核因子-κB p65在肝细胞癌中的表达及意义

夏庚; 祁飞; 杨晋辉; 唐映梅; 石梦琳; 张峻崎

doi:10.3969/j.issn.1001-5256.2017.12.017

核因子-κB p65在肝细胞癌中的表达及意义

DOI: 10.3969/j.issn.1001-5256.2017.12.017

昆明医科大学第二附属医院消化内科二病区

详细信息

中图分类号: R735.7
计量
- 文章访问数: 2349
- HTML全文浏览量: 17
- PDF下载量: 439
- 被引次数: 7
出版历程
- 收稿日期: 2017-06-15
- 出版日期: 2017-12-20

Expression of nuclear factor-kappa B p65 in hepatocellular carcinoma and its significance

Second Disease Area, Department of Gastroenterology, The Second Affiliated Hospital of Kunming Medical University

摘要

摘要: 目的利用肝癌组织芯片观察核因子-κB（NF-κB）p65在癌组织及癌旁组织中的表达,探讨其对肝癌发生、发展的意义。方法肝癌组织芯片（HLiv H180Su05）包括81例癌组织和81例癌旁组织,手术时间为2006年5月-2007年5月,随访截止时间为2012年2月,其中复发46例,无复发35例;参考美国癌症联合会胆囊癌TNM分期（第7版）分为:Ⅰ期56例,Ⅱ期25例;有癌栓28例,无癌栓53例。利用免疫组化法检测NF-κB P65蛋白的表达及相关性。依据所检测蛋白在细胞内的定位,分别以检测蛋白表达部位的阳性信号强度和阳性表达细胞数2种观察标准进行综合评分。计数资料组间比较采用χ2检验,采用Kaplan-Meier法进行生存分析,采用log-rank时序检验比较生存率。结果 NF-κB p65蛋白在癌组织及癌旁组织表达定位于细胞质及部分细胞核,在癌组织及癌旁组织阳性率分别为90.12%、69.14%,差异有统计学意义（χ2=10.998,P=0.001）;癌组织中NF-κB p65蛋白表达与临床分期、癌栓、有无复发无明显相关性（P值均>0.05）。结论 NF-κB p65在肝癌组织...
- 癌,肝细胞 /
- NF-κB /
- 转录因子RelA
Abstract: Objective To investigate the expression of nuclear factor-kappa B ( NF-κB) p65 in cancer tissue and adjacent tissue using hepatoma tissue microarray, as well as its significance in the development and progression of hepatocellular carcinoma ( HCC) . Methods The hepatoma tissue microarray HLiv H180 Su05 was used to analyze 81 cancer tissue samples and 81 adjacent tissue samples. Surgery was performed from May 2006 to May 2007, and the patients were followed up to February 2012. Of all patients, 46 experienced recurrence and35 did not experience recurrence. According to the TNM staging of gallbladder carcinoma developed by the American Joint Committee on Cancer, 56 had stage I tumor and 25 had stage II tumor; 28 had tumor thrombus and 53 had no tumor thrombus. Immunohistochemistry was used to measure the expression of NF-κB p65, and its association with other factors was analyzed. On the basis of the localization of the protein to be measured, a comprehensive evaluation was performed based on the intensity of positive signal and the number of positive cells.The chi-square test was used for comparison of categorical data between groups, the Kaplan-Meier method was used for survival analysis, and the log-rank test was used for the comparison of survival rates. Results NF-κB p65 protein was located in the cytoplasm and some nuclei in cancer tissue and adjacent tissue, with positive rates of 90. 12% in the cancer tissue and 69. 14% in the adjacent tissue, and there was a significant difference in positive rate between the cancer tissue and the adjacent tissue ( χ2= 10. 998, P = 0. 001) . The expression of NF-κB p65 protein in cancer tissue was not associated with clinical stage, tumor thrombus, or the presence or absence of recurrence ( all P > 0. 05) . Conclusion HCC tissue has higher expression of NF-κB p65 than adjacent tissue. The key to the regulation of NF-κB function is to inhibit the promotional effect of Rel A ( p65) on liver tumor. Therefore, inhibiting the activity of NF-κB p65 may suppress the differentiation and growth of tumor cells, providing a new idea for clinical treatment of HCC.
- carcinoma, hepatocellular /
- NF-kappa B /
- transcription factor RelA

HTML全文

参考文献(16)

[1]FERLAY J, SHIN HR, BRAY F, et al.Estimates of worldwide burden of cancer in 2008:GLOBOCAN2008[J].Int J Cancer, 2010, 127 (12) :2893-2917.

[2]ZHAO RR, DENG YD, YUAN H.Epidemiological and clinical features of primary liver cancer:an analysis of 236 patients[J].JClin Hepatol, 2016, 32 (8) :1538-1542. (in Chinese) 赵荣荣, 邓永东, 袁宏.236例原发性肝癌患者流行病学及临床特点分析[J].临床肝胆病杂志, 2016, 32 (8) :1538-1542.

[3]SEN R, BALTIMORE D.Multiple nuclear factors interact with the immunoglobulin enhancer sequences[J].Cell, 1986, 46 (5) :705-716.

[4]EDGE SB, BYRD DR, COMPTON CC, et al.AJCC cancer staging manual[M].7th ed.New York:Springer, 2009:211-217.

[5]YANG XD, YANG GP, HUANG J, et al.Regulatory mechanisms of NF-κB on cytochrome P450 enzymes[J].Chin J Clin Pharmacol Ther, 2015, 11 (20) , 1302-1307. (in Chinese) 阳喜定, 阳国平, 黄洁, 等.NF-κB对细胞色素P450调控作用机制研究进展[J].中国临床药理学与治疗学, 2015, 20 (11) :1302-1307.

[6]SUN DN, HUANG X.Roles and mechanism of NF-κB in hepatocellular carcinoma development[J].Chin Med Herald, 2016, 13 (18) :42-45. (in Chinese) 孙丹妮, 黄洵.NF-κB在肝细胞癌发生发展中的作用及其机制研究进展[J].中国医药导报, 2016, 13 (18) :42-45.

[7]HAN HB, LIANG NZ, ZHOU YP, et al.Expression and clinical significanceof NF-κB and COX-2 in colorectal cancer[J].J Clin Exp Med, 2015, 14 (16) :1342-1344. (in Chinese) 韩宏斌, 梁宁震, 周怡萍, 等.NF-κB、COX-2在结直肠癌组织中的表达及其临床意义[J].临床和实验医学杂志, 2015, 14 (16) :1342-1344.

[8]ZHANG M, DONG X, ZHANG D, et al.High expression of snail and NF-κB predicts poor survival in Chinese hepatocellular carcinoma patients[J].Oncotarget, 2017, 8 (3) :4543-4548.

[9]WU LQ, GE HP, WU JB.The expression of NF-κB protein in hepatocellular carcinoma[J].Clin Med J China, 2007, 14 (1) :17-18. (in Chinese) 邬林泉, 戈华平, 吴金宝.NF-κB在肝细胞肝癌组织中的表达及其意义[J].中国临床医学杂志, 2007, 14 (1) :17-18.

[10]HUBER MA, AZOITEI N, BAUMANN B, et al.NF-κB is essential for epithelial-mesenchymal transition and metastasis in a model of breast cancer progression[J].J Clin Investig, 2004, 114 (4) :569-581.

[11]HE M, ZHANG W, DONG Y, et al.Pro-inflammation NF-κB signaling triggers a positive feedback via enhancing cholesterol accumulation in liver cancer cells[J].J Exp Clin Cancer Res, 2017, 36 (15) :1-13.

[12]JI Y, WANG Z, LI Z, et al.Angiotensin II enhances proliferation and inflammation through AT1/PKC/NF-κB signaling pathway in hepatocellular carcinoma cells[J].Cell Physiol Biochem, 2016, 39 (1) :13-32.

[13]CAO W, HU C, WU L, et al.Rosmarinic acid inhibits inflammation and angiogenesis of hepatocellular carcinoma by suppression of NF-κB signaling in H22 tumor-bearing mice[J].J Pharmacol Sci, 2016, 132 (2) :131-137.

[14]LI M, ZHANG M, ZHANG ZL, et al.Induction of apoptosis by berberine in hepatocellular carcinoma Hep G2 cells via downregulation of NF-κB[J].Oncol Res, 2017, 25 (2) :233-239.

[15]SHINODA K, KUBOKI S, SHIMIZU H, et al.Pin1 facilitates NF-κB activation and promotes tumour progression in human hepatocellular carcinoma[J].Br J Cancer, 2015, 113 (9) :1323-1331.

[16]MOLES A, BUTTERWORTH JA, SANCHEZ A, et al.A Rel A (p65) Thr505 phospho-site mutation reveals an important mechanism regulating NF-κB-dependent liver regeneration and cancer[J].Oncogene, 2016, 35 (35) :4623-4632.

施引文献

期刊类型引用(6)

1.	严兆兰. 青海地区乙型肝炎肝硬化并发轻微肝性脑病Stroop测试及诱发疾病危险因素调查分析. 中华养生保健. 2024(22): 84-86 . 百度学术
2.	符健，高艺，林锋，李盼盼. 海口地区轻微肝性脑病PHES标准参考值范围的研究. 海南医学. 2023(13): 1903-1907 . 百度学术
3.	樊亚楠，纪童童，梁荣月，于岩岩，徐京杭. 肝硬化合并显性肝性脑病危险因素的回顾性分析：一项单中心病例对照研究. 中华肝脏病杂志. 2021(02): 133-136 . 百度学术
4.	边倩，解新科，寇小妮，李京涛，魏海梁，闫曙光. 通腑降浊汤联合乳果糖治疗热扰心神型肝性脑病临床观察. 山东中医杂志. 2021(06): 577-582 . 百度学术
5.	覃秀容，陈月桥，石清兰，毛德文. 轻微肝性脑病的中西医研究进展. 中医药导报. 2020(12): 152-156 . 百度学术
6.	王琳. 疏肝解郁胶囊治疗伴抑郁焦虑的肝性脑病患者的效果. 国际精神病学杂志. 2019(05): 925-928 . 百度学术