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The study of the expression of COX-2 and CDKN2A in chronic hepatitis B and liver cirrhosis
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摘要: 目的了解慢性乙型肝炎（CHB）、肝硬化肝组织中COX-2、CDKN2A的表达及其与肝脏损伤程度的关系。方法经免疫学及肝穿刺病理学证实为CHB患者46例、乙型肝炎肝硬化17例;正常肝组织10例（来自肝脏良性肿瘤手术切除的标本）。应用病理学判断肝组织的炎症及纤维化程度。采用免疫组化方法、半定量逆转录聚合酶链反应（RT-PCR）和免疫印迹分析法检测COX-2、CDKN2A在组织中的表达。结果 （1）CDKN2A mRNA第一、第二外显子在正常肝、CHB及肝硬化组织的表达水平及CDKN2A蛋白的半定量分析差异有统计学意义（F=28.75、24.86、26.43,P=0.036、0.041、0.038）。（2）正常肝、CHB及肝硬化组织中的COX-2、CDKN2A蛋白水平的表达差异有统计学意义（χ2=13.48、15.73,P=0.042、0.021）;肝组织炎症不同级别的COX-2、CDKN2A蛋白水平的表达差异具有统计学意义（χ2=22.45、23.67,P=0.038、0.027）;肝组织纤维化不同程度的COX-2、CDKN2A蛋白水平的表达差异具有统计学意义（χ2=24.16、22.53...
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关键词:
- 环氧化酶2 /
- 周期素依赖激酶抑制剂p16 /
- 肝炎,乙型,慢性 /
- 肝硬化 /
- 基因表达
Abstract: Objective To investigate the expression of cyclooxygenase-2 (COX-2) and CDKN2A in chronic hepatitis B and liver cirrhosis tissues and the relationship between the expression and the liver injury.Methods There were 46 patients with chronic hepatitis B and 17 cases with liver cirrhosis were confirmed through the immunology of liver biopsy;There were 10 normal liver tissues from benign tumors of the liver specimens.Liver inflammation and fibrosis was determined by Pathology.The expression of COX-2 and CDKN2A was determined by immunohisto-chemistry, semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blot.Results (1) The expression level of the first and second exon of CDKN2A mRNA and the semiquantitative analysis of CDKN2A protein in the normal liver、hepatitis B and cirrhosis were significant (F=28.75, 24.86, 26.43, P=0.036, 0.041, 0.038) . (2) The protein level's expression of COX-2, CDKN2A in normal liver, hepatitis B, cirrhosis was significantly different (χ2=13.48, 15.73, P= 0.042, 0.021) ;The protein level's expression of COX-2, CDKN2A in liver inflammation with different levels was significantly different (χ2=22.45, 23.67, P=0.038, 0.027) ;The protein level's expression of COX-2, CDKN2A in Liver fibrosis with different degrees was significantly different (χ2=24.16, 22.53, P=0.024, 0.037) .Conclusion The expression of COX-2 and CDKN2A was related to the development of process of hepatitis and cirrhosis.The COX-2 and CDKN2A may be used as a good indicator of HBV-related liver disease progress.-
Key words:
- cyclooxygenase 2 /
- cyclin-dependent kinase inhibitor p16 /
- hepatitis B /
- chronic /
- liver cirrhosis
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