Significance of combined analysis of autoantibodies in diagnosis of primary biliary cirrhosis
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摘要:
目的探讨不同抗体联合检测在原发性胆汁性肝硬化(PBC)诊断中的意义。方法随机选择灵台县妇幼保健站2010年7月-2013年7月收治的肝病患者245例,根据患者所患疾病种类不同,将患者分为PBC组(n=162)、自身免疫性肝炎(AIH)组(n=42)和其他肝病患者(LDC)组(n=41)。采用ELISA法检测血清抗线粒体抗体M2亚型(AMA-M2)、抗SP100以及GP210抗体,采用Western Blot检测法检测血清中抗可溶性肝抗原(抗SLA)抗体,采用间接免疫荧光法检测血清抗线粒体抗体(抗AMA)。组间抗体阳性率的比较采用χ2检验和Fisher’s检验。结果 PBC患者的AMA、AMA-M2、抗GP210的阳性率分别是93.21%、87.65%、19.75%,高于AIH患者的19.05%、7.14%、9.52%,差异具有统计学意义(χ2=97.311,P=0.001;χ2=98.264,P=0.001;χ2=10.312,P=0.012)。PBC患者的AMA、AMA-M2及抗GP210的阳性率分别为93.21%、87.65%、19.75%,LDC患者的阳性率分别为9.76%、2....
Abstract:Objective To evaluate the significance of combined analysis of different autoantibodies in the diagnosis of primary biliary cirrhosis( PBC). Methods Two- hundred and forty- five patients with liver diseases were randomly selected. According to the disease type,patients were divided into PBC group( n = 162),autoimmune hepatitis( AIH) group( n = 42),and liver disease control( LDC) group( n = 41). ELISA assay was used to detect serum anti- mitochondrial antibody( AMA- M2) and antibodies against SP100 and GP210.Western blot assay was employed to detect serum anti- SLA antibodies. Indirect immunofluorescence assay was performed to detect serum AMA. Comparison of antibody positive rates between groups was carried out using the chi- square test and Fisher' s test. Results Serum AMA,AMA- M2,and anti- GP210 positive rates of the PBC group were 93. 21%,87. 65%,and 19. 75%,respectively,significantly higher than those of the AIH group,i. e.,19. 05%,7. 14%,and 9. 52%,respectively( χ2= 97. 311,P = 0. 001; χ2= 98. 264,P =0. 001; χ2= 10. 312,P = 0. 012). The AMA,AMA- M2,and anti- GP210 positive rates of the PBC group were also significantly higher than those of the LDC group,i. e.,9. 76%,2. 44%,and 7. 32%,respectively( χ2= 142. 745,P = 0. 003; χ2= 112. 574,P = 0. 002; χ2= 15. 217,P = 0. 042). Conclusion Serum AMA has a remarkable meaning to PBC diagnosis,anti- SP100 gives a hint of PBC progression,and anti- GP210 plays a role in the diagnosis of AMA- negative PBC.
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Key words:
- liver cirrhosis,biliary /
- hepatitis,autoimmune /
- autoantibodies
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