Effect of kidney-tonifying and spleen-strengthening prescription on PD-1 and PD-L1 expression in HBeAg-positive chronic hepatitis B patients with slightly increased ALT levels
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摘要:
目的研究补肾健脾方对ALT轻度异常的HBe Ag阳性慢性乙型肝炎(CHB)患者程序性死亡受体(PD)1/程序性死亡配体(PD-L)1表达的影响。方法选取2010年9月1日-2013年12月31日在上海中医药大学附属曙光医院就诊的32例ALT轻度升高的HBe Ag阳性CHB患者。在明确临床疗效的基础上,分离外周血单个核细胞,流式细胞仪检测补肾健脾方治疗前后患者外周血T淋巴细胞上PD-1和树突状细胞(DC)上PD-L1的表达变化。计量资料组间比较采用独立样本t检验,计数资料组间比较采用χ2检验。结果与治疗前相比,治疗组患者外周血CD4+T细胞表达PD-1百分率明显降低(P<0.01),CD4+T细胞表达PD-1的平均荧光强度(MFI)显著下降(P<0.05);治疗后两组患者CD4+T细胞表达PD-1百分率和MFI比较,差异均有统计学意义(P值均<0.05)。治疗组CD8+T细胞表达PD-1百分率和MFI与治疗前相比均明显降低(P值均<0.01);治疗后两组患者CD8+T细胞表达PD-1的MFI比较差异有统计学意义(P<0.05)。与治疗前相比,治疗组DC表达P...
Abstract:Objective To study the effect of kidney-tonifying and spleen-strengthening prescription(KTSSP) on the expression of programmed death-1(PD-1) and programmed death-ligand 1(PD-L1) in HBe Ag-positive chronic hepatitis B(CHB) patients with slightly increased alanine aminotransferase(ALT) levels.Methods This study included 32 HBe Ag-positive CHB patients with slightly increased ALT levels who were admitted to Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine from September1,2010 to December 31,2013.These cases were divided into treatment group and control group using the randomized controlled method.Peripheral blood mononuclear cells were separated,and the expression levels of PD-1 on peripheral blood T lymphocytes and PD-L1 on dendritic cells were measured before and after treatment by flow cytometry.Comparison of continuous data between groups was made by independent-samples t test,while comparison of categorical data between groups was made by chi-square test.Results After treatment,the treatment group had a significantly reduced percentage of peripheral blood CD4+T cells with PD-1 expression(P < 0.01) and a significantly reduced mean fluorescence intensity(MFI) of PD-1 expression on CD4 + T cells(P < 0.05); there were significant differences in the percentage of CD 4+T cells with PD-1 expression and the MFI of PD-1 expression between the treatment group and control group(both P<0.05).After treatment,the treatment group had a significantly reduced percentage of peripheral blood CD8+T cells with PD-1 expression(P < 0.01) and a significantly reduced MFI of PD-1 expression on CD8+T cells(P < 0.01); there were significant differences in the percentage of CD8+T cells with PD-1 expression and the MFI of PD-1 expression between the two groups(both P < 0.05).After treatment,the treatment group had a significantly reduced MFI of PD-L1 expression on dendritic cells(P < 0.05) and myeloid dendritic cells(P < 0.01); there were no significant differences in the expression levels of PD-L1 on dendritic cells and myeloid dendritic cells between the two groups(both P >0.05).Conclusion For HBe Ag-positive CHB patients,KTSSP can reduce the expression levels of PD-1 on peripheral blood CD4+T and CD8+T cells and PD-L1 on dendritic cells and myeloid dendritic cells,which is associated with its clinical efficacy.
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Key words:
- hepatitis B,chronic /
- drug therapy(TCD) /
- strengthening spleen /
- reinforcing kidney
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