慢性乙型肝炎患者外周血中T淋巴细胞亚群和CD4+CD25+调节性T淋巴细胞的表达水平与HBV DNA定量的相关分析
DOI: 10.3969/j.issn.1001-5256.2015.04.016
Correlation analysis of percentages of T lymphocyte subsets and CD4~+CD25~+ regulatory T cells and HBV DNA level in peripheral blood of chronic hepatitis B patients
-
摘要: 目的探讨慢性乙型肝炎(CHB)患者外周血中T淋巴细胞亚群(CD3+T、CD4+T、CD8+T、CD4+/CD8+T)和CD4+CD25+调节性T淋巴细胞(Treg)表达水平与HBV DNA定量的相关性。方法选取2012年7月-12月在兰州市第二人民医院就诊的175例HBV感染者作为研究对象,分为CHB组(63例)、HBV携带者(ASC组)(112例),以同期50例健康体检者作为对照组。采用流式细胞术检测3组患者血清中的CD3+T、CD4+T、CD8+T、CD4+T/CD8+T和CD4+CD25+Treg表达水平,实时荧光定量PCR法检测HBV DNA载量。多组间均数比较采用方差分析,进一步两两比较采用LSD-t检验,计数资料组间比较采用χ2检验;采用Pearson进行相关性分析。结果 CHB患者外周血CD4+CD25+Treg表达水平(30.97±18.78)%显著高于对照组(27.30±17.59)%,差异具有统计学意义(P<0.05);与对照组相比,CHB组、ASC组的CD3+T、CD4+T淋巴细胞百分数和CD4+与CD8+T淋巴细胞百分数比值显著降低,差异均有统计学意义(P...Abstract: Objective To investigate the correlation between the percentages of T lymphocyte subsets( CD3+T,CD4+T,CD8+T,and CD4+/ CD8+T) and CD4+CD25+regulatory T cells( Treg cells) and hepatitis B virus( HBV) DNA level in the peripheral blood of chronic hepatitis B( CHB) patients. Methods We chose 175 patients with HBV infection,who visited The Second People' s Hospital of Lanzhou from July to December,2012. The patients were divided into CHB group( n = 63) group and asymptomatic carrier( ASC) group( n =112). In the same period,50 healthy examinees served as control group. The percentages of CD3+,CD4+,CD8+,and CD4+/ CD8+T lymphocytes and CD4+CD25+Treg cells in the serum of three groups were measured by flow cytometry. HBV DNA level was analyzed by quantitative real- time PCR. The comparison of means between multiple groups was performed by analysis of variance. Further comparison between any two means was performed by LSD- t test. Correlation analysis was performed by Pearson's correlation test. Results The percentage of CD4+CD25+Treg( 30. 97% ± 18. 78%) in the peripheral blood of CHB patients was significantly higher than that of the control group( 27. 30% ± 17. 59%)( P < 0. 05). Compared with the control group,the CHB and ASC groups had significantly reduced percentages of CD3+and CD4+T lymphocytes and CD4+/ CD8+T lymphocyte ratio( P < 0. 05) and a significantly increased percentage of CD8+T lymphocytes( P < 0. 05). There was a positive correlation between the percentage of CD4+CD25+Treg cells and HBV DNA level in the ASC and CHG groups( r = 0. 501 and 0. 682,P < 0. 05 for both). Conclusion There are normal percentages of T lymphocyte subsets in HBV infection patients. HBV DNA can lead to an increased percentage of CD4+CD25+Treg cells,indicating that the imbalance of CD4+CD25+Treg and CD4+/ CD8+T cells plays an important role in the chronic progression of hepatitis B.
-
Key words:
- hepatitis B,chronic /
- T-lymphocytes,regulatory /
- T-lymphocyte subsets /
- viral buralen
期刊类型引用(16)
1. 巴明玉,潘研,王娴,陈亚琳,燕树勋. 强肝胶囊对非酒精性脂肪肝肥胖患者糖脂代谢水平及肝功能的影响. 中医学报. 2024(02): 416-420 . 百度学术
2. 范文,郑垂仰,周旋,孔怡琳,潘丰满,杨钦河,张玉佩. 6种中成药治疗非酒精性脂肪性肝病有效性和安全性的网状Meta分析. 中药新药与临床药理. 2023(11): 1612-1622 . 百度学术
3. 崔姗姗,刘英,江霞. 非酒精性脂肪性肝病与胰岛素抵抗的研究进展. 医学信息. 2022(10): 66-69 . 百度学术
4. 舒祥兵,操颖,顾晔,杨志新,季光,张莉. 强肝胶囊促进肝糖原合成降低大鼠高血糖的机制研究. 上海中医药大学学报. 2021(03): 45-51 . 百度学术
5. 贾英杰,张晓博,张新元,杨红霞,郭争荣. 强肝胶囊对猪血清诱导大鼠肝纤维化的治疗作用. 河北医药. 2021(20): 3104-3107 . 百度学术
6. 朱丹,孙婷婷,陈兰羽,谢铮. 脂肪性肝病中医药认识. 辽宁中医药大学学报. 2020(05): 70-73 . 百度学术
7. 崔翔,刘玲兰,华鹏,郭薇,薛敬东. 从脾论治非酒精性脂肪性肝病的体会与研究概况. 中医临床研究. 2020(14): 136-138 . 百度学术
8. 郭明升,刘娟,郭英杰,籍芳华,郝艳爽. 阿托伐他汀联用脂康颗粒或强肝胶囊用于脑梗死二级预防效果比较. 现代中西医结合杂志. 2020(22): 2400-2404+2446 . 百度学术
9. 王秀琴. 强肝胶囊联合甘草酸二铵肠溶胶囊治疗非酒精性脂肪肝的效果. 河南医学研究. 2019(06): 1089-1091 . 百度学术
10. 黄静,陈湘清,郭东升,吴奋. 同步抗感染治疗对合并幽门螺杆菌感染非酒精性脂肪肝病患者治疗结局的影响研究. 重庆医学. 2018(01): 97-100 . 百度学术
11. 陈少颖,易新宇,周小博,张荣臻. 中医药治疗非酒精性脂肪肝的研究进展. 大众科技. 2018(06): 68-70 . 百度学术
12. 周桃梅,杨上文,汪剑波. 强肝胶囊联合二甲双胍治疗非酒精性脂肪肝的疗效及其对Nod样受体-3炎症小体的影响. 中国医院统计. 2018(03): 206-208 . 百度学术
13. 柳琳琳,毛德文,吕建林,黄瑞. 强肝胶囊对非酒精性脂肪性肝病患者疗效及安全性的Meta分析. 中成药. 2018(08): 1715-1720 . 百度学术
14. 李滨,张佳圆,王凤永,李春晖,齐丽韫,王玉华,赵培利. 胆宁片治疗非酒精性脂肪肝临床效果及对血脂、肝功能和肝纤维化的影响. 解放军医药杂志. 2018(10): 93-96 . 百度学术
15. 朱紫馨,祝丽丽,程明亮. 同步抗感染治疗非酒精性脂肪肝合并幽门螺杆菌感染的效果观察. 临床合理用药杂志. 2018(31): 58-59 . 百度学术
16. 李军祥,王允亮,郭一,谢添弘. 中医药治疗非酒精性脂肪性肝病专题笔谈. 中国中西医结合消化杂志. 2017(11): 801-804 . 百度学术
其他类型引用(13)
-