ω-3多不饱和脂肪酸在体外胰腺腺泡细胞培养体系中的炎症调控作用及机制

程锐; 蔡欣然; 周浩辉; 韩圣华; 朱金海; 陈辉星; 陈燕凌

ω-3多不饱和脂肪酸在体外胰腺腺泡细胞培养体系中的炎症调控作用及机制

福建医科大学附属协和医院肝胆外科

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中图分类号: R657.51
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- 出版日期: 2012-08-20

Omega-3 polyunsaturated fatty acids inhibit inflammation during the early stage of acute pancreatitis

摘要

摘要: 目的探讨ω-3多不饱和脂肪酸（ω-3 PUFAs）对急性胰腺炎早期炎症反应的作用。方法培养胰腺腺泡细胞系AR42J,随机分为A组（空白对照）、B组（ω-3 PUFAs预处理后蛙皮素刺激）、C组（仅用蛙皮素刺激）;实时荧光定量PCR检测各组细胞肿瘤坏死因子（TNF）α、白细胞介素（IL）-6和线粒体解偶联蛋白-2（UCP-2）mRNA表达;Western Blot检测细胞核中NF-κB p65亚单位的含量;Hoechst33342/PI染色检测细胞坏死。结果 B组TNFα、IL-6、UCP-2 mRNA表达水平、细胞核NF-κBp 65含量及细胞坏死率与C组相比均明显减少,差异有统计学意义[（0.69±0.10）vs（1.34±0.19）,P<0.001;（0.69±0.06）vs（1.39±0.06）,P<0.001;（0.58±0.12）vs（1.26±0.07）,P<0.001;（0.54±0.09）vs（0.92±0.09）,P<0.001;（0.20±0.01）vs（0.35±0.03）,P<0.001;F=62.718～157.426,P均<...
- 胰腺炎,急性 /
- ω-3多不饱和脂肪酸 /
- 炎症
Abstract: Objective To investigate the therapeutic effects of ω-3 polyunsaturated fatty acids (PUFAs) during the early stage of acute pancreatitis. Methods AR42J pancreatic acinar cells were divided into three groups: A, untreated control;B, pretreated with the ω-3 PUFA docosahexaenoic acid (DHA) and stimulated with cerulein to simulate the inflammatory response of acute pancreatitis;and C, stimulated with cerulein only.Expression of inflammatory cytokines (TNFα and IL-6) and a regulator of reactive oxygen species (UCP2) was measured by real-time PCR.Expression of the NF-κB p65 subunit in nuclear protein was detected by Western blotting.Necrotic and apoptotic cells were detected by staining with Hoechest33342 and propidium iodide, respectively. Results Cerulein-stimulated cells had significantly higher levels of TNFα, IL-6, and UCP-2 mRNA expression, NF-κB p65 protein, and cell necrosis (groups B and C vs group A, P<0.05) .DHA pretreatment significantly reduced the levels of all cerulein-stimulated changes (group B vs group C, P<0.05) . Conclusion ω-3 PUFAs may inhibit the inflammatory reaction during the early stage of acute pancreatitis.
- pancreatitis /
- acute /
- ω-3 polyunsaturated fatty acids

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