HMGB1在肝癌及癌旁肝组织中的表达及意义

高保华; 汪雯; 牛春燕; 郑建云; 刘冰; 罗建梅

HMGB1在肝癌及癌旁肝组织中的表达及意义

1. 西安医学院附属医院消化内科
2. 西安医学院附属医院病理科
3. 西安医学院附属医院超声科

详细信息

中图分类号: R735.7
计量
- 文章访问数: 3592
- HTML全文浏览量: 15
- PDF下载量: 753
- 被引次数: 0
出版历程
- 出版日期: 2011-08-20

The expression of HMGB1 in hepatocellular carcinoma, paracancerous liver tissue and its significance

摘要

摘要: 目的了解高迁移率族蛋白B1(HMGB1)在肝炎、肝癌癌旁组织、肝癌组织中的表达,及其与肝组织损伤程度、肝癌发生发展的关系。方法慢性乙型肝炎(CHB)患者46例,肝癌癌旁组织标本20例,肝癌组织标本20例(配对组织),采用常规HE染色法观察肝组织炎症与纤维化程度,用免疫组化方法判断HMGB1在肝组织中的表达。结果 (1)正常肝、CHB、癌旁组织、肝癌组织中的HMGB1的表达差异有统计学意义(H=7.29,P=0.027);(2)肝组织炎症不同分级与HMGB1的表达有等级相关关系(rs=0.318,P=0.031),肝组织纤维化不同程度与HMGB1的表达有等级相关关系(rs=0.296,P=0.042);(3)肝癌组织中HMGB1的表达与肝癌Edmondson分级间无等级相关关系(rs=0.206,P>0.05)。结论 HMGB1有可能作为反映肝脏炎症和纤维化的间接指标,与肝癌的发生发展密切相关。
- HMGB1蛋白质 /
- 肝肿瘤 /
- 肝炎,乙型,慢性 /
- 基因表达
Abstract: Objective To investigate the expression of high mobility group box 1 protein (HMGB1) in different liver tissue and its relationship with liver tissue injury and hepatocarcinogenesis as well as development.Methods 20 hepatocellular carcinoma and 20 paracancerous tissues specimens were incorporated in this study.Chemicopathological method was used to evaluate the hepatic inflammation degree and fibrosis stage in 46 CHB and 20 para-cancerous tissues;immunohistochemical method was used to detect the expression of HMGB1 in the above liver tissues.Results There was significant difference in the expression of HMGB1 among normal liver, chronic hepatitis B, paracancer tissue and tumor tissue (H=7.29, P=0.027) ;There was a rank correlation between different grade of liver inflammation and the expression level of HMGB1 (rs=0.318, P=0.031) ;There was a rank correlation between varying degrees of liver fibrosis and the expression of HMGB1 (rs=0.296, P= 0.042) ;There was no rank correlation between the HMGB1 expression in hepatocellular carcinoma and Edmondson (rs=0.206, P>0.05) .Conclusion HMGB1 would be an indirect indicators of liver inflammation and fibrosis and closely related to the development of liver cancer.
- HMGBI protein /
- liver neoplasms /
- hepatitis B /
- chronic

HTML全文

参考文献(17)

[1]Wang H, Yang H, Tracey KJ.Extracellular role of HMGB1 in in-flammation and sepsis[J].J Intern Med, 2004, 255 (3) :320-331.

[2]赵雷, 刘靖华, 唐靖, 等.人高迁移率族蛋白1的原核细胞表达及其功能研究[J].中国病理生理杂志, 2006, 22 (6) :1119-1123.

[3]王树彦, 陈宗祐.高迁移率族蛋白1与肿瘤[J].上海医学, 2009, 32 (8) :757-760.

[4] 中华医学会.慢性乙型肝炎防治指南[J].胃肠病学, 2006, 11 (9) :550-556.

[5]中华医学会.病毒性肝炎防冶方案[J].中华肝脏病杂志, 2000, 8 (3) :324-329.

[6]Ishak K, Baptista A, Bianchi L, et al.Histological grading andstaging of chronic hepatitis[J].J Hepatol, 1995, 22 (4) :696-699.

[7]Sappington PL, Yang R, Yang H, et al.HMGB l B box increasesthe permeability of Caco-2 enterocytic monolayers and impairs in-testinal barrier function in mice[J].Gastroenterology, 2002, 123 (4) :790-802.

[8]Sundberg E, Fasth AE, Palmblad K, et al.High mobility group boxchromosomal protein 1 act s as a proliferation signal for activated T lym-phocytes[J].Immunobiology, 2009, 214 (2) :303-309.

[9]Erlandsson Harris H, Andersson U.Mini review:The nuclear pro-tein HMGB l as a proinflammatory mediator[J].Eur J Immunol, 2004, 34 (6) :1503-1512.

[10]赵中夫, 韩德五.高迁移率族蛋白B-1及其在肝损伤合并内毒素血症中的作用[J].中华肝脏病杂志, 2006, 14 (9) :718-720.

[11]刘洪波, 范学工, 黄建军, 等.乙型肝炎患者血清高迁移率蛋白-1含量的检测及临床意义[J].中华肝脏病杂志, 2007, 15 (11) :812-815.

[12]刘洪波, 范学工, 刘悦晖, 等.肝损伤模型小鼠高迁移率族蛋白-1的表达[J].生命科学研究, 2008, 12 (2) :169-173.

[13]Müller S, Scaffidi P, Degryse B, et al.New EMBO members’re-view:the double life of HMGB1 chromatin protein:architecturalfactor and extracellular signal[J].EMBO J, 2001, 20 (16) :4337-4340.

[14]宋舟, 刘屹, 包国强, 等.HMGB1在大肠癌组织中的表达及意义[J].现代肿瘤医学, 2008, 16 (7) :1911-1194.

[15]Campana L, Bosurgi L, Rovere-Querini P.HMGB1:a two-headed signal regulating tumor progression and immunity[J].CurrOpin Immunol, 2008, 20 (3) :518-523.

[16]Cheng BQ, Jia CQ, Liu CT et al.Serum high mobility group boxchromosomal protein 1 is associated with clinicopathologic features inpatients with hepatocellular carcinoma[J].Dig Liver Dis, 2008, 40 (6) :446-452.

[17]Chung Hw, Lee SG, Kim H, et al.Serum high mobility group box-1 (HMGB1) is closely associated with the clinical and pathologic featuresof gastric cancer[J].J Transl Med, 2009, 7 (1) :38.

施引文献

资源附件(0)

访问统计