慢性HBV感染不同阶段患者树突状细胞亚群的表达差异

陈伟霞; 王磊; 刘洋; 季光; 邢练军

doi:10.3969/j.issn.1001-5256.2017.03.012

慢性HBV感染不同阶段患者树突状细胞亚群的表达差异

DOI: 10.3969/j.issn.1001-5256.2017.03.012

1. 上海中医药大学
2. 上海中医药研究院脾胃病研究所
3. 上海中医药大学附属龙华医院脾胃病二科

基金项目:

国家自然科学基金项目(81302891)；上海市自然科学基金项目(14ZR1441600)；

详细信息

中图分类号: R512.62
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出版历程
- 出版日期: 2017-03-20

Expression of dendritic cell subsets in patients with different stages of chronic HBV infection:a comparative analysis

Shanghai University of Traditional Chinese Medicine

Research funding:

摘要

摘要: 目的探讨HBV相关疾病患者外周血树突状细胞亚群在不同病程中的变化及临床意义。方法选取上海中医药大学附属龙华医院肝病科HBV携带者（HBV携带组）、CHB患者（CHB组）、乙型肝炎肝硬化患者（肝硬化组）、原发性肝癌患者（肝癌组）（均为HBV相关疾病）及健康志愿者（正常组）各20例,采用流式细胞分析其外周血树突状细胞（DC）亚群的变化。多组间比较采用kruskal-wallis H检验,进一步两两比较采用Wilcoxon秩和检验。结果不同类型HBV感染者与正常人外周血髓样树突细胞（mDC）和浆细胞样树突细胞（pDC）比较结果显示,不同类型的HBV感染者与正常组之间m DC数量比较差异有统计学意义（χ2=33.629,P<0.001）,进一步比较发现肝硬化组、肝癌组患者外周血m DC的数量均低于正常组、CHB组和HBV携带组,差异均有统计学意义（P值均<0.05）;不同类型的HBV感染者与正常组之间pDC数量比较差异有统计学意义（χ2=13.602,P=0.009）,进一步比较发现肝癌组患者外周血pDC数量均低于正常组、CHB组和HBV...
- 肝炎,乙型,慢性 /
- 肝硬化 /
- 肝肿瘤 /
- 树突细胞
Abstract: Objective To investigate the changes in peripheral blood dendritic cell (DC) subsets in patients with different stages of hepatitis B virus (HBV) -associated disease and related clinical significance.Methods A total of 20 HBV carriers (HBV carrier group) , 20 chronic hepatitis B (CHB) patients (CHB group) , 20 hepatitis B cirrhosis patients (cirrhosis group) , and 20 primary liver cancer patients (liver cancer group) , all of whom had HBV-associated disease and were selected from Department of Hepatology, Longhua Hospital Affiliated to Shanghai University of Traditiond Chinese Medicine, as well as 20 healthy volunteers (normal group) , were included in this study flow cytometry was used to measure the changes in DC subsets.Comparison between multiple groups using Druskal-Wallis H test and further comparison using of Wilcoxon Rant test.Results According to the results of myeloid dendritic cells (mDCs) and plasmacytoid dendritic cells (pDCs) in patients with different types of HBV infection and normal population, there was a significant difference in the number of m DC between patients with different typers of HBV infection and normal population (χ2=33.629, P<0.001) , and the cirrhosis group and the liver cancer group had significantly lower numbers of mDCs in peripheral blood than the normal group, CHB group, and HBV carrier group (all P<0.05) .There was a significant difference in the number of pDC between patients with different types of HBV infection and normal population (χ2=13.602, P <0.009) , and the liver cancer group had a significantly lower number of pDCs in peripheral blood than the normal group, CHB group, and HBV carrier group (all P<0.05) .The cirrhosis group had a significantly lower number of pDCs in peripheral blood than the CHB group (P<0.05) .Conclusion There are significant differences in the expression of DC subsets between patients with different HBV-associated diseases, and the number of DCs may be used as a reference for evaluating disease progression and prognosis.
- hepatitis B /
- chronic /
- liver cirrhosis /
- liver neoplasms

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参考文献(13)

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