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Gene copy number variations of BCL2L11 and IL7R in differently progressing patients with chronic HBV infection
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摘要: 目的检测慢性HBV感染不同疾病进展组患者BCL2L11、IL7R基因拷贝数,研究宿主遗传背景差异与疾病进展的关系。方法选择健康对照组20例,慢性HBV携带者组18例,慢性重型肝炎组32例和原发性肝癌组18例。提取外周血基因组DNA并利用Tanqman real-time PCR方法测定BCL2L11、IL7R的拷贝数。结果四组之间BCL2L11的基因拷贝数差异有统计学意义（P<0.05）,而四组之间IL7R的基因拷贝数差异无统计学意义（P>0.05）。肝癌组BCL2L11拷贝数明显低于另三组（P<0.05）;重肝组、携带组与对照组间差异无统计学意义（P>0.05）。结论不同进展程度肝损害组间BCL2L11存在基因拷贝数变异（CNVs）,但这些变异不是影响其基因表达的主要因素,因此不是影响慢性HBV感染过程中肝脏炎症程度的主要因素。Abstract: Objective To detect the copy numbers of the BCL2-like 11 apoptosis facilitator (BCL2L11) gene and the cytokine interleukin 7 receptor (IL7R) gene in patients with chronic hepatitis B virus (HBV) infection, and determine the relation between copy number variants (CNVs) and differential disease progression.Methods Study participants were recruited from the hospital setting from June 2006 to December 2008, and included four groups composed of uninfected healthy controls (n=20) , chronic HBV carriers (n=18) , chronic severe hepatitis patients (n=32) , and patients with HBV-associated hepatocellular carcinoma (HCC;n=18) .Patient DNA was extracted from peripheral blood and applied as template to TaqMan real-time PCR to measure gene copy numbers of BCL2L11 and IL7R.Results The BCL2L11 gene copy number was significantly different among the four groups (P<0.05) .In contrast, the IL7R gene copy number was not significantly different among the four groups P>0.05) .The BCL2L11 gene copy number was significantly lower in the HCC group compared to the other three groups (P<0.05) , while the chronic severe hepatitis group, HBV carrier group, and control group showed statistically similar gene copy numbers (P>0.05) .Conclusion CNVs of BCL2L11 exist in HBV-infected patients with different levels of liver damage.Future studies should address the contributions of these CNVs to the degree of liver inflammation during chronic HBV infection.
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Key words:
- gene copy number variation /
- hepatitis B /
- chronic
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