兰州地区丙型肝炎病毒基因变异与干扰素α应答关系的探讨

张文杰; 杜绍财; 饶慧瑛; 李朝霞; 田淑菊; 毛维武; 龚晏萱; 屈延

兰州地区丙型肝炎病毒基因变异与干扰素α应答关系的探讨

1. 甘肃省第二人民医院感染科
2. 北京大学人民医院北京大学肝病研究所

基金项目:

甘肃省科学技术厅自然科学基金项目(0710RJZA005)；

详细信息

中图分类号: R512.63
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出版历程
- 出版日期: 2011-05-20

Study on the HCV gene mutation in Lanzhou and the relationship with interferon-α induced immune response

Research funding:

摘要

摘要: 目的探讨兰州地区丙型肝炎病毒（HCV）1b基因型5’非编码区（5’NCR）基因变异株的感染状态,及其与干扰素α疗效的关系。方法应用限制性内切酶酶切分析检测40例HCV 1b型中5’-NCR基因变异及9例干扰素α治疗患者中的5’-NCR基因变异。结果在40例HCV 1b型中存在5种感染状态:（1）有MboⅠ切点24/40（60.0%）;（2）无MboⅠ切点变异株5/40（12.5%）;（3）有MboⅠ和无MboⅠ切点混合感染株7/40（17.5%）;（4）有BamHⅠ切点变异株2/40（5%）;（5）有MboⅠ双切点变异株2/40（5%）。对9例干扰素α治疗的丙型肝炎患者血清进行5’-NCR变异株检测。5例干扰素α应答病例中,2例为2a型,3例为无MboⅠ切点的1b型。4例抗干扰素α病例中,1例为2a型,但在某节段存在着1b与2a混合状态,另3例为无MboⅠ。结论在丙型肝炎患者血清中存在单一的MboⅠ切点的样品,24/40（60.0%）可能是HCV野生株感染的状态。
- 肝炎病毒属 /
- 干扰素α /
- 变异(遗传学)
Abstract: Objective To study the relationship between gene mutation of 5' noncoding region (5'NCR) of hepatitis C virus (HCV) genotype 1b in infection state and effect of interferon-alpha (IFNα) on the treatment of chronic hepatitis C.Methods Sera from 9 patients were collected before, during and after IFNα therapy and sera from 40 HCV 1b patients were collected.HCV 5'NCR fragments were amplified from these samples by RT-PCR and sequenced by cutting with restriction endonuclease Mbo I and BamH I.Results There were 5 infection states in 40 patients with HCV 1b: (1) Mbo I restriction site was detected in 24 patients (24/40, 60.0%) ; (2) Mutation without Mbo I restriction site was detected in 5 patients (5/40, 12.5%) ; (3) 7 patients (7/40, 17.5%) coinfected the strain with or without of Mbo I restriction site; (4) Mutation with BamH I restriction site was detected in 2 patients (2/40, 0.5%) ; (5) 2 patients (2/40, 0.5%) presented with two Mbo I restriction sites.Then we checked the mutation of 5'noncoding region of the sera from 9 patients with IFNα therapy.In the 5 patients with sustained virological response (SVR) , 2 for HCV genotype 2a, 3 for HCV genotype 1b without Mbo I restriction site.In the 4 patietns with nonresponder, 1 for coinfection of HCV genotype 1b and 2a, 3 for HCV genotype 1b without Mbo I restriction site.Conclusion Of 40 HCV genotype 1b serum samples, 60.0% patients presented single Mbo I restriction site, suggesting the state of infection with wild type strain.
- hepacivirus /
- interferon-alpha /
- variation (genetics)

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参考文献(8)

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