胸腺肽α1对慢性乙型肝炎CD4+CD25+CD127dim/-调节性T淋巴细胞功能的影响

李彧; 田颖; 杨晓梅; 曹宁家; 李竹; 张鸿; 张先娇

doi:10.3969/j.issn.1001-5256.2018.05.017

胸腺肽α1对慢性乙型肝炎CD4+CD25+CD127dim/-调节性T淋巴细胞功能的影响

DOI: 10.3969/j.issn.1001-5256.2018.05.017

1. 陕西省人民医院感染性疾病科
2. 陕西省中医医院妇科
3. 陕西省人民医院妇科
4. 陕西省人民医院科研处

基金项目:

陕西省自然科学基金(2013JQ4044)；

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中图分类号: R512.62
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出版历程
- 收稿日期: 2018-01-29
- 出版日期: 2018-05-20

Effect of thymosin α1 on the function of CD4+CD25+CD127dim/- regulatory T cells in chronic hepatitis B patients

Department of Infectious Diseases, Shaanxi Provincial People's Hospital

Research funding:

摘要

摘要: 目的观察胸腺肽α1对慢性乙型肝炎CD4+CD25+CD127dim/-调节性T淋巴细胞（Treg）功能的影响。方法选取2016年12月-2017年7月陕西省人民医院感染性疾病科收治的慢性乙型肝炎患者67例,其中38例接受恩替卡韦抗病毒治疗（对照组）,29例接受恩替卡韦联合注射用胸腺肽α1治疗（治疗组）,分别于治疗前后分离外周血单个核细胞,流式细胞术检测CD4+CD25+CD127dim/-Treg水平,纯化CD4+CD25+CD127dim/-Treg,与自体CD4+CD25-T淋巴细胞共培养,CCK-8法检测细胞增殖,ELISA法检测细胞因子分泌水平。计量资料2组间比较采用t检验,计数资料组间比较采用χ2检验。结果对照组、治疗组患者经治12周CD4+CD25+CD127dim/-Treg均较基线水平明显下降,差异均有统计学意...
- 肝炎,乙型,慢性 /
- T淋巴细胞,调节性 /
- 胸腺素
Abstract: Objective To investigate the effect of thymosin α1 therapy on the function of CD4+CD25+CD127dim/-regulatory T ( Treg) cells in chronic hepatitis B ( CHB) patients. Methods A total of 67 CHB patients who were admitted to Department of Infectious Diseases in Shaanxi Provincial People's Hospital from December 2016 to July 2017 were enrolled, among whom 38 patients received entecavir antiviral therapy ( control group) and 29 received entecavir combined with thymosin α1 for injection ( treatment group) . Peripheral blood mononucleated cells were isolated before and after treatment, and flow cytometry was used to measure the percentage of CD4+CD25+CD127dim/-Treg cells. CD4+CD25+CD127dim/-Treg cells were purified and co-cultured with autologous CD4+CD25-T cells. CCK-8 assay was used to measure cell proliferation, and ELISA was used to measure the levels of cytokines. The t-test was used for comparison of related indices between the two groups, the chi-square test was used for comparison of categorical data. Results In the control group, the percentage of CD4+CD25+CD127dim/-Treg cells was significantly reduced from 12. 32% ± 1. 22% at baseline to 8. 85% ± 2. 18% after 12 weeks of treatment ( t = 4. 579, P = 0. 0005) , while in the treatment group, this value was significantly reduced from 13. 71% ± 2. 32% at baseline to 9.26% ± 2. 30% after 12 weeks of treatment ( t = 4. 803, P = 0. 0003) . The measurement of cytokines was performed for 17 patients in the control group and 21 in the treatment group. After 12 weeks of treatment, the control group showed no significant changes in cell proliferation and levels of cytokines in the co-culture system of Treg and CD4+CD25-T cells; the treatment group had a significant increase in cell count in the co-culture system [ ( 3. 66 ± 0. 95) × 106 vs ( 2. 07 ± 0. 51) × 106, t = 5. 709, P < 0. 0001], as well as significant reductions in the levels of inhibitory cytokines interleukin-10 ( 41. 40 ± 11. 89 pg/ml vs 56. 53 ± 27. 85 pg/ml, t = 2. 639, P = 0. 019) and interleukin-35 ( 122. 9 ± 9. 98 pg/ml vs 130. 0 ± 15. 98 pg/ml, t = 2. 459, P = 0. 028) and significant increases in the levels of antiviral cytokines interferon-α ( 297. 5 ± 83. 56 pg/ml vs 235. 6 ± 67. 72 pg/ml, t = 2. 603, P = 0. 017) and interferon-γ ( 5. 83 ± 0. 85 pg/ml vs 4. 39 ± 0. 95 pg/ml, t = 4. 659, P = 0. 0004) . Conclusion Thymosin α1 can inhibit the level and immunosuppressive function of CD4+CD25+CD127dim/-Treg cells in CHB patients and improve their immune function.
- hepatitis B, chronic /
- T-lymphocytes, regulatory /
- thymosin

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参考文献(17)

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胸腺肽α1对慢性乙型肝炎CD4+CD25+CD127dim/-调节性T淋巴细胞功能的影响

DOI: 10.3969/j.issn.1001-5256.2018.05.017

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Effect of thymosin α1 on the function of CD4+CD25+CD127dim/- regulatory T cells in chronic hepatitis B patients

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胸腺肽α1对慢性乙型肝炎CD4+CD25+CD127dim/-调节性T淋巴细胞功能的影响

DOI: 10.3969/j.issn.1001-5256.2018.05.017

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Effect of thymosin α1 on the function of CD4+CD25+CD127dim/- regulatory T cells in chronic hepatitis B patients

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