pro-gression[J].Ann NY Acad Sci, 2004, 1014 (2) ∶155-163.

[12]Chan CF, Yau TO, Jin DY, et al.Evaluation of nuclear factor-kappaB, urokinase-type plasminogen activator, and HBx and their clinicopathological significance in hepatocellular carcinoma[J].Clin Cancer Res, 2004, 10 (12) ∶4140-4149.

[13]Lara-Pezzi E, Gmez-Gaviro MV, Glvez BG, et al.The hepa-titis B virus X protein promotes tumor cell invasion by inducing mem-brane-type matrix metalloproteinase-1and cyclooxygenase-2ex-pression[J].J Clin Invest, 2002, 110 (12) ∶1831-1838.

[14]Lee TK, Man K, Poon RT, et al.Signal transducers and activators of transcription5b activation enhances hepatocellular carcinoma ag-gressiveness through induction of epithelial-mesenchymal transition[J].Cancer Res, 2006, 66 (20) ∶9948-9956.

[15]Lara-Pezzi E, Roche S, Andrisani OM, et al.The hepatitis B vi-rus HBx protein induces adherens junction disruption in a src-de-pendent manner[J].Oncogene, 2001, 20 (26) ∶3323-3331.

施引文献

资源附件(0)

访问统计