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Establishment of a subcutaneous xenograft tumor model of alveolar echinococcosis in nude mice
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摘要:
目的建立裸鼠皮下泡型棘球蚴病移植瘤模型,为进一步深入研究泡型棘球蚴病奠定基础。方法选取25只裸鼠进行20%泡型棘球蚴原头节混悬液0.1 ml颈背部皮下注射接种,观察记录病灶生长状况,术后6个月行台盼蓝染色观察原头节细胞活性。结果术后8 d左右可见皮下病灶结节;模型建立3个月后裸鼠存活率为92%(23/25),泡型棘球蚴感染率为100%(23/23);少量原头节死亡,台盼蓝染色蓝色,原头节细胞活力为98%。结论泡型棘球蚴能够成功移植于裸鼠皮下,可为进一步建立泡型棘球蚴病的动物模型提供参考。
Abstract:Objective To establish a subcutaneous xenograft tumor model of alveolar echinococcosis in nude mice, and to lay a foundation for further research on alveolar echinococcosis. Methods A total of 25 nude mice were given subcutaneous injection of 0. 1 ml 20% Echinococcus multilocularis protoscolex suspension at the back of the neck. The growth of lesions was observed and recorded, and trypan blue staining was performed at 6 months after injection to observe the cell viability of protoscolices. Results Subcutaneous lesions were observed at about 8 days after injection. At 3 months after the establishment of the model, the survival rate of the nude mice was 92% ( 23/25) , and the infection rate of Echinococcus multilocularis was 100% ( 23/23) . A small number of protoscolices died and were blue on trypan blue staining, and the cell viability of protoscolices was 98%. Conclusion Echinococcus multilocularis can be successfully transplanted to the subcutaneous part of nude mice, which provides a reference for further studies on the establishment of animal models of alveolar echinococcosis.
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Key words:
- echinococcosis, hepatic /
- disease models, animal
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[1]FANG D, CHEN ZY.Diagnosis and treatment of hepatic alveolar echinococcosis[J].J Clin Hepatol, 2017, 33 (5) :990-993. (in Chinese) 方丹, 陈哲宇.肝泡状棘球蚴病的诊断和治疗[J].临床肝胆病杂志, 2017, 33 (5) :990-993. [2]AHN CS, KIM JG, HAN X, et al.Biochemical characterization of echinococcus multilocularis antigen B3 reveals insight into adaptation and maintenance of parasitic homeostasis at the host-parasite interface[J].J Proteome Res, 2017, 16 (2) :806-823. [3]YE JW, LIU H, LYU GD, et al.The expression of miR-106b-25 microRNA cluster in the liver of mice with Echinococcus multilocularis infected[J].J Clin Exp Med, 2016, 15 (23) :2296-2300. (in Chinese) 叶建蔚, 刘辉, 吕国栋, 等.miR-106b-25基因簇在小鼠肝泡型包虫病中的表达[J].临床和实验医学杂志, 2016, 15 (23) :2296-2300. [4]ECKERT J, THOMPSON RC.Historical aspects of echinococcosis[J].Adv Parasitol, 2017, 95:1-64. [5]YE P, ZHANG SL, JIE SH, et al.Research progress of human hepatocellular carcinoma models in nude mice[J].World Chin J Dig, 2006, 14 (36) :3500-3503. (in Chinese) 叶翩, 张淑玲, 揭盛华, 等.人肝癌裸鼠移植模型的研究进展[J].世界华人消化杂志, 2006, 14 (36) :3500-3503. [6]WANG H, LI J, GUO BP, et al.Establishment of secondary hydatid disease infection in mice with cystic and alveolar echinococcus cysts cultured in vitro[J].Chin J Zoohoses, 2016, 32 (9) :784-788. (in Chinese) 王慧, 李军, 郭宝平, 等.微囊法棘球蚴继发感染小鼠动物模型的建立[J].中国人兽共患病学报, 2016, 32 (9) :784-788. [7]ZHANG MY, WU WP.Advances in study of the burden of echinococcosis in China and elsewhere around the world[J].J Pathog Biol, 2017, 12 (5) :473-475. (in Chinese) 张梦媛, 伍卫平.国内外包虫病疾病负担研究进展[J].中国病原生物学杂志, 2017, 12 (5) :473-475. [8]STADELMANN B, RUFENER R, AESCHBACHER D, et al.Screening of the open source malaria box reveals an early lead compound for the treatment of alveolar echinococcosis[J].PLo S Negl Trop Dis, 2016, 10 (3) :e0004535. [9]ABUDUAINI ABLZ, WEN H.Advances in multidisciplinary individualized treatment of refractory hepatic alveolar echinococcosis[J].J Clin Hepatol, 2015, 31 (4) :639-641. (in Chinese) 阿卜杜艾尼·啊卜力孜, 温浩.难治性肝泡型包虫病的多学科个体化治疗[J].临床肝胆病杂志, 2015, 31 (4) :639-641. [10]HUANG SB, MI YY, LIU AQ, et al.The status and progression in the diagnosis of hepatic echinococcosis[J].Prog Mod Biomed, 2016, 16 (4) :797-800. (in Chinese) 黄士波, 米圆圆, 刘爱琴, 等.肝包虫病的诊断现状及进展[J].现代生物医学进展, 2016, 16 (4) :797-800. [11]CHEN L, XIE ZR.Advances in radiation therapy for echinococcosis[J].J Xinjiang Med Univ, 2013, 36 (4) :557-559. (in Chinese) 陈亮, 谢增如.包虫病放射治疗的研究进展[J].新疆医科大学学报, 2013, 36 (4) :557-559. -
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