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Effect of ulinastatin combined with ischemic preconditioning on hepatic ischemia-reperfusion injury in rats
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摘要:
目的探讨乌司他丁（UTI）预处理和缺血预处理（IPC）联合应用对大鼠肝缺血再灌注损伤的影响及可能的作用机制。方法选择雄性SD大鼠50只,随机分为5组,分别为对照（sham）组、缺血再灌注（IR）组、IPC组、UTI组、UTI联合缺血预处理（UCI）组。术后采集下腔静脉血并取肝组织标本,检测血清AST、ALT、TNFɑ,肝组织髓过氧化物酶（MPO）、NF-κB、肝组织湿干比（W/D）及光镜观察肝组织病理形态学变化。计量资料组间比较采用单因素方差分析,进一步两两比较采用LSD-t检验。结果所检测的血清ALT、AST、TNFα水平和肝组织MPO、NF-κB、W/D值,IR组、IPC组、UTI组、UCI组均明显高于sham组（P值均<0.05）,而IPC组、UTI组、UCI组均明显低于IR组（P值均<0.05）,UTI组明显低于IPC组（P值均<0.05）,UCI组明显低于IPC组、UTI组（P值均<0.05）。肝脏病理学检查示IR组、IPC组、UTI组、UCI组与sham组比较,肝组织损伤明显（P值均<0.05）,而IPC组、UTI组、UCI组均比IR组肝组织损...
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关键词:
- 乌司他丁 /
- 肝疾病 /
- 缺血预处理 /
- 再灌注损伤 /
- 大鼠,Sprague-Dawley
Abstract:Objective To investigate the effect of ulinastatin (UTI) preconditioning combined with ischemic preconditioning (IPC) on hepatic ischemia-reperfusion (IR) injury in rats and possible mechanism of action.Methods A total of 50 male Sprague-Dawley rats were randomly divided into sham-operation group, IR group, IPC group, UTI group, and UTI-IPC group (UCI group) .Blood samples were collected from the inferior vena cava after surgery and liver tissue samples were also collected.The serum levels of aspartate aminotransferase (AST) , alanine aminotransferase (ALT) , and tumor necrosis factor-α (TNF-α) , the levels of myeloperoxidase (MPO) and nuclear factor-kappa B (NF-κB) in the liver tissue, and wet/dry weight ratio were determined, and pathomorphological changes of the liver tissue were observed under a light microscope.A one-way analysis of variance was used for comparison of continuous data between groups, and the LSD-t-test was used for further comparison between two groups.Results The IR, IPC, UTI, and UCI groups had significantly higher serum levels of ALT, AST, and TNF-α, levels of MPO and NF-κB in the liver tissue, and wet/dry weight ratio than the sham-operation group (all P < 0.05) ; the IPC, UTI, and UCI groups had significantly lower levels than the IR group (all P < 0.05) , the UTI group had significantly lower levels than the IPC group (all P < 0.05) , and the UCI group had significantly lower levels than the IPC and UTI groups (all P < 0.05) .Liver pathological examination showed that compared with the sham-operation group, the IR, IPC, UTI, and UCI groups had significantly greater liver injury (all P < 0.05) , while the IPC, UTI, and UCI groups had a significantly lower degree of liver injury than the IR group (all P < 0.05) , the UTI group had significantly slighter liver injury than the IPC group (P < 0.05) , and the UCI group had significantly slighter liver injury than the IPC and UTI groups (both P < 0.05) .Conclusion Both UTI and UCI have a protective effect against hepatic IR injury, and the combination of UTI and UCI significantly enhances such protective effect, possibly by inhibiting the expression of NF-κB, reducing the release of TNF-α and MPO, and alleviating liver inflammatory response.
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Key words:
- ulinastatin /
- liver diseases /
- ischemic preconditioning /
- reperfusion injury /
- rats, Sprague-Dawley
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