生物型人工肝支持系统种子细胞的来源与应用

朱雪晶; 黄伟健; 鄢和新

doi:10.12449/JCH240205

生物型人工肝支持系统种子细胞的来源与应用

DOI: 10.12449/JCH240205

朱雪晶¹,
黄伟健^{1, 2},
鄢和新^{1, 2, , ,}

1.
上海赛立维生物科技有限公司，上海 201210
2.
上海交通大学医学院附属仁济医院麻醉科，上海 200127

基金项目:

国家自然科学基金 (82270635);

上海市2020年度“科技创新行动计划”生物医药科技支撑专项 (20S31906600)

利益冲突声明：本文不存在任何利益冲突。

作者贡献声明：朱雪晶、黄伟健负责文献资料搜集和分析，撰写论文；鄢和新负责拟定写作思路，指导撰写文章并最后定稿。

详细信息

通信作者:
鄢和新， hexinyw@163.com （ORCID： 0000-0001-8390-650X）

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出版历程
- 收稿日期: 2023-10-25
- 录用日期: 2023-11-27
- 出版日期: 2024-02-19

Source and application of seed cells in bioartificial liver support system

Xuejing ZHU¹,
Weijian HUANG^{1, 2},
Hexin YAN^{1, 2
, ,
,}

1.
Shanghai Celliver Biotechnology Co.，Ltd.，Shanghai 201210，China
2.
Department of Anesthesiology and Critical Care Medicine，Renji Hospital，Shanghai Jiao Tong University School of Medicine，Shanghai 200127，China

Research funding:

National Natural Science Foundation of China (82270635);

Shanghai 2020“Science and Technology Innovation Action Plan” Biomedical Science and Technology Support Project (20S31906600)

More Information

Corresponding author: YAN Hexin， hexinyw@163.com （ORCID： 0000-0001-8390-650X）

摘要

摘要: 迄今为止，重症肝炎、肝衰竭尚无特效治疗方法，病死率高达70%，是国内外危重症中的治疗难点。肝移植术是目前终末期肝病最有效的治疗方法，然而仅有1%～2%的患者能够获得器官移植机会。生物型人工肝支持系统通过体外机械、理化以及生物装置，清除患者体内蓄积的各种有害物质，代偿肝脏代谢功能，补充必需物质，改善内环境，帮助患者恢复肝功能，度过危险期，亦为患者肝移植争取宝贵时间，因此被认为是治疗终末期肝病的重要方法之一。生物型人工肝的核心要素是肝细胞，本综述总结了当前生物型人工肝主要的肝种子细胞来源、3D培养方法以及相应的生物反应器培养系统，期望逐步实现肝细胞体外规模化制备，从而获得足够数量和质量的肝细胞这一临床应用亟待解决的核心问题。
- 肝，人工 /
- 种子细胞 /
- 培养技术
Abstract: So far， there are still no specific treatment methods for severe hepatitis and liver failure， resulting in a mortality rate of over 70%， and they are the difficulties in the treatment of critical illness in China and globally. Liver transplantation is currently the most effective treatment method for end-stage liver disease， but only 1%‍ ‍—‍ ‍2% of patients can receive the opportunity for organ transplantation. The bioartificial liver support system utilizes external mechanical， physical， and biological devices to remove various harmful substances accumulated in the patient’s body， compensate for the metabolic functions of the liver， supplement necessary substances， improve internal environment， promote the recovery of liver function， help patients get through the critical period， and save time for liver transplantation， and therefore， it is considered one of the important methods for the treatment of end-stage liver disease. Since hepatocytes are the core element of bioartificial liver， this article summarizes the sources of liver seed cells， 3D culture methods， and corresponding bioreactor culture systems and hopes to gradually solve the core issue of large-scale in vitro preparation of hepatocytes to obtain hepatocytes with adequate quantity and quality， which urgently needs to be addressed in clinical application.
- Liver， Artificial /
- Cell Sources /
- Culture Techniques

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图 1 生物型人工肝种子细胞来源

Figure 1. Cell sources for bioartificial livers

下载: 全尺寸图片幻灯片

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