唐山地区献血人群隐匿性乙型肝炎病毒感染分析

曹晓; 曹庆宝; 张国强; 常在娟; 李淑萍; 徐微微

唐山地区献血人群隐匿性乙型肝炎病毒感染分析

1. 唐山市中心血站
2. 唐山市第三医院

详细信息

中图分类号: R512.62
计量
- 文章访问数: 2879
- HTML全文浏览量: 17
- PDF下载量: 715
- 被引次数: 0
出版历程
- 出版日期: 2013-02-20

Analysis of occult hepatitis B virus infection among blood donors in Tangshan, China

摘要

摘要:
目的了解唐山地区无偿献血人群隐匿性乙型肝炎感染情况。方法用ELISA法检测无偿献血者的乙型肝炎血清标志物,对于HBsAg阴性样本,进行HBV核酸检测（NAT）,NAT阳性样本,用罗氏试剂确证HBV DNA载量。结果共检测116 741例血样,证实隐匿性乙型肝炎感染者35例,占总献血人数的0.29‰。其中97.1%隐匿性乙型肝炎感染者样本的HBV DNA滴度低于102IU/ml。在HBV DNA阳性人群中,抗-HBc阳性率较高,占81.5%,抗-HBs阳性或乙型肝炎病毒血清标志物全阴性也可检出HBV DNA分别占55.6%和22.9%。结论唐山地区献血人群中血清HBsAg阴性者存在一定比例的隐匿性HBV感染,其HBV病毒载量均较低,核酸检测能够提高HBV感染的检出率。
- 肝炎,乙型 /
- 肝炎病毒,乙型 /
- 酶联免疫吸附测定
Abstract:
Objective To investigate the prevalence of occult hepatitis B virus (HBV) infection (OBI) among the volunteer blood donors in Tangshan, China.Methods Enzyme-linked immunosorbent assay was used to detect serum HBV markers in the blood donors.Nucleic acid test (NAT) was performed in the hepatitis B surface antigen (HBsAg) -negative blood samples to detect HBV DNA, and Roche reagent was used to measure the viral load of HBV DNA in NAT-positive samples.Results Among the HBsAg-negative blood samples of 116 741 blood donors, 39 (0.033%) had positive results in NAT, and 35 (0.029%) were confirmed with OBI.The viral load of HBV DNA was less than 102 IU/ml in 97.1% of the blood donors with OBI.Of the blood donors with OBI, 27 (77.1%) were positive for at least one of hepatitis B surface antibody (HBsAb) , hepatitis B e antigen, hepatitis B e antibody, and hepatitis B core antibody (HBcAb) , and 8 (22.9%) were negative for all serum HBV markers.Of the 27 positive blood donors, 22 (81.5%) were positive for HBcAb, and 15 (55.6%) were positive for HBsAb.Conclusion OBI occurs in some HBsAg-negative blood donors in Tangshan, and the viral load of HBV DNA is low.NAT is effective in increasing the detection rate of HBV infection.
- hepatitis B /
- hepatitis B virus /
- enzyme-linked immunosorbent assay

HTML全文

参考文献(12)

[1]Squadrito G, Orlando ME, Pollicino T, et al.Virological pro-files in patients with chronic hepatitis C and overt or occultHBV infection[J].Am J Gastroenterol, 2002, 97 (6) :1518-1523.

[2]Feagins AR, Opriessnig T, Guenette DK, et al.Detectionand characterization of infectious hepatitis E virus from com-mercial pig livers sold in local grocery stores in the USA[J].J Gen virol, 2007, 88 (Pt 3) :912-917.

[3]Saraswat S, Banerjee K, Chaudhury N, et al.Post-transfu-sion hepatitis type B following multiple transfusions of HBsAg-negative blood[J].J Hepatol, 1996, 25 (5) :639-643.

[4]Satake M, Taira R, Yugi H, et al.Infectivity of blood compo-nents with low hepatitis B virus DNA levels identified in alookback program[J].Transfusion, 2007, 47 (7) :1197-1205.

[5]Wang LH, Xie XL, Shang GF, et al.NAT testing for HBV, HCV, HIV on donors'samples negative by ELISA tests[J].Chin J Blood Transfusion, 2005, 18 (4) :286-289. (in Chi-nese) 王良华, 叶贤林, 尚桂芳, 等.免疫筛查阴性献血者血样病毒核酸检测的研究[J].中国输血杂志, 2005, 18 (4) :286-289.

[6]Wang QM, Jiang NZ, Xiao JY, et al.Investigation of occulthepatitis B virus infection among blood donors in Jiangsu area[J].J Clin Transfus Lab Med, 2012, 14 (2) :119-120. (inChinese) 王庆敏, 蒋昵真, 肖建宇, 等.江苏地区献血者隐匿性HBV感染情况调查[J].临床输血与检验, 2012, 14 (2) :119-120.

[7]Chen CR, Yuan Q, Ge SX, et al.Surveillance for occult HBVinfection and HBsAg variants in blood donors[J].Chin J Vir-ol, 2009, 25 (3) :178-184. (in Chinese) 陈长荣, 袁权, 葛胜祥, 等.无偿献血者中隐匿性乙型肝炎病毒感染及表面抗原突变分析[J].病毒学报, 2009, 25 (3) :178-184.

[8]江红, 汤正好, 臧国庆.隐匿性乙型肝炎[J].临床肝胆病杂志, 2004, 20 (5) :311-312.

[9]Chevrier MC, St-Louis M, Perreault J, et al.Detection andcharacterization of hepatitis B virus of anti-hepatitis B coreantigen-reactive blood donors in Quebec with an in-housenucleic acid testing assay[J].Transfusion, 2007, 47 (10) :1794-1802.

[10]Minuk GY, Sun DF, Uhanova J, et al.Occult hepatitis B vi-rus infection in a North American community-based popula-tion[J].J hepatol, 2005, 42 (4) :480-485.

[11]Torbenson M, Thomas DL.Occult hepatitis B[J].Lancetinfect dis, 2002, 2 (8) :479-486.

[12]Mulrooney-Cousins PM, Michalak TI.Persistent occult hepati-tis B virus infection:experimental findings and clinical implica-tions[J].World J Gastroenterol, 2007, 13 (43) :5682-5686.

施引文献

资源附件(0)

访问统计