HBsAg定量在HBV感染自然史中的变化及意义

王慰; 戎君; 员美娜; 赵召霞; 唐亚芳; 高恒波

doi:10.3969/j.issn.1001-5256.2017.06.014

HBsAg定量在HBV感染自然史中的变化及意义

DOI: 10.3969/j.issn.1001-5256.2017.06.014

1. 石家庄市第五医院感染科
2. 河北医科大学

基金项目:

河北省科技成果重点推广计划(13277765D)；

详细信息

中图分类号: R512.62
计量
- 文章访问数: 2597
- HTML全文浏览量: 54
- PDF下载量: 512
- 被引次数: 0
出版历程
- 出版日期: 2017-06-20

Change in HBsAg quantification in the nature history of hepatitis B virus infection and its clinical significance

Research funding:

摘要

摘要: 目的观察慢性HBV感染后不同临床状态HBs Ag定量分布特点及其与HBV DNA水平的相关性。方法收集2013年1月-2015年12月石家庄市第五医院的268例未经抗病毒治疗的HBV感染者进行横断面研究。依据HBV感染的不同时期分为4组,免疫耐受期29例、免疫清除期169例、低（非）复制期19例、再活动期51例。采用罗氏电化学发光法检测血清HBs Ag定量,PCR检测HBV DNA水平,并对HBs Ag和HBV DNA结果进行对数转换;对不同时期的结果进行统计分析。多组分析采用KruskalWallis H秩和检验,进一步两两比较采用Mann-Whitney U检验,相关性分析采用Spearman秩相关检验。结果 HBV感染的不同时期HBs Ag定量水平差异有统计学意义（χ2=71.830,P<0.001）,免疫耐受期HBs Ag定量显著高于其他3期（P值均<0.05）;免疫清除期HBs Ag定量显著高于低复制期和再活动期（P值均<0.05）。HBV DNA水平在各组间差异有统计学意义（χ2=117.028,P<0.001）,免疫耐受期HBV DNA水平显著高于...
- 肝炎,乙型,慢性 /
- 肝炎表面抗原,乙型 /
- 感染
Abstract: Objective To investigate the distribution characteristics of HBsAg quantification in different clinical status after chronic hepatitis B virus (HBV) infection and its correlation with HBV DNA level. Methods A cross-sectional study was performed for 268 patients with HBV infection who did not receive antiviral therapy in The Fifth Hospital of Shijiazhuang from January 2013 to December 2015. According to the stage of HBV infection, these patients were divided into immune tolerance group with 29 patients, immune clearance group with 169 patients, low-or non-replication group with 19 patients, and reactivation group with 51 patients. Roche electrochemical luminescence was used to measure serum HBsAg quantification and PCR was used to measure HBV DNA level. Logarithmic transformation was performed for the results of HBsAg quantification and HBV DNA level, and a statistical analysis was performed for the results of different stages. The Kruskal-Wallis H rank sum test was used for the analysis of multiple groups, and the Mann-Whitney U test was used for further comparison between any two groups; a Spearman rank correlation analysis was also performed. Results HBsAg quantification varied in different stages of HBV infection (χ2= 71. 830, P < 0. 001) ; the immune tolerance group had significantly higher HBsAg quantification than the other three groups (all P < 0. 05) , and the immune clearance group had significantly higher HBsAg quantification than the low-replication group and the reactivation group (all P < 0. 05) . There was a significant difference in HBV DNA level between groups (χ2= 117. 028, P <0. 001) ; the immune tolerance group had a significantly higher HBV DNA level than the other three groups (all P < 0. 05) , the immune clearance group had a significantly higher HBV DNA level than the low-replication group and the reactivation group (all P < 0. 05) , and the reactivation group had a significantly higher HBV DNA level than the low-replication group (both P < 0. 05) . HBsAg quantification was correlated with HBV DNA level in immune clearance stage (r = 0. 559, P < 0. 001) and reactivation stage (r = 0. 338, P = 0. 014) . Conclusion HBsAg quantification and HBV DNA level in immune tolerance stage are higher than those in the other three stages, and HBsAg quantification is correlated with HBV DNA level in immune clearance stage and reactivation stage.
- hepatitis B, chronic /
- hepatitis B surface antigens /
- infection

HTML全文

参考文献(9)

[1]Chinese Society of Hepatology and Chinese Society of Infectious Diseases, Chinese Medical Association.The guideline of prevention and treatment for chronic hepatitis B:a 2015 update[J].J Clin Hepatol, 31 (12) :1941-1960. (in Chinese) 中华医学会肝病学分会, 中华医学会感染病学分会.慢性乙型肝炎防治指南 (2015年更新版) [J].临床肝胆病杂志, 2015, 31 (12) :1941-1960.

[2]ZENG QL, WANG FS.Fluctuating characteristics of HBs Ag quantification and immune system in patients with chronic hepatitis B[J].Chin J Hepatol, 2012, 20 (10) :726-729. (in Chinese) 曾庆磊, 王福生.慢性乙型肝炎患者HBs Ag定量与机体免疫系统的变化特点[J].中华肝脏病杂志, 2012, 20 (10) :726-729.

[3]CHAN HL, WONG VW, WONG GL, et al.A longitudinal study on the natural history of serum hepatitis B surface antigen changes in chronic hepatitis B[J].Hepatology, 2010, 52 (4) :1232-1241.

[4] FU WZ, YANG L, HU XQ.Relationship between HBV DNA load and HBV-M, ALT, AST content in the serum of chronic hepatitis B patients[J].Int J Virol, 2015, 22 (2) :111-114. (in Chinese) 付万智, 杨丽, 胡晓勤.慢性乙肝患者血清HBV DNA载量与HBV-M、ALT、AST含量的关系研究[J].国际病毒学杂志, 2015, 22 (2) :111-114.

[5]JAROSZEWICZ J, SERRANO BC, WURSTHORN K, et al.Hepatitis B surface antigen levels in the natural history of hepatitis B virus infection:a European perspective[J].J Hepatol, 2010, 52 (4) :514-522.

[6]YANG JH, JIA W, WANG YE, et al.Hepatitis B virus infection in different clinical serum HBs Ag levels during the period of comparison[J].Chin Hepatol, 2013, 18 (11) :749-751. (in Chinese) 杨建华, 贾雯, 汪月娥, 等.乙型肝炎病毒感染者不同临床时期血清HBs Ag水平的比较[J].肝脏, 2013, 18 (11) :749-751.

[7]ZHU ST, XIE QX, ZHANG YF, et al.Detection of the covalently closed circular DNA in peripheral blood mononuclear cells of hepatitis B patients and its clinical significance[J].Chin J Infect Dis, 2014, 32 (4) :214-218. (in Chinese) 朱圣涛, 谢琴秀, 张亚飞, 等.乙型肝炎患者外周血单个核细胞中共价闭合环状DNA的检测及其临床意义[J].中华传染病杂志, 2014, 32 (4) :214-218.

[8]JIANG J, CHEN SX, XU WS, et al.Quantitative analysis of intrahepatic covalently closed circular hepatitis B virus DNA in hepatitis B virus infected patients with normal liver function[J].Chin J Infect Dis, 2013, 31 (8) :458-461. (in Chinese) 江军, 陈仕祥, 徐文胜, 等.肝功能正常的乙型肝炎病毒感染者肝组织共价闭合环状DNA的研究[J].中华传染病杂志, 2013, 31 (8) :458-461.

[9]MELLERGRAD P, SJGREN F, HILLMAN J.The cerebral extracellularrelease of glycerol, glycerol, glutamate, and FGF2 is increased in older patients following severe traumatic brain injury[J].J Neurotrauma, 2012, 29 (1) :112-128.

施引文献

资源附件(0)

访问统计