DC-SIGN在慢性乙型肝炎病毒感染时树突状细胞成熟活化中的作用

邹小静; 姜雪强; 田德英

DC-SIGN在慢性乙型肝炎病毒感染时树突状细胞成熟活化中的作用

华中科技大学同济医学院附属同济医院感染科

基金项目:

湖北省自然科学基金项目(2008CDA049)；

详细信息

中图分类号: R512.62
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出版历程
- 出版日期: 2011-02-20

Role of DC-SIGN on the maturation and activation of dendritic cells in chronic hepatitis B virus infection

Research funding:

摘要

摘要: 目的研究慢性HBV感染时,DC-SIGN在树突状细胞（DC）成熟和活化中介导的作用。方法将α-甘露糖苷酶抑制剂-基夫碱作用于HepG2.2.15细胞,收获上清中的高甘露糖型HBV颗粒,并于第5 d加入到外周血单核细胞衍生的DC培养基中,培养至第7 d,采用流式细胞仪检测DC表面CDla、CD83、CD80、CD86、HLA-DR分子的表达,MTT法检测DC刺激淋巴细胞增殖的能力,ELISA法检测DC分泌IL-12的水平。结果高甘露糖型HBV组,与天然的HBV组相比,DC表面CDla、CD83、CD80、CD86、HLA-DR分子的表达增加,分泌IL-12的水平升高,刺激同种异体淋巴细胞增殖的能力亦明显增强,且上述效应均可被DC-SIGN特异性抗体所阻断。结论 DC-SIGN识别高甘露糖型HBV后可以促进DC的成熟和活化,天然的HBV可能利用α-甘露糖苷酶参与的去甘露聚糖修饰来逃避DC-SIGN的识别,从而诱导DC功能的缺陷。
- 肝炎,乙型,慢性 /
- 肝炎病毒,乙型 /
- 树突细胞
Abstract: Objective To investigate the role of DC-SIGN on the maturation and activation of dendritic cells (DC) in chronic HBV infection.Methods Highly mannosylated HBV obtained by treating HepG2.2.15 cells with the a-mannosidase I inhibitor kifunensine were added to the medium of DC derived from peripheral blood mononuclear cells on day 5 after culture.The expression rates of CDla, CD83, CD80, CD86 and HLA-DR of DCs were assayed by flow cytometry analysis, the stimulating capacity of DC was detected by MTT assay and the levels of IL-12 released by DCs were measured by ELISA on day 7.Results Compared with native HBV group, the expression of CDla, CD83, CD80, CD86 and HLA-DR of DC were significantly improved, levels of IL-12 were increased, and capacity of stimulating lymphocyte proliferation was enhanced in highly mannosylated HBV group.Furthermore these effects could be all blocked by DC-SIGN specific antibody.Conclusion DC-SIGN can promote the maturation and activation of DC after recognizing highly mannosylated HBV, native HBV may exploit demannosylation as a way to escape recognition by DC-SIGN and thereby induce DC dysfunction.
- hepatitis B /
- chronic /
- hepatitis B virus

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参考文献(12)

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