肝细胞癌中耐药基因的表达及意义

李海平; 陶建蜀; 高波; 余宗涛; 李文翠; 张吉才

肝细胞癌中耐药基因的表达及意义

1. 湖北医药学院附属太和医院
2. 贵阳医学院

详细信息

中图分类号: R735.7
计量
- 文章访问数: 3385
- HTML全文浏览量: 21
- PDF下载量: 646
- 被引次数: 0
出版历程
- 出版日期: 2011-09-20

The expression and clinical significance of resistance gene in hepatocellular carcinoma

摘要

摘要: 目的通过对肝细胞癌（HCC）中3种耐药基因MDR1、MRP、GST-π的检测,探讨肝细胞癌中耐药基因的表达特点及临床意义。方法应用逆转录-聚合酶链反应（RT-PCR）技术检测3种耐药基因在51例肝细胞癌组织和10例正常肝组织中的表达。结果（1）MDR1、MRP、GST-π在肝细胞癌中的表达分别为0.55±0.27、0.62±0.29、0.64±0.31,正常肝组织中的表达分别为0.23±0.10、0.25±0.07、0.26±0.12,耐药基因在肝细胞癌中的表达高于正常肝组织,差异具有统计学意义（P<0.05）;（2）耐药基因的表达与肿瘤Edmondson分级呈正相关（P<0.05）;（3）MRP与GST-π的表达相关。结论肝细胞癌中存在有原发性耐药的现象,且多种机制并存。MDR1、MRP、GST-π在肝细胞癌中有较高的表达。联合检测对制定科学有效的治疗方案有一定价值。
- 癌,肝细胞 /
- 基因表达
Abstract: Objective To detect the expression of three multidrug resistance genes (MDR1、MRP、GST-π) and evaluate clinical significances of multidrug resistance gene in hepatocellular carcinoma (HCC) .Methods RT-PCR was used to examine the expression of 3 resistance genes in 51 HCC cases and 10 normal tissues.Results The expression of MDR1、MRP、GST-π were 0.55±0.27, 0.62±0.29, 0.64±0.31 in hepatocellular cancer tissue and 0.23±0.10, 0.25±0.07, 0.26±0.12 in normal hepatic-tissue (P<0.05) .The expressions of MDR1、MRP、GST-π in HCC were higher in hepatocellular carcinoma tissue than those in normal tissue.The expression of multidrug resistance genes were positively related to tumor Edmondson grade (P<0.05) .The expression of MRP were correlated with the expression of GST-π.Conclusion Primary resistance existed in HCC and there were many mechanisms of multidrug resistance.MDR1, MRP, GST-π in hepatocellular carcinoma had a higher expression.Combined detection of LRP and GST-π expression maybe efficient in clinical treatment.
- carcinoma /
- hepatocellular

HTML全文

参考文献(9)

[1] 陆再英.内科学[M].北京:人民卫生出版社, 2008:461-462.

[2]Warmann S, G hring G, Teichmann B, et al.P-glycopro-tein modulation improves in vitro chemosensitivity in malig-nant pediatric liver tumors[J].Anticancer Res, 2003, 23 (6C) :4607-4611.

[3]Ishikawa Y, Nagai J, Okada Y, et al.Function and expres-sion of ATP-binding cassette transporters in cultured humanY79 retinoblastomacells[J].Biol Pharm Bull, 2010, 33 (3) :504-511.

[4]Shao J, Stapleton PL, Lin YS, et al.Cytochrome p450 andglutathiones-transferase mRNA expression in human fetalliver hematopoietic stem cells[J].Drug Metab Dispos, 2007, 35 (1) :168-175.

[5]Murakami S.Molecular mechanism of multi-drug resistance[J].Nippon Rinsho, 2008, 66 (1) :193-203.

[6]钟向阳, 褚忠华, 曹铭辉, 等.多药耐药基因在原发性肝癌的表达及其临床意义[J].中华实验外科杂志, 2005, 22 (9) :1064-1065.

[7]王伟章, 毛建文, 金小宝, 等.姜黄素对肝癌细胞耐药基因表达的影响[J].实用医学杂志, 2010, 26 (13) :2285-2288.

[8]魏民, 张阳德, 何剪太.姜黄素逆转肝癌耐药性实验研究[J].中国现代医学杂志, 2008, 18 (17) :2441-2443.

[9]高国兰, 涂开家, 刘福军.卵巢癌多药耐药基因的表达及其逆转[J].临床肿瘤学杂志, 2010, 15 (10) :870-873.

施引文献

资源附件(0)

访问统计