PDF下载 ( 2499 KB)
Detection and dynamic changes of serum Th1/Th2 related cytokines in chronic hepatitis C virus infected patients
-
摘要:
目的了解慢性HCV感染与Th1/Th2相关细胞因子的相互关系及机制。方法使用ELISA方法检测64份慢性HCV感染者血清中IL-2,IFN-γ,TNF-α,IL-4,IL-10。结果肝炎和肝硬化患者中IL-2,IFN-γ无明显差别;肝硬化患者中IL-4,IL-10高于肝炎患者;而TNF-α相反。对6例慢性HCV感染者血清进行动态检测,发现IL-4,IL-10有逐渐增高的趋势;IL-2,TNF-α,IFN-γ有下降趋势。慢性肝炎急性发作时,IL-2,TNF-α,IFN-γ有所增高。结论慢性HCV感染者外周血中存在Th1/Th2细胞失平衡,以Th2型免疫应答为主。Th1/Th2细胞失平衡在慢性HCV感染发展过程中起重要作用。
-
[1]Cecere A, Marotta F, Vangieri B, et al.Progressive liver injury in chronic hepatitis C infection is related to altered cellular immune re-sponse and to different citokine profile[J].panminerva med2004, 46∶171-187. [2]Gajewski TF, Fitch FW.Anti-proliferative effect of IFN-gamma in immune regulation.I.IFN-gamma inhibits the proliferation of Th2but not Th1murine helper T lymphocyte clones[J].J Immunol, 1988, 140 (12) ∶4245-4252. [3]Tsai SL, Liaw YF, Chen MH, et al.Detection of type2-like T-helper cells in hepatic C virus infection:implications for hepatitis C virus chronicity[J].Hepatology, 1997, 25∶449-458. [4]Modlin RL, Nutman TB.Type2cytokines and negative immune regu-lation in human infections[J].Curr Opin Immunol, 1993, 5 (4) ∶511-517. [5]Kantzanou M, Lucas M, Barnes E, et al.Viral escape and T cell ex-haustion in hepatitis C virus infection analysed using Class I peptide tetramers[J].Immunol Lett.2003, 85 (2) ∶165-171. [6]Mihm S, Schweyer S, Ramadori G.Expression of the chemokine IP-10correlates with the accumulation of hepatic IFN-gamma and IL-18mRNA in chronic hepatitis C but not in hepatitis B[J].J Med Virol, 2003, 70 (4) ∶562-570. [7]Yoshioka M, Ito T, Miyazaki S, et al.The release of tumor necrosis factor-alpha, interleukin-1, interleukin-6and prostaglandin E2in bovine Kupffer cells stimulated with bacterial lipopolysaccharide[J].Vet Immunol Immunopathol, 1998, 66 (3-4) ∶301-307. [8]Lee CH, Choi YH, Yang SH, et al.Hepatitis C virus core protein in-hibits interleukin12and nitric oxide production from activated macro-phages[J].Virology, 2001, 279 (1) ∶271-279. [9]Dolganiuc A, Chang S, Kodys K, et al.Hepatitis C virus (HCV) core protein-induced, monocyte-mediated mechanisms of reduced IFN-alpha and plasmacytoid dendritic cell loss in chronic HCV in-fection[J].J Immunol, 2006, 177 (10) ∶6758-6768. [10]Cook DN, Prosser DM, Forster R, et al.CCR6mediates dendritic cell localization, lymphocyte homeostasis, and immune responses in mucosal tissue[J].Immunity, 2000, 12 (5) ∶495-503. [11]Katayama K, Kasahara A, Sasaki Y, et al.Immunological response to interferon-gamma priming prior to interferon-alpha treatment in refractory chronic hepatitis C in relation to viral clearance[J].J Vi-ral Hepat, 2001, 8 (3) ∶180-185. [12]Schlaak JF, Schramm C, Radecke K, et al.Sustained suppression of HCV replication and inflammatory activity after interleukin-2therapy in patients with HIV/hepatitis C virus coinfection[J].J Ac-quir Immune Defic Syndr, 2002, 29 (2) ∶145-148. [13]Barth H, Klein R, Berg PA, et al.Analysis of the effect of IL-12therapy on immunoregulatory T-cell subsets in patients with chronic hepatitis C infection[J].Hepatogastroenterology, 2003, 50 (49) ∶201-206. [14]Asadullah K, Sterry W, Volk HD.Interleukin-10therapy--re-view of a new approach[J].Pharmacol Rev, 2003, 55 (2) ∶241-269. [15]Louis H, Le Moine O, Goldman M, et al.Modulation of liver inju-ry by interleukin-10[J].Acta Gastroenterol Belg, 2003, 66 (1) ∶7-14. -
本文二维码
计量
- 文章访问数: 3039
- HTML全文浏览量: 3
- PDF下载量: 634
- 被引次数: 0
下载:
