HBV基因型与HBeAg的表达关系及其临床意义

汪莉萍; 韩方正; 吴光辉

HBV基因型与HBeAg的表达关系及其临床意义

徐州医学院传染病学教研室

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中图分类号: R512.62
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出版历程
- 出版日期: 2011-05-20

Relationship between the genotypes of hepatitis B virus and the expression of HBeAg and its clinical significance

摘要

摘要: 目的观察慢性乙型肝炎病毒（HBV）感染者HBV基因型与HBeAg表达和病情轻重的关系。方法利用型特异性引物多重PCR方法检测HBV基因型,时间分辨荧光法检测HBV DNA。结果在120例慢性乙型肝炎病毒感染者中HBV基因型C型84例（70%）、B型31例（25.8%）,BC混合型5例（4.2%）,未发现A、D、E、F基因型;C型在慢性重型肝炎组最高（P<0.05）;在C基因型中HBeAg（+）患者较HBeAg（-）患者多见（P<0.05）,在B基因型中,HBeAg（+）和HBeAg（-）患者分布无明显差别。结论徐州地区HBV基因型以C型和B型多见,e抗原的表达率在C型中较高;基因型B型与C型相比,C型引起肝脏损伤重。
- 肝炎病毒,乙型 /
- 肝炎e抗原,乙型 /
- 基因型 /
- 基因表达
Abstract: Objective To investigate the relationship between different genotypes of hepatitis B virus (HBV) and the expression of HBeAg and the severity of liver diseases.Methods The HBV genotypes were determined by multiple-primer PCR amplification and the HBV DNA levels were detected by fluorescent quantitative PCR assay.Results In 120 HBV infected cases, 84 cases (70%) were genotype C, 31 cases (25.8%) were genotype B and 5 cases (4.2%) were mixed genotype BC;genotypes A, D, E, F were not detected.More patients were HBeAg-positive than were HBeAg-negative in Genotype C (P<0.05) .There was no significant difference between the HBeAg negative and HBeAg positive in genotyoe B.Conclusion This study suggests that genotype B and C are predominant genotype in Xuzhou area.The HBeAg positive expression is predominant in genotype C.Compare to the patients with genotype B, more severe inflammation was observed in patients with genotype C.
- hepatitis B virus /
- hepatitis B e antigens /
- genotype /
- gene expression

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参考文献(13)

[1]许军, 王齐欣, 蒋栋, 等.乙型肝炎病毒基因型与病情轻重的关系[J].中华肝脏病杂志, 2003, 11 (1) :11-13.

[2]温志立, 谭德明, 侯周华, 等.型特异性引物PCR研究HBV基因型与临床表现的关系[J].中南大学学报 (医学版) , 2004, 29 (1) :50-53.

[3]中华医学会.病毒性肝炎防治方案[J].中华肝脏病杂志, 2000, 8 (6) :324-329.

[4]Naito H, Hayashi S, Abe K.Rapid and specific genotypingsystem for hepatitis B virus corresponding to six majorgenotypes by PCR with type-specific primers[J].J ClinMicrobiol, 2001, 39 (1) :362-364.

[5]Kuang SY, Jackson PE, Wang JB, et al.Specific mutations ofhepatitis B virus in plasma predict liver cancer development[J].Proc Natl Acad Sci U S A, 2004, 101 (10) :3575-3580.

[6]Yotsuyanagi H, Hino K, Tomita E, et al.Precore and corepromoter mutations, hepatitis B virus DNA levels andprogressive liver injury in chronic hepatitis B[J].J Hepatol, 2002, 37 (3) :355-363.

[7]Chan HL, Leung NW, Hussain M, et al.Hepatitis B e antigennegative chronic hepatitis B in HongKong[J].Hepatology, 2003, 31 (3) :763-768.

[8]梁蔚芳, 河海棠, 刘志华, 等.乙型肝炎病毒前C/C区及其调控基因变异的研究[J].解放军医学杂志, 2005, 30 (4) :331-334.

[9]Liu CJ, Chen PJ, LaiMY, et al.Evolution of precore/corepromoter mutations in hepatitis B carriers with hepatitis B eantigen seroreversion[J].J Med Virol, 2004, 74 (2) :237-245.

[10]Chun YK, Kim JY, Woo HJ, et al.No significant correlationexists between core promoter mutations, viral replication, and liver damage in chronic hepatitis B infection[J].Hepatology, 2000, 32 (5) :1154-1162.

[11]Kao JH, Chen PJ, Lai MY, et al.Basal core promoter mutations of hepatitis B virus increase the risk of hepatocellularcarcinoma in hepatitis B carriers[J].Gastroenterology, 2003, 124 (2) :327-334.

[12]Du H, Li T, Zhang HY, et al.Correlation of hepatitis B virus (HBV) genotypes and mutations in basal core promoter/precore with clinical features of chronic HBV infection[J].Liver Int, 2007, 27 (2) :240-246.

[13]Kawabe N, Hashimoto S, Harata M, et al.The loss of HBeAgwithout precore mutation results in lower HBV DNA levelsand ALT levels in chronic hepatitis B virus infection[J].Gastroenterol, 2009, 44 (7) :751-756.

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