Establishment and management of nonalcoholic fatty liver disease biobank
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摘要:
目的探讨非酒精性脂肪性肝病(NAFLD)血液标本的采集和保存及生物样本库的建立和信息化管理方法。方法收集2009年10月-2013年10月经B超及临床血液学检测诊断为NAFLD的1226例患者的全血标本,进行生化指标测定、血浆及全血基因组DNA提取,并检测DNA的浓度和纯度,分别放至-80℃超低温冰箱储存备用,同时建立一套信息管理系统用于NAFLD生物样本库的管理。结果 1226例NAFLD患者的全血标本,其中包括双生子标本83例及家系标本100例。血浆及DNA的提取成功率均为100%,并随机抽取100份DNA进行检测,结果表明所收集标本能够满足后续试验要求。结论该研究成功建立了NAFLD生物样本库,拥有标准化的信息管理系统,对标本进行质量控制和信息化管理,为进一步研究NAFLD奠定了良好的基础。
Abstract:Objective To investigate the collection and preservation of blood specimens from patients with nonalcoholic fatty liver disease ( NAFLD) and the establishment and information management of biobank. Methods Whole blood samples were collected from 1226 patients who were diagnosed with NAFLD based on B- mode ultrasound and blood tests from October 2009 to October 2013. Biochemical parameters were measured. Plasma and whole- blood genomic DNA was extracted from the samples, and the purity and concentration of DNA were determined. Specimens were preserved in a refrigerator (- 80℃) . An information management system for NAFLD biobank was established. Results Specimens of 1226 NAFLD patients, including those of 83 twins and 100 families, were collected. The success rate was100% for extraction of plasma and whole- blood genomic DNA. One hundred DNA samples were randomly selected for testing, and the results showed that the collected specimens met the requirements of following experiments. Conclusion The NAFLD Biobank has been successfully established in this study. It has the standard information management system and enables the quality control and information management of specimens, laying a solid foundation for further research on NAFLD.
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[1] BHALA N, JOUNESS RI, BUGIANESI E.Epidemiology and natural history of patients with NAFLD[J].Curr Pharm Des, 2013, 19 (29) :5169-5176. [2] WAH-KHEONG C, KHEAN-LEE G.Epidemiology of a fast emerging disease in the Asia-Pacific region:non-alcoholic fatty liver disease[J].Hepatol Int, 2013, 7 (1) :65-71. [3]HOU XH, ZHU YX, LU HJ, et al.Non-alcoholic fatty liver disease's prevalence and impact on alanine aminotransferase associated with metabolic syndrome in the Chinese[J].J Gastroenterol Hepatol, 2011, 26 (4) :722-730. [4]Fatty Liver and Alcoholic Liver Disease Study Group of the Chinese Liver Disease Association.Guidelines for management of nonalcoholic fatty liver disease[J].Chin J Hepatol, 2006, 14 (3) :161-163. (in Chinese) 中华医学会肝脏病学分会脂肪肝和酒精性肝病学组.非酒精性脂肪性肝病诊疗指南[J].中华肝脏病杂志, 2006, 14 (3) :161-163. [5]The Chinese National Workshop on Fatty Liver and Alcoholic Liver Disease for the Chinese Liver Disease Association.Guidelines for management of nonalcoholic fatty liver disease[J].J Clin Hepatol, 2010, 26 (2) :120-124. (in Chinese) 中华医学会肝脏病学分会脂肪肝和酒精性肝病学组.非酒精性脂肪性肝病诊疗指南[J].临床肝胆病杂志, 2010, 26 (2) :120-124. [6]SHI DD, MI YQ.TCM etiology and pathogenesis research on nonalcoholic fatty liver[J].Jilin J Tradit Chin Med, 2012, 32 (6) :545-546. (in Chinese) 史丹丹, 宓余强.非酒精性脂肪肝中医病因病机研究[J].吉林中医药, 2012, 32 (6) :545-546. [7]ROMEO S, KOZLITINA J, XING C, et al.Genetic variation in PNPLA3 confers susceptibility to nonalcoholic fatty liver disease[J].Nat Genet, 2008, 40 (12) :1461-1465. [8] ROTMAN Y, KOH C, ZMUDA JM, et al.The association of genetic variability in patatin-like phospholipase domain-containing protein 3 (PNPLA3) with histological severity of nonalcoholic fatty liver disease[J].Hepatology, 2010, 52 (3) :894-903. [9] KRAWCZYK M, GRNHAGE F, ZIMMER V, et al.Variant adiponutrin (PNPLA3) represents a common fibrosis risk gene:noninvasive elastography-based study in chronic liver diseases[J].J Hepatol, 2011, 55 (2) :299-306. [10] XU J, XIN YN, LYU WH, et al.Polymorphism rs738409 in PNPLA3 is associated with inherited susceptibility to non-alcoholic fatty liver disease[J].Chin J Hepatol, 2013, 21 (8) :619-623. (in Chinese) 徐静, 辛永宁, 吕维红, 等.PNPLA3 rs738409基因多态性与非酒精性脂肪性肝病遗传易感性的关系[J].中华肝脏病杂志, 2013, 21 (8) :619-623. [11]LIN ZH.Genetic pedigree investigation of nonalcoholic fatty liver disease and noninvasive diagnosis of hepatic fibrosis[D].Shandong:Qingdao University, 2011. (in Chinese) 林中华.非酒精性脂肪性肝病遗传学家系研究及肝纤维化无创诊断[D].山东:青岛大学, 2011.引证本文:CHEN LZ, ZHANG HY, JIANG M, et al.Establishment and management of nonalcoholic fatty liver disease biobank[J].J Clin Hepatol, 2014, 30 (9) :906-909. (in Chinese) 陈立震, 张海英, 姜曼, 等.非酒精性脂肪性肝病生物样本库的建立与管理[J].临床肝胆病杂志, 2014, 30 (9) :906-909.
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