干细胞移植治疗慢加急性肝衰竭的研究进展

肖笑枭; 梁锐

doi:10.3969/j.issn.1001-5256.2017.08.036

干细胞移植治疗慢加急性肝衰竭的研究进展

DOI: 10.3969/j.issn.1001-5256.2017.08.036

肖笑枭,
梁锐

1. 云南中医学院
2. 云南省第一人民医院病理科

基金项目:

云南省应用基础计划项目(2013FZ179、2014FB040)；国家自然科学基金(81660302)；

详细信息

中图分类号: R575.3
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- 被引次数: 0
出版历程
- 收稿日期: 2017-01-25
- 出版日期: 2017-08-20

Research advances in stem cell transplantation for the treatment of acute-on-chronic liver failure

Yunnan University of Traditional Chinese Medicine

Research funding:

摘要

摘要: 慢加急性肝衰竭(ACLF)是临床上的危重疾病,病死率高达80%。目前临床对于ACLF仍缺乏理想的治疗措施。干细胞移植技术治疗ACLF具有其独特的优势,但现阶段关于该技术治疗ACLF的研究仍存在一些问题,因此导致其临床应用受到了限制。从传统治疗手段、干细胞来源、移植途径以及所存在的问题等4个方面对干细胞移植治疗ACLF进行阐述,以期对未来相关研究有所帮助。
- 肝功能衰竭 /
- 干细胞移植 /
- 治疗 /
- 综述
Abstract: Acute-on-chronic liver failure ( ACLF) is a critical disease in clinical practice and has a mortality rate as high as 80%. At present, there are still no ideal therapeutic measures for ACLF. Stem cell transplantation has unique advantages in the treatment of ACLF;however, there are still some issues regarding the research on stem cell transplantation, which limit its clinical application. This article elaborates on conventional treatment methods, source of stem cells, transplantation approach, and related issues, in order to provide help for further studies.
- liver failure /
- stem cell transplantation /
- therapy /
- review

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参考文献(50)

[1]CRAIG DG, BATES CM, DAVIDSON JS, et al.Overdose pattern and outcome in paracetamol-induced acute severe hepatotoxicity[J].Br J Clin Pharmacol, 2011, 71 (2) :273-282.

[2]LI XD, XU DP.Immunopathogenesis of acute-on-chronic hepatitis B liver failure[J].J Pract Hepatol, 2015, 18 (3) :317-320. (in Chinese) 李晓东, 徐东平.HBV相关慢加急性肝衰竭免疫病理发病机制研究进展[J].实用肝脏病杂志, 2015, 18 (3) :317-320.

[3]GUSTOT T.Beneficial role of G-CSF in acute-on-chronic liver failure:effects on liver regeneration, inflammation/immunoparalysis or both?[J].Liver Int, 2014, 34 (4) :484-486.

[4]LIU H, CHEN G, GAN XM.Research advances in new approaches to treatment of acute-on-chronic liver failure[J].J Clin Hepatol, 2015, 31 (9) :1496-1500. (in Chinese) 刘洪, 陈刚, 甘雪梅.慢加急性肝衰竭治疗的新技术及其研究进展[J].临床肝胆病杂志, 2015, 31 (9) :1496-1500.

[5]Liver Failure and Artificial Liver Group, Chinese Society of Infectious Diseases, Chinese Medical Association;Severe Liver Diseases and Artificial Liver Group, Chinese Society of Hepatology, Chinese Medical Association.Diagnostic and treatment guidelines for liver failure[J].Chin J Hepatol, 2006, 14 (9) :643-646. (in Chinese) 中华医学会感染病学分会肝衰竭与人工肝学组, 中华医学会肝病学分会重型肝病与人工肝学组.肝衰竭诊疗指南[J].中华肝脏病杂志, 2006, 14 (9) :643-646.

[6]LI F.Clinical effect of artificial liver plasma exchange in treatment of acute-on-chronic liver failure:an analysis of 60 cases[J].J Pract Med Tech, 2016, 23 (10) :1112-1113. (in Chinese) 李芳.人工肝血浆置换术治疗慢加急性肝衰竭60例临床疗效观察[J].实用医技杂志, 2016, 23 (10) :1112-1113.

[7] LI MQ.Clinical effect of non-bioartificial liver in treatment of liver failure with multiple organ dysfunction syndrome[C]//China Society of Integrated Traditional Chinese and Western Medicine, Expert Committee on Disaster Medicine in China Society of Integrated Traditional Chinese and Western Medicine, Jiangsu Association of Integrated Traditional Chinese and Western Medicine.Proceedings of The 10th Academic Conference on Integrated Traditional Chinese and Western Medicine and Disaster Medicine, Founding Conference for Expert Committees on Disaster Medicine and Critical Care Medicine, and Exhibition and Negotiation Conference for Achievements in Health Industry, 2014:2. (in Chinese) 李茂琴.非生物型人工肝治疗重症肝功能衰竭合并MODS临床研究[C]//中国中西医结合学会、中国中西医结合学会灾害医学专业委员会、江苏省中西医结合学会.2014第十届全国中西医结合灾害医学学术大会江苏省中西医结合学会灾害医学、重症医学专业委员会成立大会暨健康产业成果展示洽谈会学术论文集, 2014:2.

[8]DUAN BW, LU SC, WU JS, et al.Model for End-Stage Liver Disease (MELD) score does not predict outcomes of hepatitis Binduced acute-on-chronic liver failure in transplant recipients[J].Transplant Proc, 2014, 46 (10) :3502-3506.

[9]LEVITSKY J, O'LEARY JG, ASRANI S, et al.Protecting the kidney in liver transplant recipients:practice-based recommendations from the american society of transplantation liver and intestine community of practice[J].Am J Transplant, 2016, 16 (9) :2532-2544.

[10]ZHENG SS, YU J, ZHANG W.Current development of liver transplantation in China[J].J Clin Hepatol, 2014, 30 (1) :2-4. (in Chinese) 郑树森, 俞军, 张武.肝移植在中国的发展现状[J].临床肝胆病杂志, 2014, 30 (1) :2-4.

[11]LI YR, LIU ZW, LYU JH, et al.Hepatocyte transplantation and its current research progress[J].Mil Med Sci, 2012, 36 (2) :154-157, 161. (in Chinese) 李育蓉, 刘振文, 吕金辉, 等.肝细胞移植的研究进展[J].军事医学, 2012, 36 (2) :154-157, 161.

[12]TAKAMI T, TERAI S, SAKAIDA I, et al.Stem cell therapy in chronic liver disease[J].Curr Opin Gastroenterol, 2012, 28 (3) :203-208.

[13]WILSON JW, LEDUC EH.Role of cholangioles in restoration of the liver of the mouse after dietary injury[J].J Pathol Bacteriol, 1958, 76 (2) :441-449.

[14]FARBER E.Similarities in the sequence of early histological changes induced in the liver of the rat by ethionine, 2-acetylaminofluorene, and 3'-methyl-4-dimethylaminoazobenzene[J].Cancer Res, 1956, 16 (2) :142-148.

[15]YUAN D, LIN G, LU GX.Differentiation of neuroepithelial progenitor cells from human embryonic stem cells[J].Life Sci Res, 2010, 14 (2) :95-99. (in Chinese) 袁丁, 林戈, 卢光琇.人胚胎干细胞向神经上皮祖细胞的诱导分化[J].生命科学研究, 2010, 14 (2) :95-99.

[16]FRANCIS KR, WEI L.Human embryonic stem cell neural differentiation and enhanced cell survival promoted by hypoxic preconditioning[J].Cell Death Dis, 2010, 1 (2) :e22.

[17]FUJIWARA M, YAN P, OTSUJI TG, et al.Induction and enhancement of cardiac cell differentiation from mouse and human induced pluripotent stem cells with cyclosporin-A[J].PLo S One, 2011, 6 (2) :e16734.

[18]LI XD, WANG JW, WEI GF.Human embryonic stem cells maintained and cultured in serum-free medium m Te SR (r) 1 and induced into endothelial cells differentiation[J].J Clin Rehabil Tissue Eng Res, 2011, 15 (10) :1827-1831. (in Chinese) 李向东, 王纪文, 魏国峰.人胚胎干细胞在无血清m Te SR (r) 1培养基中维持培养和向内皮细胞的诱导分化[J].中国组织工程研究与临床康复, 2011, 15 (10) :1827-1831.

[19]YAMADA T, YOSHIKAWA M, KANDA S, et al.In vitro differentiation of embryonic stem cells into hepatocyte-like cells identified by cellular uptake of indocyanine green[J].Stem Cells, 2002, 20 (2) :146-154.

[20]RAMBHATLA L, CHIU CP, KUNDU P, et al.Generation of hepatocyte-like cells from human embryonic stem cells[J].Cell Transplant, 2003, 12 (1) :1-11.

[21]YAMAMOTO H, QUINN G, ASARI A, et al.Differentiation of embryonic stem cells into hepatocytes:biological functions and therapeutic application[J].Hepatology, 2003, 37 (5) :983-993.

[22]LAGASSE E, CONNORS H, AL-DHALIMY M, et al.Purified hematopoietic stem cells can differentiate into hepatocytes in vivo[J].Nat Med, 2000, 6 (11) :1229-1234.

[23]LI W, WANG L, ZENG J, et al.Effect of cholestatic serum on differentiation of bone marrow mesenchymal stem cells into hepatocytes[J].J Clin Rehabil Tissue Eng Res, 2016, 20 (6) :771-776. (in Chinese) 李伟, 汪林, 曾杰, 等.瘀胆血清诱导骨髓间充质干细胞向肝细胞的分化[J].中国组织工程研究, 2016, 20 (6) :771-776.

[24]WAGERS AJ, SHERWOOD RI, CHRISTENSEN JL, et al.Little evidence for developmental plasticity of adult hematopoietic stem cells[J].Science, 2002, 297 (5590) :2256-2259.

[25]MONSEL A, ZHU YG, GENNAI S, et al.Cell-based therapy for acute organ injury:preclinical evidence and ongoing clinical trials using mesenchymal stem cells[J].Anesthesiology, 2014, 121 (5) :1099-1121.

[26]VOLAREVIC V, NURKOVIC J, ARSENIJEVIC N, et al.Concise review:therapeutic potential of mesenchymal stem cells for the treatment of acute liver failure and cirrhosis[J].Stem Cells, 2014, 32 (11) :2818-2823.

[27]BISHI DK, MATHAPATI S, CHERIAN KM, et al.In vitro hepatic trans-differentiation of human mesenchymal stem cells using sera from congestive/ischemic liver during cardiac failure[J].PLo S One, 2014, 9 (3) :e92397.

[28]LI B, LIU Y, YANG P.Advances in the application of mesenchymal stem cells[J].Chin J Med Offic, 2016, 44 (2) :205-208. (in Chinese) 李波, 刘杨, 杨鹏.间充质干细胞应用进展[J].临床军医杂志, 2016, 44 (2) :205-208.

[29]ZHAO MZ, WANG DD, YU ZJ, et al.Promotive effect of hepatocellular inj ury-conditioned medium on hepatic differentiation of bone marrow mesenchymal stem cells in rats[J].J Jilin Univ:Med Edit, 2016, 42 (6) :1108-1115. (in Chinese) 赵美子, 王丹丹, 虞振静, 等.肝细胞损伤条件培养基对大鼠骨髓间充质干细胞肝向分化的促进作用[J].吉林大学学报:医学版, 2016, 42 (6) :1108-1115.

[30]CHEN YH, CHANG MH, CHIEN CS, et al.Contribution of mature hepatocytes to small hepatocyte-like progenitor cells in retrorsine-exposed rats with chimeric livers[J].Hepatology, 2013, 57 (3) :1215-1224.

[31]WU QW, ZHANG DQ, HU HQ, et al.Research progress and application prospects of liver stem cell transplantation[J].Sichuan J Physiol Sci, 2015, 37 (3) :139-142. (in Chinese) 吴雄伟, 张丹琴, 胡惠琼, 等.肝干细胞移植的研究进展及其应用前景[J].四川生理科学杂志, 2015, 37 (3) :139-142.

[32]CHEN LP, YE H.Research advances in hepatocyte transplantation in treatment of acute liver failure[J].Sichuan Med, 2012, 33 (5) :892-894. (in Chinese) 陈利平, 叶辉.肝细胞移植治疗急性肝衰竭的研究进展[J].四川医学, 2012, 33 (5) :892-894.

[33]OKUMOTO K, SAITO T, HATTORI E, et al.Expression of Notch signalling markers in bone marrow cells that differentiate into a liver cell lineage in a rat transplant model[J].Hepatol Res, 2005, 31 (1) :7-12.

[34]ZHANG LX, LI Y, WANG LM, et al.Bone marrow mesenchymal stem cell transplantation via different approaches in treatment of liver cirrhosis in mice[J].J Clin Hepatol, 2016, 32 (10) :1906-1910. (in Chinese) 张丽霞, 李莹, 王黎明, 等.不同途径移植骨髓间充质干细胞治疗肝硬化小鼠的效果比较[J].临床肝胆病杂志, 2016, 32 (10) :1906-1910.

[35]HAN JP, CHEN Z.Study on mesenchymal stem cells transplantation through the spleen in treatment of acute liver failure in rat[J].Chin J Bases Clin Gen Surg, 2014, 21 (6) :669-675. (in Chinese) 韩建平, 陈钟.骨髓间充质干细胞经脾移植治疗大鼠急性肝功能衰竭的实验研究[J].中国普外基础与临床杂志, 2014, 21 (6) :669-675.

[36]GUO XL, LIU Y, ZHOU LM, et al.Adipose-derived mesenchymal stem cell transplantation via the hepatic artery for the treatment of advanced liver diseases[J].J Clin Rehabil Tissue Eng Res, 2016, 20 (6) :848-854. (in Chinese) 郭宪立, 刘跃, 周利敏, 等.脂肪间充质干细胞经肝动脉移植治疗晚期肝病[J].中国组织工程研究, 2016, 20 (6) :848-854.

[37]WANG F, ZHOU L, MA X, et al.Monitoring of intrasplenic hepatocyte transplantation for acute-on-chronic liver failure:a prospective five-year follow-up study[J].Transplant Proc, 2014, 46 (1) :192-198.

[38]AHMAD TA.Study of the survival of hepatocytes transplanted to the spleen in rats[J].Hapetogastroenterology, 2011, 58 (107-108) :892-895.

[39]OUYANG S, LIU SR, CHENG T, et al.Hepatic arterial transplantation of autologous bone marrow mesenchymal stem cells in treatment of decompensated liver cirrhosis[J].J Clin Rehabil Tissue Eng Res, 2013, 17 (36) :6455-6461. (in Chinese) 欧阳石, 刘树人, 程涛, 等.自体骨髓间充质干细胞肝动脉移植治疗失代偿期肝硬化[J].中国组织工程研究, 2013, 17 (36) :6455-6461.

[40]HUANG XL, LUO L, LUO LY, et al.Clinical outcome of autologous hematopoietic stem cell infusion via hepatic artery or portal vein in patients with end-stage liver diseases[J].Chin Med Sci J, 2014, 29 (1) :15-22.

[41]BARBASH IM, CHOURAQUI P, BZRON J, et al.Systemic delivery of bone marrow-derived mesenchymal stem cells to the infarcted myocardium:feasibility, cell migration, and body distribution[J].Circulation, 2003, 108 (7) :863-868.

[42]CHO KA, LIM GW, JOO SY, et al.Transplantation of bone marrow cells reduces CCl4-induced liver fibrosis in mice[J].Liver Int, 2011, 31 (7) :932-939.

[43]WHO.Hepatitis B[EB/OL].[2016-07].http://www.who.int/mediacentre/factsheets/fs204/en/

[44]STENBG P, BUSK T, LARSEN FS.Efficacy of liver assisting in patients with hepatic encephalopathy with special focus on plasma exchange[J].Metab Brain Dis, 2013, 28 (2) :333-335.

[45]YE JL, YE B, LV JX, et al.Changes of ammonia levels in patients with acute on chronic liver failure treated by plasma exchange[J].Hepatogastroenterology, 2014, 61 (129) :141-145.

[46]LIU J, RUAN B, YOU N, et al.Downregulation of miR-200a induces EMT phenotypes and CSC-like signatures through targeting theβ-catenin pathway in hepatic oval cells[J].PLo S One, 2013, 8 (11) :e79409.

[47]FACCIORUSSO A, ANTONINO M, DEL PRETE V, et al.Are hematopoietic stem cells involved in hepatocarcinogenesis?[J].Hepatobiliary Surg Nutr, 2014, 3 (4) :199-206.

[48]PUSTERLA T, NMETH J, STEIN I, et al.Receptor for advanced glycation endproducts (RAGE) is a key regulator of oval cell activation and inflammation-associated liver carcinogenesis in mice[J].Hepatology, 2013, 58 (1) :363-373.

[49]MICHISHITA M, EZAKI S, OGIHARA K, et al.Identification of tumor-initiating cells in a canine hepatocellular carcinoma cell line[J].Res Vet Sci, 2014, 96 (2) :315-322.

[50]YAMASHITA T, HONDA M, NAKAMOTO Y, et al.Discrete nature of Ep CAM+and CD90+cancer stem cells in human hepatocellular carcinoma[J].Hepatology, 2013, 57 (4) :1484-1497.

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