Expression and clinical significance of astrocyte elevated gene-1,β-catenin,and cyclin D1 in hepatocellular carcinoma
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摘要: 目的探讨肝细胞癌(HCC)组织中星形细胞上调基因(AEG)1、β-连环素(β-catenin)及周期素D1(Cyclin D1)的表达及临床意义。方法随机选取2013年7月-2014年12月贵州省人民医院经手术及病理证实为HCC的癌组织标本和对应癌旁组织各40例,另选取8例正常肝组织作为对照组。采用免疫组化SP法检测AEG-1、β-catenin及Cyclin D1蛋白在HCC组织、对应癌旁肝组织及正常肝组织中的表达情况,并分析其表达与HCC临床病理因素的相关性。计数资料组间比较采用χ2检验或Fisher确切概率法,AEG-1、β-catenin、Cyclin D1在HCC中的相关性采用Spearman等级相关分析。结果 AEG-1、β-catenin及Cyclin D1在HCC组织及癌旁肝组织中的表达均高于正常肝组织,差异均有统计学意义(χ2值分别为7.840、4.274、8.817、4.274、9.919、4.850,P值分别为0.005、0.039、0.003、0.039、0.002、0.028)。AEG-1、β-catenin、Cyclin D1的阳性表达在性别、年龄、HBs ...Abstract: Objective To investigate the expression and clinical significance of astrocyte elevated gene- 1( AEG- 1),β- catenin,and cyclin D1 in hepatocellular carcinoma( HCC) tissues. Methods A total of 40 HCC samples and 40 samples of corresponding para- carcinoma tissues from the patients with pathologically confirmed HCC who underwent surgery in The People's Hospital of Guizhou from July 2013 to December 2014 were randomly selected,and 8 samples of normal liver tissues were selected as controls. The immunohistochemistry SP was used to measure the protein expression of AEG- 1,β- catenin,and cyclin D1 in HCC tissues,corresponding para- carcinoma tissues,and normal liver tissues,and the correlation between their expression and HCC clinicopathological characteristics was analyzed. The chi- square test or Fisher's exact test was used for comparison of categorical data between groups,and the Spearman rank correlation was used to analyze the correlation of AEG- 1 with β- catenin,and cyclin D1 in HCC. Results HCC tissues and para- carcinoma tissues showed significantly higher protein expression of AEG- 1,β- catenin,and cyclin D1 than normal liver tissues( χ2= 7. 840,4. 274,8. 817,4. 274,9. 919,and 4. 850,P = 0. 005,0. 039,0. 003,0. 039,0. 002,and 0. 028). The positive expression of AEG- 1,β- catenin,and cyclin D1 showed no significant differences across the patients with different sexes,ages,HBs Ag status,or tumor sizes( all P > 0. 05),but showed significant differences across the patients with different degrees of pathological differentiation,TNM stages for liver cancer,and metastases( all P < 0. 05). The correlation analysis showed that the protein expression of AEG- 1 was positively correlated with that of β- catenin and cyclin D1( r = 0. 420 and 0. 741,both P < 0. 01). Conclusion AEG- 1,β- catenin,and cyclin D1 may play vital roles in the development and progression of HCC. AEG- 1 may up- regulate the expression and activity of cyclin D1 and β- catenin and thus promote the development and metastasis of HCC. A combined measurement of AEG- 1,β- catenin,and cyclin D1 can be used as an important parameter for HCC gene therapy and prognostic evaluation.
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Key words:
- carcinoma /
- hepatocellular /
- astrocyte elevated gene 1 /
- beta catenin
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