成簇的规律间隔短回文重复序列/Cas9系统在病毒领域的应用

郑庆芬; 段钟平; 李建生

doi:10.3969/j.issn.1001-5256.2016.01.041

成簇的规律间隔短回文重复序列/Cas9系统在病毒领域的应用

DOI: 10.3969/j.issn.1001-5256.2016.01.041

1. 郑州大学第一附属医院
2. 首都医科大学附属北京佑安医院

基金项目:

科研基地建设-重大传染病防治协同创新中心(2011协同创新中心)(115215)； “艾滋病和病毒性肝炎等重大传染病防治”科技重大专项基金资助项目(2013ZX10002002-006)；北京市科学技术委员会资助临床特色项目(Z131107002213019;Z151100004015066)；

详细信息

中图分类号: R373
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出版历程
- 收稿日期: 2015-05-07
- 出版日期: 2016-01-20

Application of CRISPR/Cas9 system in virus research

The First Affiliated Hospital of Zhengzhou University

Research funding:

摘要

摘要: 目前成簇的规律间隔的短回文重复序列(CRISPR)/Cas9系统已经在很多物种及细胞中被广泛用来编辑基因组DNA,这一系统是第三代人工核酸内切酶,通过识别短的病毒序列来完成基因的编辑。介绍了该系统的结构特点以及在病毒领域的应用,包括病毒相关基因的功能研究,以及抗病毒治疗(包括HIV、HBV、EB病毒等)方面的研究,同时也对该系统未来在病毒方面的研究方向进行展望。
- 病毒 /
- RNA编辑 /
- 综述
Abstract: To date,the clustered regularly interspaced short palindromic repeat( CRISPR) / Cas9 system has been widely used to edit the genome in many species and cells. The system is the third generation of artificial endonuclease,which can edit DNA by recognizing short DNA sequences. This paper reviews the structural features of the system and its application in virus research,such as the functional studies of virus- related genes and the exploration of antiviral therapies( including HIV,HBV,and EB virus),looking forward to the future direction of virus research.
- virus /
- RNA editing /
- review

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参考文献(30)

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