Diagnostic value of combined determination of DNA ploidy level and miRNA-21 expression in ascites cells for hepatocellular carcinoma
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摘要:
目的评估联合检测腹腔积液细胞DNA倍体与微小RNA-21(miRNA-21)表达诊断肝细胞癌(HCC)的效能。方法将2012年1月1日-2014年1月1日鄂州市中心医院所收治临床表现伴有腹腔积液的患者纳入研究,HCC患者分入试验组,非HCC患者分入对照组。比较两组患者相关危险因素指标的差异性,采用Logistic回归分析进一步提取独立敏感指标,绘制相应指标的ROC曲线,计算曲线下面积(AUC)及约登指数(YI)。结果两组患者组间资料单因素比较显示:性别(男性比例)、吸烟史、HBV阳性、上腹部CT疑似阳性、卡氏功能量表(KPS量表)、ALT、甲胎蛋白(AFP)、异常凝血酶原(APT)、α-L-岩藻糖苷酶(AFU)、腹水细胞miRNA-21、腹水细胞DNA异倍体阳性共计11项指标差异存在统计学意义(P值均<0.05)。Logistic回归分析显示,吸烟史、AFP、APT、腹水细胞miRNA-21、腹水细胞DNA异倍体是诊断HCC的敏感指标。腹水细胞miRNA-21与DNA异倍体的AUC分别为0.742与0.801;吸烟史、AFP与APT三者联合断HCC的AUC为0.852,YI为0....
Abstract:Objective To evaluate the diagnostic value of combined determination of DNA ploidy level and microRNA-21(miRNA-21)expression in ascites cells for hepatocellular carcinoma(HCC).Methods A total of 117 patients with ascites admitted to our hospital from January 1,2012 to January 1,2014 were enrolled.The patients with a confirmed diagnosis of HCC were assigned to experimental group(n= 48),and those without HCC to control group(n = 69).The risk factors for HCC were compared between the two groups.Logistic regression analysis was used to extract independent sensitive indicators.The receiver operating characteristic(ROC) curve for each indicator was drawn,and the area under the ROC curve(AUC) and Youden index(YI) were calculated.Results Univariate analysis revealed significant differences between the two groups in sex(male ratio),smoking history,positive rate of HBV,proportion of suspected positive cases based on upper abdominal CT,Karnofsky Performance Scale score,alanine aminotransferase,alpha-fetoprotein(AFP),abnormal prothrombin(APT),α-L-fucosidase,miRNA-21 expression in ascites cells,and the positive rate of DNA aneuploidy in ascites cells(all P < 0.05).Logistic regression analysis showed that smoking history,AFP,APT,miRNA-21 expression in ascites cells,and the positive rate of DNA aneuploidy in ascites cells were sensitive indicators for the diagnosis of HCC.The AUCs of miRNA-21 expression and positive rate of DNA aneuploidy were 0.742 and 0.801,respectively; the AUC and YI of combined use of smoking history,AFP,and APT were0.852 and 0.5992,respectively,while the AUC and YI increased to 0.869 and 0.6222,respectively,if including miRNA-21 expression and positive rate of DNA aneuploidy in combined determination.Conclusion DNA aneuploidy and miRNA-21 in ascites cells are independent risk factors for HCC.The two indicators have good diagnostic values when used separately,and they show increased diagnostic values when used in combination with other parameters(smoking history,AFP,and APT).Combined determination of these indicators has great clinical significance for the early diagnosis of HCC and is a good complement to the current HCC diagnosis system.
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Key words:
- carcinoma,hepatocellular /
- ascites /
- DNA /
- microRNAs /
- diagnosis
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