左归丸粗提取物对骨髓干细胞转化肝细胞的影响

李瀚旻; 高翔; 晏雪生

左归丸粗提取物对骨髓干细胞转化肝细胞的影响

湖北中医药大学附属医院肝病研究所

基金项目:

国家自然科学基金(90709041,30672590,30271562,30371787,81102531)；

详细信息

中图分类号: R285
计量
- 文章访问数: 3475
- HTML全文浏览量: 16
- PDF下载量: 646
- 被引次数: 0
出版历程
- 出版日期: 2012-03-20

The influence of Zuogui Wan Extract on bone marrow stem cell transforming liver cell

Research funding:

摘要

摘要: 目的观察左归丸及其拆方的粗提取物对骨髓干细胞转化肝细胞的影响,为进一步探索左归丸的有效成分奠定实验基础。方法利用透过性支持物（聚酯TranswellR透明嵌套）建立骨髓干细胞-肝组织细胞共培养体系,采用水煮醇沉方法提取制备左归丸及其拆方粗提取物加入共培养体系。骨髓干细胞与肝细胞共培养7、14、21、28、35d时分别取透过性支持物中的骨髓干细胞爬片用PAS法检测糖原,免疫细胞化学方法（ICC法）检测肝细胞标志物（AFP、CK18、Alb）。结果左归丸及其拆方粗提取物浓度高于1.56 mg/ml时,影响骨髓干细胞的存活,而浓度等于1.56 mg/ml时,共培养条件下骨髓干细胞的糖原、AFP、Alb、CK18的表达量,平行对照各组差异无统计学意义（P>0.05）。结论骨髓干细胞对培养条件比较苛刻,一般的中药粗提制剂不适于骨髓干细胞-肝细胞共培养体系的实验。
- 左归丸 /
- 骨髓祖代细胞 /
- 肝细胞
Abstract: Objective To observe the influence of Zuogui Wan extract on bone marrow stem cell transforming liver cell, and establish experimental basis to further study effective constituent of Zuogui Wan.Methods TranswellR insert was used to establish bone marrow stem cell-liver cell co-culture system.Zuogui Wan extract was prepared by water boiled-alcohol sink method, and was added to co-culture system.Bone marrow stem cell in TranswellR insert was detected after co-culture 7, 14, 21, 28 and 35 days.Glycogen was detected by PAS method, liver cell markers (AFP, Alb and CK18) were detected by immunocytochemistry method.Results Zuogui Wan extract influenced bone marrow stem cell survive when its concentration higher than 1.56mg/ml.The expression of glycogen, AFP, Alb and CK18 in bone marrow stem cell had no significant difference contrasted to control groups, when Zuogui Wan extract's concentration was 1.56mg/ml in co-culture system.Conclusion Bone marrow stem cell was sensitive to culture condition.Traditional Chinese medicine extract prepared by water boiled-alcohol sink method was unfit for experiment of bone marrow stem cell-liver cell co-culture system.
- ZUOGUI PILL /
- myeloid progenitor cells /
- hepatocytes

HTML全文

参考文献(17)

[1]李瀚旻, 高翔, 晏雪生, 等.左归丸促进骨髓形成肝细胞的研究[J].世界华人消化杂志, 2005, 13 (24) :2818-2822.

[2]李瀚旻, 高翔, 晏雪生, 等.左归丸促进骨髓形成肝细胞的分子机制研究[J].中医杂志, 2006, 47 (10) :778-780.

[3]李瀚旻.全面系统深入地研究中医药调控肝再生 (专论) [J].中西医结合肝病杂志, 2007, 17 (3) :129-132.

[4]李瀚旻, 桂文甲, 李晶津, 等.左归丸对同种异性骨髓移植小鼠肝再生相关基因信号通路的影响[J].中国组织工程研究与临床康复, 2008, 12 (31) :6069-6073.

[5]李瀚旻.中医再生医学概论[J].中华中医药学刊, 2008, 26 (11) :2309-2312.

[6]李瀚旻.论“肝主生发”[J].中华中医药学刊, 2009, 27 (10) :2021-2025.

[7]李瀚旻.肾藏精的科学内涵[J].中医杂志.2009, 50 (12) :1061-1064.

[8]高翔, 李瀚旻, 晏雪生.左归丸对同种异性骨髓移植小鼠肝组织Wnt信号通路的影响[J].中西医结合肝病杂志, 2010, 20 (1) :29-31.

[9]李瀚旻.肝藏象肝脏中心说[J].世界中医药杂志, 2011, 6 (1) :11-15.

[10]李瀚旻.虚证本质与生机学说[J].中华中医药学刊, 2011, 29 (10) :2157-2160.

[11]李瀚旻, 晏雪生, 罗建君, 等.左归丸药物血清对骨髓间质细胞转化为肝细胞的作用[J].中国组织工程研究与临床康复, 2007, 11 (28) :5465-5468.

[12]李瀚旻, 高翔, 晏雪生.基于骨髓干细胞与肝组织细胞共培养体系的左归丸血清药理学研究[J].中国组织工程研究与临床康复, 2010, 14 (19) :3527-3532.

[13]Koc ON, Gerson SL, Cooper BW, et al.Rapid hematopoiet-ic recovery after coinfusion of autologous blood stem cells and culture expanded marrow mesenchymal stem cells in ad-vanced breast cancer patients receiving high dose chemo-therapy[J].J Clin Oncol, 2000, 18 (2) :307-316.

[14]Noort WA, Kruisselbrink AB, in't Anker PS, et al.Mesen-chymal stem cells promote engraftment of human umbilical cord blood-derived CD34 (+) cells in NOD/SCID mice[J].Exp Hematol, 2002, 30 (8) :870-878.

[15]Abrahamsson CK, Yang F, Park H, et al.Chondrogenesis and mineralization during in vitro culture of human mesenchy-mal stem cells on three-dimensional woven scaffolds[J].Tissue Eng Part A, 2010, 16 (12) :3709-3718.

[16]Gargett CE, Schwab KE, Zillwood RM, et al.Isolation and culture of epithelial progenitors and mesenchymal stem cells from human endometrium[J].Biol Reprod, 2009, 80 (6) :1136-1145.

[17]Ninomiya Y, Sugahara-Yamashita Y, Nakachi Y, et al.De-velopment of a rapid culture method to induce adipocyte dif-ferentiation of human bone marrow-derived mesenchymal stem cells[J].Biochem Biophys Res Commun, 2010, 394 (2) :303-308.

施引文献

资源附件(0)

访问统计