兰州地区丙型肝炎病毒基因变异与干扰素α应答关系的探讨

张文杰; 杜绍财; 饶慧瑛; 李朝霞; 田淑菊; 毛维武; 龚晏萱; 屈延

兰州地区丙型肝炎病毒基因变异与干扰素α应答关系的探讨

1. 甘肃省第二人民医院感染科
2. 北京大学人民医院北京大学肝病研究所

基金项目:

甘肃省科学技术厅自然科学基金项目(0710RJZA005)；

详细信息

中图分类号: R512.63
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出版历程
- 出版日期: 2011-05-20

Study on the HCV gene mutation in Lanzhou and the relationship with interferon-α induced immune response

Research funding:

摘要

摘要: 目的探讨兰州地区丙型肝炎病毒（HCV）1b基因型5’非编码区（5’NCR）基因变异株的感染状态,及其与干扰素α疗效的关系。方法应用限制性内切酶酶切分析检测40例HCV 1b型中5’-NCR基因变异及9例干扰素α治疗患者中的5’-NCR基因变异。结果在40例HCV 1b型中存在5种感染状态:（1）有MboⅠ切点24/40（60.0%）;（2）无MboⅠ切点变异株5/40（12.5%）;（3）有MboⅠ和无MboⅠ切点混合感染株7/40（17.5%）;（4）有BamHⅠ切点变异株2/40（5%）;（5）有MboⅠ双切点变异株2/40（5%）。对9例干扰素α治疗的丙型肝炎患者血清进行5’-NCR变异株检测。5例干扰素α应答病例中,2例为2a型,3例为无MboⅠ切点的1b型。4例抗干扰素α病例中,1例为2a型,但在某节段存在着1b与2a混合状态,另3例为无MboⅠ。结论在丙型肝炎患者血清中存在单一的MboⅠ切点的样品,24/40（60.0%）可能是HCV野生株感染的状态。
- 肝炎病毒属 /
- 干扰素α /
- 变异(遗传学)
Abstract: Objective To study the relationship between gene mutation of 5' noncoding region (5'NCR) of hepatitis C virus (HCV) genotype 1b in infection state and effect of interferon-alpha (IFNα) on the treatment of chronic hepatitis C.Methods Sera from 9 patients were collected before, during and after IFNα therapy and sera from 40 HCV 1b patients were collected.HCV 5'NCR fragments were amplified from these samples by RT-PCR and sequenced by cutting with restriction endonuclease Mbo I and BamH I.Results There were 5 infection states in 40 patients with HCV 1b: (1) Mbo I restriction site was detected in 24 patients (24/40, 60.0%) ; (2) Mutation without Mbo I restriction site was detected in 5 patients (5/40, 12.5%) ; (3) 7 patients (7/40, 17.5%) coinfected the strain with or without of Mbo I restriction site; (4) Mutation with BamH I restriction site was detected in 2 patients (2/40, 0.5%) ; (5) 2 patients (2/40, 0.5%) presented with two Mbo I restriction sites.Then we checked the mutation of 5'noncoding region of the sera from 9 patients with IFNα therapy.In the 5 patients with sustained virological response (SVR) , 2 for HCV genotype 2a, 3 for HCV genotype 1b without Mbo I restriction site.In the 4 patietns with nonresponder, 1 for coinfection of HCV genotype 1b and 2a, 3 for HCV genotype 1b without Mbo I restriction site.Conclusion Of 40 HCV genotype 1b serum samples, 60.0% patients presented single Mbo I restriction site, suggesting the state of infection with wild type strain.
- hepacivirus /
- interferon-alpha /
- variation (genetics)

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参考文献(8)

[1]Ghany MG, Strader DB, Thomas DL, et al.Diagnosis, management, and treatment of hepatitis C:an update[J].Hepatology, 2009, 49 (4) :1335-1374.

[2]Dienstag JL, McHutchison JG.American Gastroenterological Association Technical Review on the Management of Hepatitis C[J].Gastroenterology, 2006, 130 (1) :231-264.

[3]马慧, 王豪.长效干扰素-α:聚乙二醇干扰素-α研究进展[J].中华肝脏病杂志, 2002, 10 (1) :78-79.

[4]于建武, 孙丽杰, 李晓光, 等.快速和早期病毒学应答对慢性丙型肝炎患者持续病毒学应答的预测价值[J].中华传染病杂志, 2008, 26 (1) :36-39.

[5]孙南雄, 范晓峰, 杜绍财, 等.丙型肝炎病毒Simmonds基因分型法酶切分型研究[J].江苏医药, 1999, 25 (7) :481-483.

[6]刘丽君, 张瑞, 李俊强, 等.中国南部和西部地区HCV感染的基因型分布特征[J].中国现代医学, 2007, 17 (19) :2337-2340.

[7]杜绍财, 邱国华, 刘峰, 等.中国丙型肝炎病毒1b型5'NCR变异研究[J].中华检验医学杂志, 2005, 28 (2) :162-168.

[8] 邱国华, 吴娟, 刘丽君, 等.HCV 5'NCR基因变异与干扰素-α免疫应答关系的探讨[J].现代检验医学, 2009, 24 (1) :118-120.

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