β连环蛋白在大鼠肝纤维化模型中的动态表达及意义

叶超; 陈永平; 戴志娟; 李文渊; 林镯; 程瑗; 金晓芝

β连环蛋白在大鼠肝纤维化模型中的动态表达及意义

温州医学院附属第一医院感染科

基金项目:

中国肝炎防治基金会王宝恩肝纤维化研究基金(20080017)；

详细信息

中图分类号: R575.2
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出版历程
- 出版日期: 2011-03-20

Dynamic expression of β-catenin in rat models of hepatic fibrosis and its significance

Research funding:

摘要

摘要: 目的研究wnt信号通路的核心因子β连环蛋白（β-catenin）在肝纤维化大鼠模型中的动态表达及意义。方法腹腔注射二甲基亚硝胺（DMN）诱导大鼠肝纤维化模型。分别在造模后4 d、1、2、4、6、8周等不同时间点测血清ALT、AST、TBil、Alb的变化,采用HE及Masson染色观察肝组织学变化,反转录聚合酶链反应（RT-PCR）检测不同时间点肝脏β-catenin mRNA的表达。结果正常肝组织中有少量β-catenin表达,随着肝纤维化的发展,β-catenin的表达量逐渐增加,至第4周达到高峰,第8周略有下降,但仍高于正常。β-catenin的表达量与肝脏纤维化分期（S0～S4）呈正相关（rs=0.926,P<0.001）。结论β-catenin可能参与肝纤维化的发生,并与纤维化的发展过程密切相关。
- β连环素 /
- 肝硬化,实验性 /
- 信号传导 /
- 基因表达
Abstract: Objective To explore the expression and significance of β-catenin (the core factor of wnt signaling pathway) in rat models of hepatic fibrosis.Methods The hepatic fibrosis of rat was induced by intraperitoneal injection of DMN.The changes in serum ALT, AST, TBill and ALB were tested on day 4 and 1, 2, 4, 6, 8 weeks after injection.The Histopathologic changes of the liver were observed with HE and MASSON stain.The β-catenin mRNA was detected by RT-PCR dynamically at 4 days, 1, 2, 4, 6 and 8 weeks after injection.Results β-catenin mRNA could be detected in normal rat liver.But with the development of hepatic fibrosis, β-catenin mRNA expression level increased gradually.The expression reached its peak at 4 weeks after injection, and decreased at 8 weeks after injection, but remained relatively high level above normal range.And the expression of β-catenin mRNA was positively correlated with the hepatic fibrosis stages (rs=0.926, P<0.001) .Conclusion β-catenin may involve in the genesis of hepatic fibrosis and closely related to the development of liver fibrosis.
- beta catenin /
- liver cirrhosis /
- experimental /
- signal transduction

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参考文献(11)

[1]Moon RT, Kohn AD, De Ferrari GV, et al.WNT and beta-catenin signalling:diseases and therapies[J].Nat Rev Genet, 2004, 5 (9) :691-701.

[2]Konigshoff M, Balsara N, Pfaff EM, et al.Functional Wnt signaling is increased in idiopathic pulmonary fibrosis[J].PLoS One, 2008, 3 (5) :e2142.

[3]Bayle J, Fitch J, Jacobsen K, et al.Increased expression of Wnt2 and SFRP4 in Tsk mouse skin:role of Wnt signaling in altered dermal fibrillin deposition and systemic sclerosis[J].J Invest Dermatol, 2008, 128 (4) :871-881.

[4]中华医学会.病毒性肝炎防治方案[J].中华肝脏病杂志, 2000, 8 (6) :324-329.

[5]Thompson MD, Monga SP.WNT/beta-catenin signaling in liver health and disease[J].Hepatology, 2007, 45 (5) :1298-1305.

[6]Shackel NA, McGuinness PH, Abbott CA, et al.Identification of novel molecules and pathogenic pathways in primary biliary cirrhosis:cDNA array analysis of intrahepatic differential gene expression[J].Gut, 2001, 49 (4) :565-576.

[7]Tien LT, Ito M, Nakao M, et al.Expression of beta-catenin in hepatocellular carcinoma[J].World J Gastroenterol, 2005, 11 (6) :2398-2401.

[8]Tsukada S, Parsons CJ, Rippe RA.Mechanisms of liver fibrosis[J].Clin Chim Acta, 2006, 364 (1-2) :33-60.

[9]Myung SJ, Yoon JH, Gwak GY, et al.Wnt signaling enhances the activation and survival of human hepatic stellate cells[J].FEBS Lett, 2007, 581 (16) :2954-2958.

[10]Tsukamoto H, She H, Hazra S, et al.Anti-adipog-enic regulation underlies hepatic stellate cell transdifferentiation[J].J Gastroenterol Hepatol, 2006, 21 (Suppl 3) :S102-105.

[11]黎仕锋, 乐亮, 杨小晓, 等.wnt信号通路在肝纤维化中的作用[J].中山大学研究生学刊, 2010, 31 (1) :10-14.

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