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Expression of nuclear factor-kappa B p65 in hepatocellular carcinoma and its significance
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摘要: 目的利用肝癌组织芯片观察核因子-κB（NF-κB）p65在癌组织及癌旁组织中的表达,探讨其对肝癌发生、发展的意义。方法肝癌组织芯片（HLiv H180Su05）包括81例癌组织和81例癌旁组织,手术时间为2006年5月-2007年5月,随访截止时间为2012年2月,其中复发46例,无复发35例;参考美国癌症联合会胆囊癌TNM分期（第7版）分为:Ⅰ期56例,Ⅱ期25例;有癌栓28例,无癌栓53例。利用免疫组化法检测NF-κB P65蛋白的表达及相关性。依据所检测蛋白在细胞内的定位,分别以检测蛋白表达部位的阳性信号强度和阳性表达细胞数2种观察标准进行综合评分。计数资料组间比较采用χ2检验,采用Kaplan-Meier法进行生存分析,采用log-rank时序检验比较生存率。结果 NF-κB p65蛋白在癌组织及癌旁组织表达定位于细胞质及部分细胞核,在癌组织及癌旁组织阳性率分别为90.12%、69.14%,差异有统计学意义（χ2=10.998,P=0.001）;癌组织中NF-κB p65蛋白表达与临床分期、癌栓、有无复发无明显相关性（P值均>0.05）。结论 NF-κB p65在肝癌组织...Abstract: Objective To investigate the expression of nuclear factor-kappa B ( NF-κB) p65 in cancer tissue and adjacent tissue using hepatoma tissue microarray, as well as its significance in the development and progression of hepatocellular carcinoma ( HCC) . Methods The hepatoma tissue microarray HLiv H180 Su05 was used to analyze 81 cancer tissue samples and 81 adjacent tissue samples. Surgery was performed from May 2006 to May 2007, and the patients were followed up to February 2012. Of all patients, 46 experienced recurrence and35 did not experience recurrence. According to the TNM staging of gallbladder carcinoma developed by the American Joint Committee on Cancer, 56 had stage I tumor and 25 had stage II tumor; 28 had tumor thrombus and 53 had no tumor thrombus. Immunohistochemistry was used to measure the expression of NF-κB p65, and its association with other factors was analyzed. On the basis of the localization of the protein to be measured, a comprehensive evaluation was performed based on the intensity of positive signal and the number of positive cells.The chi-square test was used for comparison of categorical data between groups, the Kaplan-Meier method was used for survival analysis, and the log-rank test was used for the comparison of survival rates. Results NF-κB p65 protein was located in the cytoplasm and some nuclei in cancer tissue and adjacent tissue, with positive rates of 90. 12% in the cancer tissue and 69. 14% in the adjacent tissue, and there was a significant difference in positive rate between the cancer tissue and the adjacent tissue ( χ2= 10. 998, P = 0. 001) . The expression of NF-κB p65 protein in cancer tissue was not associated with clinical stage, tumor thrombus, or the presence or absence of recurrence ( all P > 0. 05) . Conclusion HCC tissue has higher expression of NF-κB p65 than adjacent tissue. The key to the regulation of NF-κB function is to inhibit the promotional effect of Rel A ( p65) on liver tumor. Therefore, inhibiting the activity of NF-κB p65 may suppress the differentiation and growth of tumor cells, providing a new idea for clinical treatment of HCC.
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Key words:
- carcinoma, hepatocellular /
- NF-kappa B /
- transcription factor RelA
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