基于外周血T淋巴细胞比例特点的慢加急性肝衰竭正虚机制

曹慧; 张旸; 谭善忠; 赵磊; 沈建军; 肖倩; 梁重峰; 蒋淑莲

doi:10.3969/j.issn.1001-5256.2014.04.009

基于外周血T淋巴细胞比例特点的慢加急性肝衰竭正虚机制

DOI: 10.3969/j.issn.1001-5256.2014.04.009

1. 南京市第二医院中西医结合科
2. 南京中医药大学临床医学院

基金项目:

南京市医学科技发展项目(DYY056,ZKX13018)；南京市科技计划项目(201104004)；江苏省“六大人才高峰”项目(苏人社发〔2010〕555号)；江苏省中医药领军人才项目(LJ200922)；江苏省“333高层次人才培养工程”项目(苏人才〔2011〕15号)；

详细信息

中图分类号: R512.62;R575.3
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出版历程
- 收稿日期: 2013-07-29
- 出版日期: 2014-04-20

Relationship between percentages of T lymphocytes in peripheral blood and deficiency of vital qi in patients with HBV- ACLF

Department of Integrated , TCM & Western Medicine, The Second Hospital of Nanjing

Research funding:

摘要

摘要:
目的探讨外周血T淋巴细胞比例特点与HBV相关慢加急性肝衰竭正虚机制的关系。方法选取2010年1月-2012年12月在本院就诊的HBV相关慢加急性肝衰竭早期患者（肝衰竭组）40例与慢性乙型肝炎（CHB）患者（CHB组）50例,以门诊同期健康体检者20例为健康对照组,应用流式细胞仪测定外周血CD3+T淋巴细胞、CD4+和CD8+T淋巴细胞比值、CD4+CD25+调节性T细胞所占比例,比较组间差异。3组间比较采用方差分析。结果肝衰竭组外周血CD3+T淋巴细胞（34.12%±22.33%）、CD4+/CD8+比值（1.78±1.25）和CD4+CD25+调节性T细胞（0.83%±1.00%）所占比例与健康对照组（分别为50.31%±12.09%、1.32%±0.31%、2.93%±1.31%）和CHB组（分别为49.72%±20.11%、1.31%±0.52%、3.17%±2.29%）相比较均下降,差异有统计学意义（P<0.05）。结论 HBV相关慢加急性肝衰竭一旦形成,外周血T淋巴细胞及相关效应T淋巴细胞便处于"耗损"状态,体现其"正虚"的病机特点。
- 肝功能衰竭 /
- 肝炎,乙型,慢性 /
- T淋巴细胞 /
- 中西医结合疗法
Abstract:
Objective To investigate the relationship between percentages of T lymphocytes in peripheral blood and deficiency of vital qi in patients with hepatitis B virus- related acute- on- chronic liver failure (HBV- ACLF) .Methods Forty patients with early- stage HBV-ACLF were enrolled as liver failure group, and 50 patients with chronic hepatitis B (CHB) as CHB group;20 healthy subjects who underwent physical examination in the clinic during the same period were selected as control group.Flow cytometry was used to determine the percentages of CD3+T lymphocytes, CD4+and CD8+T lymphocytes, and CD4+CD25+regulatory T cells (Tregs) in peripheral blood, as well as CD4+/ CD8+ratio, and the three groups were compared in this regard by analysis of variance.Results Compared with the control group and CHB group, the liver failure group had a significantly decreased percentage of CD3+T lymphocytes in peripheral blood (34.12% ± 22.33% vs 50.31% ± 12.09% and 49.72% ± 20.11%, P < 0.05) , a significantly increased CD4+/ CD8+ratio (1.78 ± 1.25 vs 1.32 ± 0.31 and 1.31 ± 0.52, P < 0.05) , and a significantly decreased percentage of CD4+CD25+Tregs in peripheral blood (0.83% ± 1.00% vs 2.93% ± 1.31% and 3.17% ± 2.29%, P < 0.05) .Conclusion Once HBV- ACLF develops, T lymphocyte cells in peripheral blood and related effector T lymphocytes are“exhausted”, which reveals that the characteristics of its pathogenesis is“deficiency of vital qi”.
- liver failure /
- hepatitis B, chronic /
- T lymphocyte /
- TCM WM THERAPY

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