碱性成纤维细胞生长因子与慢性乙型肝炎及肝纤维化临床检测指标的关系

毛小荣; 岳伟; 朱骏; 张海云; 薛苗

碱性成纤维细胞生长因子与慢性乙型肝炎及肝纤维化临床检测指标的关系

1. 兰州大学第一医院传染科
2. 兰州大学第一医院病理科
3. 兰州窑街煤电公司医院感染科

基金项目:

中央高校基本科研业务费专项资金资助(lzujbky-2009-150)；

详细信息

中图分类号: R575.2
计量
- 文章访问数: 3434
- HTML全文浏览量: 17
- PDF下载量: 672
- 被引次数: 0
出版历程
- 出版日期: 2012-04-20

The study on relationship of basic fibroblast growth factor with the clinical significance on chronic viral hepatitis B and liver fibrosis

Research funding:

摘要

摘要: 目的观察碱性成纤维细胞生长因子（b-FGF）在不同肝纤维化分期（S）肝组织中表达的差异性及其与临床检测常用的肝功能指标、肝纤维化指标、HBV DNA复制水平以及肝硬化Child-Pugh分级之间的关系。方法选取122例HBV引起的慢性肝炎及肝硬化患者,空腹静脉血检测:（1）肝功能指标:ALT、TBil、Alb;（2）肝纤维化指标:透明质酸（HA）、Ⅲ型前胶原（PCⅢ）、层粘连蛋白（LN）、Ⅳ型胶原（CⅣ）;（3）HBV DNA复制水平;（4）对临床诊断肝硬化患者计算其Child-Pugh评分。行肝脏穿刺活检术后,经病理作纤维化分期（S1～S4）。免疫组织化学法测定肝组织中b-FGF在不同病理分期的表达及定位特点,半定量分析法计算b-FGF值,观察b-FGF与肝功能指标、肝纤维化指标、HBV DNA水平以及肝硬化Child-Pugh分级之间的关系。结果（1）肝纤维化S1～S4期肝组织中b-FGF的表达部位及水平不同;（2）肝纤维化S1～S4期肝细胞中b-FGF的表达与肝纤维化分期（S1～S4）呈显著正相关（r=0.542,P<0.01）;（3）不同肝纤维化分期中b-FGF的表达差...
- 成纤维细胞生长因子2 /
- 肝硬化 /
- 肝炎,乙型,慢性
Abstract: Objective To observe expressions of b-FGF in different stage on the hepatic fibrosis and to discuss the relationship with indexes of hepatic function, indexes of hepatic fibrosis, hepatic cirrhosis Child-Pugh classification, HBV DNA replication.Methods 122 cases of HBV-induced chronic hepatitis and cirrhosis were selected.Delect hepatic function (ALT, TBil, Alb) , indexes of liver fibrosis: hyaluronic acid (HA) , Ⅲ procollagen (PC Ⅲ) , laminin (LN) , Ⅳ collagen (CⅣ) , HBV DNA replication were tested and hepatic cirrhosis Child-Pugh classification were judged.the pathological diagnosis and different stages of the hepatic fibrosis of 122 patients with chronic hepatitis were determined by liver biopsy.b-FGF Value was calculated, the relationship of b-FGF with indexes of hepatic function, indexes of hepatic fibrosis, HBV DNA replication, hepatic cirrhosis Child-Pugh classification was observed.Results 1.There were some difference of expressions of b-FGF in different parts and levels of liver fibrosis S1-S4 stage.2.The expressions of b-FGF were gradually increased with the development of the hepatic fibrosis (S1-S4) and positive correlation was determined (r=0.542, P<0.01) .3.In the different processes of the hepatic fibrosis (S1-S4) the expressions of b-FGF with ALT, TBil, ALB, HBV DNA replication were not statistically significant (P>0.05) ;4.In the different processes of the hepatic fibrosis (S1-S4) the expressions of b-FGF with HA, PC Ⅲ, LN, CⅣ were positively correlated.5.The expressions of b-FGF and Child-Pugh classification had no correlation.Conclusion The expressions of b-FGF have a positive correlation with the level of hepatic fibrosis and indexes of hepatic fibrosis and there is no correlation of expressions of b-FGF with ALT, TBil, Alb, Child-Pugh score and HBV-DNA replication.
- fibroblast growth factor 2 /
- liver cirrhosis /
- hepatitis B

HTML全文

参考文献(10)

[1] 陆再英, 钟南山.内科学[M].第7版.北京:人民卫生出版社, 2008:368.

[2]池肇春.慢乙肝抗病毒的个体化治疗[J].临床肝胆病杂志, 2010, 26 (1) :4-8.

[3]康海燕, 董江龙, 王建彬, 等.碱性成纤维细胞生长因子在肝癌及慢乙肝中的表达研究[J].临床肝胆病杂志, 2010, 26 (1) :80-81.

[4]Nugent MA, Iozzo RV.Fibmblast growth flactor-2[J].Int JBiochem&Cell Biology, 2002, 32 (2) :115.

[5]中华医学会肝病学分会, 中华医学会感染学分会.慢性乙型肝炎防治指南[J].临床肝胆病杂志, 2006, 22 (1) :3-15.

[6]李冬, 戴立里, 余斌斌, 等.丹参素对大鼠肝星状细胞增殖、凋亡及NF-κB活性的影响[J].第三军医大学学报, 2009, 31 (8) :724-728.

[7]钟巍, 谢渭芬.肝纤维化治疗新策略[J].临床肝胆病杂志, 2011, 27 (3) :233-235.

[8]Y Li, J Chen, C Wu, et al.Hepatitis B virus/hepatitis C virusupregulate angiopoietin-2 expression through mitogen-ac-tivated protein kinase pathway[J].Hepatol Res, 2010, 40 (10) :1022-1033.

[9]康海燕, 董江龙, 王建彬, 等.肝组织碱性成纤维细胞生长因子与乙肝患者肝纤维化关系的研究[J].临床肝胆病杂志, 2008, 24 (6) :454-455.

[10]Fung J, Lai CL, Seto WK, et al.Nucleoside/nucleotide ana-logues in the treatment of chronic hepatitis B[J].J Antimi-crob Chemother, 2011, 66 (12) :2715-2725.

施引文献

资源附件(0)

访问统计